Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7046 and GFF7047 are separated by 102 nucleotides GFF7047 and GFF7048 are separated by 2 nucleotides GFF7048 and GFF7049 are separated by 27 nucleotides GFF7049 and GFF7050 are separated by 21 nucleotides
GFF7046 - FIG00854755: hypothetical protein, at 7,210 to 8,406
GFF7046
GFF7047 - hypothetical protein, at 8,509 to 8,781
GFF7047
GFF7048 - hypothetical protein, at 8,784 to 9,611
GFF7048
GFF7049 - hypothetical protein, at 9,639 to 10,094
GFF7049
GFF7050 - Enoyl-CoA hydratase (EC 4.2.1.17), at 10,116 to 10,913
GFF7050
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-1
0
1
2 at 8.441 kb on + strand at 8.656 kb on + strand, within GFF7047 at 8.656 kb on + strand, within GFF7047 at 8.656 kb on + strand, within GFF7047 at 8.657 kb on - strand, within GFF7047 at 8.743 kb on + strand, within GFF7047 at 8.756 kb on - strand at 8.779 kb on + strand at 8.779 kb on + strand at 8.779 kb on + strand at 8.779 kb on + strand at 8.780 kb on - strand at 9.213 kb on + strand, within GFF7048 at 9.214 kb on - strand, within GFF7048 at 9.404 kb on + strand, within GFF7048 at 9.404 kb on + strand, within GFF7048 at 9.404 kb on + strand, within GFF7048 at 9.405 kb on - strand, within GFF7048 at 9.405 kb on - strand, within GFF7048 at 9.405 kb on - strand, within GFF7048 at 9.440 kb on + strand, within GFF7048 at 9.609 kb on + strand at 9.807 kb on + strand, within GFF7049 at 9.949 kb on - strand, within GFF7049 at 9.978 kb on + strand, within GFF7049 at 9.978 kb on + strand, within GFF7049 at 9.978 kb on + strand, within GFF7049 at 9.978 kb on + strand, within GFF7049 at 9.978 kb on + strand, within GFF7049 at 9.979 kb on - strand, within GFF7049 at 9.979 kb on - strand, within GFF7049 at 9.979 kb on - strand, within GFF7049 at 9.979 kb on - strand, within GFF7049 at 9.979 kb on - strand, within GFF7049 at 9.979 kb on - strand, within GFF7049 at 9.979 kb on - strand, within GFF7049 at 10.092 kb on + strand at 10.092 kb on - strand at 10.122 kb on + strand at 10.122 kb on + strand at 10.122 kb on + strand at 10.122 kb on + strand at 10.122 kb on + strand at 10.122 kb on + strand at 10.123 kb on - strand at 10.123 kb on - strand at 10.123 kb on - strand at 10.123 kb on - strand at 10.123 kb on - strand at 10.131 kb on + strand at 10.132 kb on - strand at 10.132 kb on - strand at 10.410 kb on + strand, within GFF7050 at 10.410 kb on + strand, within GFF7050 at 10.410 kb on + strand, within GFF7050 at 10.411 kb on - strand, within GFF7050 at 10.411 kb on - strand, within GFF7050 at 10.411 kb on - strand, within GFF7050 at 10.411 kb on - strand, within GFF7050 at 10.500 kb on - strand, within GFF7050
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 8,441 + -0.4 8,656 + GFF7047 0.54 +0.1 8,656 + GFF7047 0.54 -0.4 8,656 + GFF7047 0.54 -1.1 8,657 - GFF7047 0.54 -1.0 8,743 + GFF7047 0.86 -0.1 8,756 - +0.6 8,779 + +0.0 8,779 + -0.3 8,779 + -0.5 8,779 + -0.3 8,780 - +0.6 9,213 + GFF7048 0.52 -0.9 9,214 - GFF7048 0.52 +0.3 9,404 + GFF7048 0.75 -1.0 9,404 + GFF7048 0.75 -1.7 9,404 + GFF7048 0.75 -0.8 9,405 - GFF7048 0.75 -1.6 9,405 - GFF7048 0.75 -0.7 9,405 - GFF7048 0.75 -1.2 9,440 + GFF7048 0.79 -1.2 9,609 + +0.0 9,807 + GFF7049 0.37 +0.9 9,949 - GFF7049 0.68 -0.2 9,978 + GFF7049 0.74 +0.2 9,978 + GFF7049 0.74 -0.2 9,978 + GFF7049 0.74 -0.7 9,978 + GFF7049 0.74 -1.3 9,978 + GFF7049 0.74 +0.2 9,979 - GFF7049 0.75 -1.1 9,979 - GFF7049 0.75 -0.0 9,979 - GFF7049 0.75 +0.3 9,979 - GFF7049 0.75 -0.2 9,979 - GFF7049 0.75 -1.0 9,979 - GFF7049 0.75 +0.3 9,979 - GFF7049 0.75 +0.3 10,092 + +0.4 10,092 - +0.0 10,122 + +0.9 10,122 + -0.1 10,122 + +0.6 10,122 + -0.3 10,122 + -0.4 10,122 + +0.8 10,123 - -0.0 10,123 - -1.0 10,123 - -0.0 10,123 - -0.3 10,123 - -0.5 10,131 + +2.2 10,132 - -0.3 10,132 - +0.7 10,410 + GFF7050 0.37 -0.2 10,410 + GFF7050 0.37 -0.9 10,410 + GFF7050 0.37 -1.1 10,411 - GFF7050 0.37 +0.2 10,411 - GFF7050 0.37 -0.8 10,411 - GFF7050 0.37 -0.4 10,411 - GFF7050 0.37 -0.4 10,500 - GFF7050 0.48 -0.5
Or see this region's nucleotide sequence