Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6735 and GFF6736 are separated by 38 nucleotides GFF6736 and GFF6737 are separated by 212 nucleotides GFF6737 and GFF6738 overlap by 4 nucleotides
GFF6735 - hypothetical protein, at 45,879 to 47,630
GFF6735
GFF6736 - Sulfate transporter/antisigma-factor antagonist STAS:Sulphate transporter, at 47,669 to 49,354
GFF6736
GFF6737 - Uncharacterized aldehyde oxidase, 2Fe-2S subunit, at 49,567 to 50,070
GFF6737
GFF6738 - Uncharacterized aldehyde oxidase, molybdopterin-binding subunit, at 50,067 to 52,316
GFF6738
Position (kb)
47
48
49
50 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 46.701 kb on + strand, within GFF6735 at 46.701 kb on + strand, within GFF6735 at 46.701 kb on + strand, within GFF6735 at 46.702 kb on - strand, within GFF6735 at 46.702 kb on - strand, within GFF6735 at 46.959 kb on - strand, within GFF6735 at 46.981 kb on + strand, within GFF6735 at 46.981 kb on + strand, within GFF6735 at 46.981 kb on + strand, within GFF6735 at 47.004 kb on + strand, within GFF6735 at 47.005 kb on - strand, within GFF6735 at 47.019 kb on + strand, within GFF6735 at 47.020 kb on - strand, within GFF6735 at 47.060 kb on - strand, within GFF6735 at 47.060 kb on - strand, within GFF6735 at 47.060 kb on - strand, within GFF6735 at 47.060 kb on - strand, within GFF6735 at 47.071 kb on + strand, within GFF6735 at 47.071 kb on + strand, within GFF6735 at 47.071 kb on + strand, within GFF6735 at 47.071 kb on + strand, within GFF6735 at 47.071 kb on + strand, within GFF6735 at 47.071 kb on + strand, within GFF6735 at 47.071 kb on + strand, within GFF6735 at 47.072 kb on - strand, within GFF6735 at 47.072 kb on - strand, within GFF6735 at 47.072 kb on - strand, within GFF6735 at 47.072 kb on - strand, within GFF6735 at 47.073 kb on + strand, within GFF6735 at 47.074 kb on - strand, within GFF6735 at 47.074 kb on - strand, within GFF6735 at 47.074 kb on - strand, within GFF6735 at 47.171 kb on + strand, within GFF6735 at 47.171 kb on + strand, within GFF6735 at 47.172 kb on - strand, within GFF6735 at 47.237 kb on - strand, within GFF6735 at 47.349 kb on - strand, within GFF6735 at 47.434 kb on + strand, within GFF6735 at 47.455 kb on + strand at 47.456 kb on - strand at 47.456 kb on - strand at 47.456 kb on - strand at 47.456 kb on - strand at 47.456 kb on - strand at 47.515 kb on + strand at 47.554 kb on + strand at 47.555 kb on - strand at 47.555 kb on - strand at 47.628 kb on + strand at 47.628 kb on + strand at 47.629 kb on - strand at 47.629 kb on - strand at 47.629 kb on - strand at 47.670 kb on + strand at 47.718 kb on + strand at 47.718 kb on + strand at 47.718 kb on + strand at 47.718 kb on + strand at 47.719 kb on - strand at 47.719 kb on - strand at 47.719 kb on - strand at 47.719 kb on - strand at 47.757 kb on + strand at 47.757 kb on + strand at 47.757 kb on + strand at 47.757 kb on + strand at 47.757 kb on + strand at 47.758 kb on - strand at 47.758 kb on - strand at 47.758 kb on - strand at 47.758 kb on - strand at 47.758 kb on - strand at 47.859 kb on + strand, within GFF6736 at 47.927 kb on + strand, within GFF6736 at 47.928 kb on - strand, within GFF6736 at 48.078 kb on + strand, within GFF6736 at 48.252 kb on + strand, within GFF6736 at 48.442 kb on - strand, within GFF6736 at 49.179 kb on + strand, within GFF6736 at 49.179 kb on + strand, within GFF6736 at 49.179 kb on + strand, within GFF6736 at 49.245 kb on + strand