Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6634 and GFF6635 are separated by 28 nucleotides GFF6635 and GFF6636 are separated by 5 nucleotides GFF6636 and GFF6637 are separated by 15 nucleotides
GFF6634 - DNA mismatch repair protein MutS, at 5,846 to 8,443
GFF6634
GFF6635 - FIG00441227: hypothetical protein, at 8,472 to 9,311
GFF6635
GFF6636 - Hydrolase, alpha/beta fold family, at 9,317 to 10,117
GFF6636
GFF6637 - Putative amidase amiC (EC 3.5.1.4), at 10,133 to 11,605
GFF6637
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 7.584 kb on + strand, within GFF6634 at 7.584 kb on + strand, within GFF6634 at 7.585 kb on - strand, within GFF6634 at 7.585 kb on - strand, within GFF6634 at 7.585 kb on - strand, within GFF6634 at 7.585 kb on - strand, within GFF6634 at 7.585 kb on - strand, within GFF6634 at 7.585 kb on - strand, within GFF6634 at 7.720 kb on + strand, within GFF6634 at 7.720 kb on + strand, within GFF6634 at 7.720 kb on + strand, within GFF6634 at 7.720 kb on + strand, within GFF6634 at 7.720 kb on + strand, within GFF6634 at 7.721 kb on - strand, within GFF6634 at 7.730 kb on + strand, within GFF6634 at 7.730 kb on + strand, within GFF6634 at 7.730 kb on + strand, within GFF6634 at 7.730 kb on + strand, within GFF6634 at 7.730 kb on + strand, within GFF6634 at 7.730 kb on + strand, within GFF6634 at 7.730 kb on + strand, within GFF6634 at 7.730 kb on + strand, within GFF6634 at 7.730 kb on + strand, within GFF6634 at 7.730 kb on + strand, within GFF6634 at 7.731 kb on - strand, within GFF6634 at 7.731 kb on - strand, within GFF6634 at 7.731 kb on - strand, within GFF6634 at 7.731 kb on - strand, within GFF6634 at 7.731 kb on - strand, within GFF6634 at 7.731 kb on - strand, within GFF6634 at 7.731 kb on - strand, within GFF6634 at 7.731 kb on - strand, within GFF6634 at 7.731 kb on - strand, within GFF6634 at 7.731 kb on - strand, within GFF6634 at 7.731 kb on - strand, within GFF6634 at 8.048 kb on + strand, within GFF6634 at 8.048 kb on + strand, within GFF6634 at 8.048 kb on + strand, within GFF6634 at 8.048 kb on + strand, within GFF6634 at 8.048 kb on + strand, within GFF6634 at 8.048 kb on + strand, within GFF6634 at 8.048 kb on + strand, within GFF6634 at 8.049 kb on + strand, within GFF6634 at 8.049 kb on - strand, within GFF6634 at 8.049 kb on - strand, within GFF6634 at 8.049 kb on - strand, within GFF6634 at 8.174 kb on + strand, within GFF6634 at 8.174 kb on + strand, within GFF6634 at 8.174 kb on + strand, within GFF6634 at 8.174 kb on + strand, within GFF6634 at 8.174 kb on + strand, within GFF6634 at 8.174 kb on + strand, within GFF6634 at 8.174 kb on + strand, within GFF6634 at 8.175 kb on - strand, within GFF6634 at 8.175 kb on - strand, within GFF6634 at 8.175 kb on - strand, within GFF6634 at 8.175 kb on - strand, within GFF6634 at 8.175 kb on - strand, within GFF6634 at 8.334 kb on + strand at 8.334 kb on + strand at 8.334 kb on + strand at 8.334 kb on + strand at 8.334 kb on + strand at 8.335 kb on - strand at 8.335 kb on - strand at 8.335 kb on - strand at 8.399 kb on + strand at 8.399 kb on + strand at 8.399 kb on + strand at 8.399 kb on + strand at 8.399 kb on + strand at 8.399 kb on + strand at 8.399 kb on + strand at 8.399 kb on + strand at 8.400 kb on - strand at 8.400 kb on - strand at 8.400 kb on - strand at 8.400 kb on - strand at 8.400 kb on - strand at 8.400 kb on - strand at 8.400 kb on - strand at 8.400 kb on - strand at 8.400 kb on - strand at 8.400 kb on - strand at 8.400 kb on - strand at 8.478 kb on + strand at 8.478 kb on + strand at 8.478 kb on + strand at 8.478 kb on + strand at 8.478 kb on + strand at 8.478 kb on + strand at 8.479 kb on - strand at 8.479 kb on - strand at 8.479 kb on - strand at 8.479 kb on - strand at 8.479 kb on - strand at 8.479 kb on - strand at 8.485 kb on + strand at 8.485 kb on + strand at 8.485 kb on + strand at 8.485 kb on + strand at 8.485 kb on + strand at 8.486 kb on - strand at 8.486 kb on - strand at 8.486 kb on - strand at 8.486 kb on - strand at 8.486 kb on - strand at 8.486 kb on - strand at 8.486 kb on - strand at 8.486 kb on - strand at 8.486 kb on - strand at 8.580 kb on + strand, within GFF6635 at 8.580 kb on + strand, within GFF6635 at 8.581 kb on - strand, within GFF6635 at 8.781 kb on + strand, within GFF6635 at 8.781 kb on + strand, within GFF6635 at 8.781 kb on + strand, within GFF6635 at 8.782 kb on - strand, within GFF6635 at 8.782 kb on - strand, within GFF6635 at 8.782 kb on - strand, within GFF6635 at 8.782 kb on - strand, within GFF6635 at 8.782 kb on - strand, within GFF6635 at 8.886 kb on + strand, within GFF6635 at 8.886 kb on + strand, within GFF6635 at 8.886 kb on + strand, within GFF6635 at 8.886 kb on + strand, within GFF6635 at 8.887 kb on - strand, within GFF6635 at 8.887 kb on - strand, within GFF6635 at 8.887 kb on - strand, within GFF6635 at 8.887 kb on - strand, within GFF6635 at 8.887 kb on - strand, within GFF6635 at 8.928 kb on + strand, within GFF6635 at 8.928 kb on + strand, within GFF6635 at 8.928 kb on + strand, within GFF6635 at 8.928 kb on + strand, within GFF6635 at 8.929 kb on - strand, within GFF6635 at 8.929 kb on - strand, within GFF6635 at 8.929 kb on - strand, within GFF6635 at 8.929 kb on - strand, within GFF6635 at 8.929 kb on - strand, within GFF6635 at 8.929 kb on - strand, within GFF6635 at 8.929 kb on - strand, within GFF6635 at 8.952 kb on + strand, within GFF6635 at 8.953 kb on - strand, within GFF6635 at 8.953 kb on - strand, within GFF6635 at 8.953 kb on - strand, within GFF6635 at 8.953 kb on - strand, within GFF6635 at 9.087 kb on + strand, within GFF6635 at 9.087 kb on + strand, within GFF6635 at 9.087 kb on + strand, within GFF6635 at 9.087 kb on + strand, within GFF6635 at 9.087 kb on + strand, within GFF6635 at 9.087 kb on + strand, within GFF6635 at 9.087 kb on + strand, within GFF6635 at 9.087 kb on + strand, within GFF6635 at 9.087 kb on + strand, within GFF6635 at 9.088 kb on - strand, within GFF6635 at 9.088 kb on - strand, within GFF6635 at 9.088 kb on - strand, within GFF6635 at 9.088 kb on - strand, within GFF6635 at 9.088 kb on - strand, within GFF6635 at 9.088 kb on - strand, within GFF6635 at 9.088 kb on - strand, within GFF6635 at 9.088 kb on - strand, within GFF6635 at 9.088 kb on - strand, within GFF6635 at 9.144 kb on + strand, within GFF6635 at 9.144 kb on + strand, within GFF6635 at 9.144 kb on + strand, within GFF6635 at 9.145 kb on - strand, within GFF6635 at 9.145 kb on - strand, within GFF6635 at 9.147 kb on + strand, within GFF6635 at 9.148 kb on - strand, within GFF6635 at 9.148 kb on - strand, within GFF6635 at 9.148 kb on - strand, within GFF6635 at 9.148 kb on - strand, within GFF6635 at 9.230 kb on + strand at 9.230 kb on + strand at 9.231 kb on - strand at 9.365 kb on + strand at 9.365 kb on + strand at 9.365 kb on + strand at 9.443 kb on + strand, within