Strain Fitness in Variovorax sp. SCN45 around GFF7048

Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7046 and GFF7047 are separated by 102 nucleotidesGFF7047 and GFF7048 are separated by 2 nucleotidesGFF7048 and GFF7049 are separated by 27 nucleotidesGFF7049 and GFF7050 are separated by 21 nucleotides GFF7046 - FIG00854755: hypothetical protein, at 7,210 to 8,406 GFF7046 GFF7047 - hypothetical protein, at 8,509 to 8,781 GFF7047 GFF7048 - hypothetical protein, at 8,784 to 9,611 GFF7048 GFF7049 - hypothetical protein, at 9,639 to 10,094 GFF7049 GFF7050 - Enoyl-CoA hydratase (EC 4.2.1.17), at 10,116 to 10,913 GFF7050 Position (kb) 8 9 10Strain fitness (log2 ratio) -1 0 1 2at 8.441 kb on + strandat 8.656 kb on + strand, within GFF7047at 8.656 kb on + strand, within GFF7047at 8.656 kb on + strand, within GFF7047at 8.657 kb on - strand, within GFF7047at 8.743 kb on + strand, within GFF7047at 8.756 kb on - strandat 8.779 kb on + strandat 8.779 kb on + strandat 8.779 kb on + strandat 8.779 kb on + strandat 8.780 kb on - strandat 9.213 kb on + strand, within GFF7048at 9.214 kb on - strand, within GFF7048at 9.404 kb on + strand, within GFF7048at 9.404 kb on + strand, within GFF7048at 9.404 kb on + strand, within GFF7048at 9.405 kb on - strand, within GFF7048at 9.405 kb on - strand, within GFF7048at 9.405 kb on - strand, within GFF7048at 9.440 kb on + strand, within GFF7048at 9.609 kb on + strandat 9.807 kb on + strand, within GFF7049at 9.949 kb on - strand, within GFF7049at 9.978 kb on + strand, within GFF7049at 9.978 kb on + strand, within GFF7049at 9.978 kb on + strand, within GFF7049at 9.978 kb on + strand, within GFF7049at 9.978 kb on + strand, within GFF7049at 9.979 kb on - strand, within GFF7049at 9.979 kb on - strand, within GFF7049at 9.979 kb on - strand, within GFF7049at 9.979 kb on - strand, within GFF7049at 9.979 kb on - strand, within GFF7049at 9.979 kb on - strand, within GFF7049at 9.979 kb on - strand, within GFF7049at 10.092 kb on + strandat 10.092 kb on - strandat 10.122 kb on + strandat 10.122 kb on + strandat 10.122 kb on + strandat 10.122 kb on + strandat 10.122 kb on + strandat 10.122 kb on + strandat 10.123 kb on - strandat 10.123 kb on - strandat 10.123 kb on - strandat 10.123 kb on - strandat 10.123 kb on - strandat 10.131 kb on + strandat 10.132 kb on - strandat 10.132 kb on - strandat 10.410 kb on + strand, within GFF7050at 10.410 kb on + strand, within GFF7050at 10.410 kb on + strand, within GFF7050at 10.411 kb on - strand, within GFF7050at 10.411 kb on - strand, within GFF7050at 10.411 kb on - strand, within GFF7050at 10.411 kb on - strand, within GFF7050at 10.500 kb on - strand, within GFF7050

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
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8,441 + -0.4
8,656 + GFF7047 0.54 +0.1
8,656 + GFF7047 0.54 -0.4
8,656 + GFF7047 0.54 -1.1
8,657 - GFF7047 0.54 -1.0
8,743 + GFF7047 0.86 -0.1
8,756 - +0.6
8,779 + +0.0
8,779 + -0.3
8,779 + -0.5
8,779 + -0.3
8,780 - +0.6
9,213 + GFF7048 0.52 -0.9
9,214 - GFF7048 0.52 +0.3
9,404 + GFF7048 0.75 -1.0
9,404 + GFF7048 0.75 -1.7
9,404 + GFF7048 0.75 -0.8
9,405 - GFF7048 0.75 -1.6
9,405 - GFF7048 0.75 -0.7
9,405 - GFF7048 0.75 -1.2
9,440 + GFF7048 0.79 -1.2
9,609 + +0.0
9,807 + GFF7049 0.37 +0.9
9,949 - GFF7049 0.68 -0.2
9,978 + GFF7049 0.74 +0.2
9,978 + GFF7049 0.74 -0.2
9,978 + GFF7049 0.74 -0.7
9,978 + GFF7049 0.74 -1.3
9,978 + GFF7049 0.74 +0.2
9,979 - GFF7049 0.75 -1.1
9,979 - GFF7049 0.75 -0.0
9,979 - GFF7049 0.75 +0.3
9,979 - GFF7049 0.75 -0.2
9,979 - GFF7049 0.75 -1.0
9,979 - GFF7049 0.75 +0.3
9,979 - GFF7049 0.75 +0.3
10,092 + +0.4
10,092 - +0.0
10,122 + +0.9
10,122 + -0.1
10,122 + +0.6
10,122 + -0.3
10,122 + -0.4
10,122 + +0.8
10,123 - -0.0
10,123 - -1.0
10,123 - -0.0
10,123 - -0.3
10,123 - -0.5
10,131 + +2.2
10,132 - -0.3
10,132 - +0.7
10,410 + GFF7050 0.37 -0.2
10,410 + GFF7050 0.37 -0.9
10,410 + GFF7050 0.37 -1.1
10,411 - GFF7050 0.37 +0.2
10,411 - GFF7050 0.37 -0.8
10,411 - GFF7050 0.37 -0.4
10,411 - GFF7050 0.37 -0.4
10,500 - GFF7050 0.48 -0.5

Or see this region's nucleotide sequence