at 49.246 kb on - strand at 49.246 kb on - strand at 49.246 kb on - strand at 49.246 kb on - strand at 49.246 kb on - strand at 49.520 kb on - strand at 49.575 kb on + strand at 49.576 kb on - strand at 49.576 kb on - strand at 49.576 kb on - strand at 49.582 kb on - strand at 49.902 kb on - strand, within GFF6737 at 49.902 kb on - strand, within GFF6737 at 49.935 kb on - strand, within GFF6737 at 49.971 kb on + strand, within GFF6737 at 49.971 kb on + strand, within GFF6737 at 49.972 kb on - strand, within GFF6737 at 49.975 kb on + strand, within GFF6737 at 49.975 kb on + strand, within GFF6737 at 49.975 kb on + strand, within GFF6737 at 49.976 kb on - strand, within GFF6737 at 50.310 kb on + strand, within GFF6738 at 50.310 kb on + strand, within GFF6738 at 50.310 kb on + strand, within GFF6738 at 50.311 kb on - strand, within GFF6738 at 50.311 kb on - strand, within GFF6738 at 50.311 kb on - strand, within GFF6738 at 50.311 kb on - strand, within GFF6738
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 46,701 + GFF6735 0.47 +1.3 46,701 + GFF6735 0.47 -0.6 46,701 + GFF6735 0.47 +1.5 46,702 - GFF6735 0.47 +0.1 46,702 - GFF6735 0.47 -0.2 46,959 - GFF6735 0.62 -1.3 46,981 + GFF6735 0.63 -0.4 46,981 + GFF6735 0.63 +0.2 46,981 + GFF6735 0.63 -0.5 47,004 + GFF6735 0.64 -0.0 47,005 - GFF6735 0.64 -0.2 47,019 + GFF6735 0.65 +0.8 47,020 - GFF6735 0.65 -0.6 47,060 - GFF6735 0.67 -0.5 47,060 - GFF6735 0.67 -0.4 47,060 - GFF6735 0.67 +0.6 47,060 - GFF6735 0.67 -0.0 47,071 + GFF6735 0.68 -0.4 47,071 + GFF6735 0.68 +0.1 47,071 + GFF6735 0.68 -0.6 47,071 + GFF6735 0.68 -0.5 47,071 + GFF6735 0.68 -1.6 47,071 + GFF6735 0.68 +0.4 47,071 + GFF6735 0.68 +0.1 47,072 - GFF6735 0.68 -0.6 47,072 - GFF6735 0.68 -0.2 47,072 - GFF6735 0.68 -0.1 47,072 - GFF6735 0.68 +0.2 47,073 + GFF6735 0.68 -1.0 47,074 - GFF6735 0.68 +1.2 47,074 - GFF6735 0.68 +1.1 47,074 - GFF6735 0.68 +0.5 47,171 + GFF6735 0.74 -0.4 47,171 + GFF6735 0.74 +2.1 47,172 - GFF6735 0.74 -0.2 47,237 - GFF6735 0.78 +1.1 47,349 - GFF6735 0.84 -0.6 47,434 + GFF6735 0.89 -0.3 47,455 + -0.2 47,456 - +1.1 47,456 - +1.1 47,456 - -0.2 47,456 - -0.0 47,456 - +0.5 47,515 + -0.5 47,554 + -0.3 47,555 - -0.2 47,555 - -0.1 47,628 + +0.4 47,628 + +0.5 47,629 - +0.1 47,629 - -2.3 47,629 - -0.2 47,670 + -0.2 47,718 + +0.5 47,718 + -0.4 47,718 + -0.5 47,718 + +0.6 47,719 - +0.7 47,719 - -0.1 47,719 - -0.7 47,719 - -0.6 47,757 + -0.1 47,757 + +1.1 47,757 + +0.4 47,757 + +0.8 47,757 + +0.5 47,758 - -0.7 47,758 - -0.6 47,758 - -0.3 47,758 - +1.3 47,758 - -0.7 47,859 + GFF6736 0.11 +0.1 47,927 + GFF6736 0.15 -1.3 47,928 - GFF6736 0.15 -0.4 48,078 + GFF6736 0.24 +0.3 48,252 + GFF6736 0.35 -1.0 48,442 - GFF6736 0.46 +0.5 49,179 + GFF6736 0.90 +0.2 49,179 + GFF6736 0.90 -1.0 49,179 + GFF6736 0.90 -0.7 49,245 + -1.6 49,246 - -0.0 49,246 - +0.1 49,246 - -0.5 49,246 - -0.2 49,246 - -0.8 49,520 - -1.6 49,575 + -0.3 49,576 - -1.6 49,576 - +0.8 49,576 - +0.6 49,582 - -0.2 49,902 - GFF6737 0.66 -1.7 49,902 - GFF6737 0.66 +3.1 49,935 - GFF6737 0.73 -0.6 49,971 + GFF6737 0.80 -0.6 49,971 + GFF6737 0.80 -1.1 49,972 - GFF6737 0.80 -1.2 49,975 + GFF6737 0.81 +0.7 49,975 + GFF6737 0.81 -1.1 49,975 + GFF6737 0.81 +0.0 49,976 - GFF6737 0.81 -0.5 50,310 + GFF6738 0.11 -0.6 50,310 + GFF6738 0.11 -0.7 50,310 + GFF6738 0.11 +2.7 50,311 - GFF6738 0.11 -1.4 50,311 - GFF6738 0.11 +0.5 50,311 - GFF6738 0.11 +0.1 50,311 - GFF6738 0.11 -0.5
Or see this region's nucleotide sequence