Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF703 and GFF704 are separated by 6 nucleotides GFF704 and GFF705 are separated by 83 nucleotides
GFF703 - Efflux ABC transporter, permease/ATP-binding protein Atu2242, at 759,400 to 761,256
GFF703
GFF704 - Lactoylglutathione lyase and related lyases, at 761,263 to 761,694
GFF704
GFF705 - Transcriptional regulator, AcrR family, at 761,778 to 762,359
GFF705
Position (kb) 761
762 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 760.769 kb on - strand, within GFF703 at 761.035 kb on + strand, within GFF703 at 761.035 kb on + strand, within GFF703 at 761.035 kb on + strand, within GFF703 at 761.035 kb on + strand, within GFF703 at 761.068 kb on - strand, within GFF703 at 761.135 kb on + strand at 761.136 kb on - strand at 761.179 kb on + strand at 761.180 kb on - strand at 761.291 kb on + strand at 761.292 kb on - strand at 761.585 kb on + strand, within GFF704 at 761.585 kb on + strand, within GFF704 at 761.585 kb on + strand, within GFF704 at 761.585 kb on + strand, within GFF704 at 761.585 kb on + strand, within GFF704 at 761.585 kb on + strand, within GFF704 at 761.585 kb on + strand, within GFF704 at 761.585 kb on + strand, within GFF704 at 761.585 kb on + strand, within GFF704 at 761.585 kb on + strand, within GFF704 at 761.585 kb on + strand, within GFF704 at 761.586 kb on - strand, within GFF704 at 761.586 kb on - strand, within GFF704 at 761.586 kb on - strand, within GFF704 at 761.586 kb on - strand, within GFF704 at 761.586 kb on - strand, within GFF704 at 761.630 kb on + strand, within GFF704 at 761.630 kb on + strand, within GFF704 at 761.630 kb on + strand, within GFF704 at 761.631 kb on - strand, within GFF704 at 761.631 kb on - strand, within GFF704 at 761.631 kb on - strand, within GFF704 at 761.631 kb on - strand, within GFF704 at 761.631 kb on - strand, within GFF704 at 761.676 kb on - strand at 761.678 kb on + strand at 761.678 kb on + strand at 761.678 kb on + strand at 761.678 kb on + strand at 761.678 kb on + strand at 761.678 kb on + strand at 761.679 kb on - strand at 761.679 kb on - strand at 761.679 kb on - strand at 761.679 kb on - strand at 761.679 kb on - strand at 761.679 kb on - strand at 761.679 kb on - strand at 761.679 kb on - strand at 761.679 kb on - strand at 761.679 kb on - strand at 761.679 kb on - strand at 761.679 kb on - strand at 761.737 kb on - strand at 761.744 kb on - strand at 761.749 kb on - strand at 761.784 kb on - strand at 761.784 kb on - strand at 761.784 kb on - strand at 761.824 kb on + strand at 761.824 kb on + strand at 761.824 kb on + strand at 761.824 kb on + strand at 761.824 kb on + strand at 761.824 kb on + strand at 761.825 kb on - strand at 761.825 kb on - strand at 761.825 kb on - strand at 761.825 kb on - strand at 761.825 kb on - strand at 761.825 kb on - strand at 761.859 kb on + strand, within GFF705 at 761.919 kb on + strand, within GFF705 at 761.919 kb on + strand, within GFF705 at 761.919 kb on + strand, within GFF705 at 761.919 kb on + strand, within GFF705 at 761.920 kb on - strand, within GFF705 at 761.920 kb on - strand, within GFF705 at 761.920 kb on - strand, within GFF705 at 762.057 kb on + strand, within GFF705 at 762.058 kb on - strand, within GFF705 at 762.058 kb on - strand, within GFF705 at 762.390 kb on + strand at 762.391 kb on - strand at 762.391 kb on - strand at 762.436 kb on + strand at 762.437 kb on - strand at 762.437 kb on - strand at 762.437 kb on - strand at 762.437 kb on - strand at 762.437 kb on - strand at 762.437 kb on - strand at 762.437 kb on - strand at 762.525 kb on - strand at 762.533 kb on + strand at 762.533 kb on + strand at 762.533 kb on + strand at 762.533 kb on + strand at 762.533 kb on + strand at 762.533 kb on + strand at 762.534 kb on - strand at 762.534 kb on - strand at 762.534 kb on - strand at 762.534 kb on - strand at 762.534 kb on - strand at 762.534 kb on - strand at 762.534 kb on - strand at 762.679 kb on + strand at 762.679 kb on + strand at 762.680 kb on - strand at 762.680 kb on - strand at 762.680 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 760,769 - GFF703 0.74 -1.0 761,035 + GFF703 0.88 -0.3 761,035 + GFF703 0.88 -0.5 761,035 + GFF703 0.88 +1.5 761,035 + GFF703 0.88 -1.6 761,068 - GFF703 0.90 -0.4 761,135 + -0.6 761,136 - -0.2 761,179 + +0.8 761,180 - -0.3 761,291 + +0.1 761,292 - -1.7 761,585 + GFF704 0.75 -0.4 761,585 + GFF704 0.75 -0.2 761,585 + GFF704 0.75 +0.3 761,585 + GFF704 0.75 -1.1 761,585 + GFF704 0.75 -0.2 761,585 + GFF704 0.75 -0.9 761,585 + GFF704 0.75 +1.3 761,585 + GFF704 0.75 -1.4 761,585 + GFF704 0.75 +2.0 761,585 + GFF704 0.75 +0.3 761,585 + GFF704 0.75 +0.2 761,586 - GFF704 0.75 -0.8 761,586 - GFF704 0.75 -0.2 761,586 - GFF704 0.75 -0.3 761,586 - GFF704 0.75 -0.2 761,586 - GFF704 0.75 -1.2 761,630 + GFF704 0.85 +1.8 761,630 + GFF704 0.85 -0.3 761,630 + GFF704 0.85 -1.3 761,631 - GFF704 0.85 +3.8 761,631 - GFF704 0.85 +0.1 761,631 - GFF704 0.85 +0.7 761,631 - GFF704 0.85 +1.0 761,631 - GFF704 0.85 -0.3 761,676 - -0.4 761,678 + -0.4 761,678 + -1.0 761,678 + -0.6 761,678 + -0.8 761,678 + -0.9 761,678 + -0.5 761,679 - +2.8 761,679 - -1.9 761,679 - -0.2 761,679 - -1.2 761,679 - -0.7 761,679 - -0.4 761,679 - +0.2 761,679 - -0.8 761,679 - -0.6 761,679 - +1.1 761,679 - +0.0 761,679 - -0.7 761,737 - -1.9 761,744 - -0.6 761,749 - +0.1 761,784 - -0.5 761,784 - -0.6 761,784 - +1.0 761,824 + -0.6 761,824 + -0.1 761,824 + +1.3 761,824 + -0.1 761,824 + -0.9 761,824 + -1.2 761,825 - -0.2 761,825 - +0.2 761,825 - +0.2 761,825 - -0.2 761,825 - -0.4 761,825 - -0.2 761,859 + GFF705 0.14 +0.8 761,919 + GFF705 0.24 -0.2 761,919 + GFF705 0.24 -1.0 761,919 + GFF705 0.24 +0.3 761,919 + GFF705 0.24 +0.2 761,920 - GFF705 0.24 -0.1 761,920 - GFF705 0.24 -0.4 761,920 - GFF705 0.24 -1.2 762,057 + GFF705 0.48 -1.1 762,058 - GFF705 0.48 +0.0 762,058 - GFF705 0.48 +0.8 762,390 + +0.2 762,391 - +1.6 762,391 - +0.4 762,436 + -0.9 762,437 - -0.5 762,437 - +0.0 762,437 - -1.4 762,437 - -0.6 762,437 - -0.7 762,437 - -0.5 762,437 - -0.2 762,525 - -0.4 762,533 + -1.2 762,533 + -0.6 762,533 + -0.4 762,533 + -0.9 762,533 + +1.8 762,533 + +0.5 762,534 - +0.1 762,534 - -0.3 762,534 - +0.1 762,534 - -1.9 762,534 - -0.1 762,534 - -0.9 762,534 - +0.9 762,679 + -0.1 762,679 + -0.0 762,680 - +0.4 762,680 - +0.1 762,680 - +0.5
Or see this region's nucleotide sequence