GFF6636 at 9.443 kb on + strand, within GFF6636 at 9.444 kb on - strand, within GFF6636 at 9.444 kb on - strand, within GFF6636 at 9.809 kb on + strand, within GFF6636 at 9.809 kb on + strand, within GFF6636 at 9.810 kb on - strand, within GFF6636 at 9.810 kb on - strand, within GFF6636 at 9.810 kb on - strand, within GFF6636 at 9.810 kb on - strand, within GFF6636 at 9.810 kb on - strand, within GFF6636 at 9.810 kb on - strand, within GFF6636 at 9.810 kb on - strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.884 kb on + strand, within GFF6636 at 9.885 kb on - strand, within GFF6636 at 9.885 kb on - strand, within GFF6636 at 9.885 kb on - strand, within GFF6636 at 9.885 kb on - strand, within GFF6636 at 9.885 kb on - strand, within GFF6636 at 9.885 kb on - strand, within GFF6636 at 9.885 kb on - strand, within GFF6636 at 9.885 kb on - strand, within GFF6636 at 9.885 kb on - strand, within GFF6636 at 9.885 kb on - strand, within GFF6636 at 9.885 kb on - strand, within GFF6636
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 7,584 + GFF6634 0.67 -0.4 7,584 + GFF6634 0.67 -0.6 7,585 - GFF6634 0.67 -1.4 7,585 - GFF6634 0.67 +0.2 7,585 - GFF6634 0.67 -0.1 7,585 - GFF6634 0.67 -0.6 7,585 - GFF6634 0.67 -0.5 7,585 - GFF6634 0.67 -0.3 7,720 + GFF6634 0.72 +0.4 7,720 + GFF6634 0.72 +0.5 7,720 + GFF6634 0.72 -0.5 7,720 + GFF6634 0.72 -0.7 7,720 + GFF6634 0.72 +0.7 7,721 - GFF6634 0.72 -1.0 7,730 + GFF6634 0.73 -0.2 7,730 + GFF6634 0.73 -0.8 7,730 + GFF6634 0.73 -0.6 7,730 + GFF6634 0.73 +0.1 7,730 + GFF6634 0.73 -0.4 7,730 + GFF6634 0.73 -0.1 7,730 + GFF6634 0.73 -0.5 7,730 + GFF6634 0.73 -0.1 7,730 + GFF6634 0.73 +0.3 7,730 + GFF6634 0.73 -0.2 7,731 - GFF6634 0.73 +0.1 7,731 - GFF6634 0.73 -0.0 7,731 - GFF6634 0.73 -0.3 7,731 - GFF6634 0.73 -1.0 7,731 - GFF6634 0.73 -0.1 7,731 - GFF6634 0.73 -1.5 7,731 - GFF6634 0.73 -1.6 7,731 - GFF6634 0.73 -0.1 7,731 - GFF6634 0.73 +1.1 7,731 - GFF6634 0.73 -0.7 7,731 - GFF6634 0.73 +0.3 8,048 + GFF6634 0.85 -0.0 8,048 + GFF6634 0.85 -0.7 8,048 + GFF6634 0.85 -0.5 8,048 + GFF6634 0.85 +0.1 8,048 + GFF6634 0.85 -0.5 8,048 + GFF6634 0.85 -0.7 8,048 + GFF6634 0.85 -0.0 8,049 + GFF6634 0.85 +0.5 8,049 - GFF6634 0.85 +1.0 8,049 - GFF6634 0.85 +0.1 8,049 - GFF6634 0.85 -1.3 8,174 + GFF6634 0.90 -0.4 8,174 + GFF6634 0.90 -1.1 8,174 + GFF6634 0.90 -0.1 8,174 + GFF6634 0.90 -0.8 8,174 + GFF6634 0.90 -0.1 8,174 + GFF6634 0.90 +0.6 8,174 + GFF6634 0.90 +1.3 8,175 - GFF6634 0.90 -1.6 8,175 - GFF6634 0.90 +0.0 8,175 - GFF6634 0.90 -0.2 8,175 - GFF6634 0.90 -0.0 8,175 - GFF6634 0.90 +0.9 8,334 + -1.0 8,334 + -1.2 8,334 + -0.6 8,334 + +1.3 8,334 + -0.5 8,335 - -0.1 8,335 - -0.3 8,335 - +0.9 8,399 + -0.1 8,399 + +0.7 8,399 + -0.1 8,399 + -1.1 8,399 + +0.3 8,399 + -0.7 8,399 + -3.4 8,399 + +0.4 8,400 - +0.1 8,400 - +0.3 8,400 - -1.6 8,400 - +0.2 8,400 - -1.1 8,400 - -0.7 8,400 - +1.0 8,400 - -0.6 8,400 - -0.5 8,400 - -1.7 8,400 - +0.1 8,478 + -1.3 8,478 + +0.1 8,478 + +1.4 8,478 + -0.3 8,478 + +0.4 8,478 + -0.3 8,479 - -0.0 8,479 - -0.5 8,479 - +0.0 8,479 - +0.1 8,479 - +0.0 8,479 - -1.6 8,485 + -1.0 8,485 + +1.6 8,485 + -0.9 8,485 + -0.1 8,485 + -0.4 8,486 - -0.6 8,486 - +0.3 8,486 - -0.6 8,486 - -0.2 8,486 - +0.2 8,486 - -1.0 8,486 - +0.0 8,486 - -0.8 8,486 - +0.9 8,580 + GFF6635 0.13 -0.6 8,580 + GFF6635 0.13 +0.6 8,581 - GFF6635 0.13 +0.1 8,781 + GFF6635 0.37 -1.1 8,781 + GFF6635 0.37 -0.1 8,781 + GFF6635 0.37 -0.1 8,782 - GFF6635 0.37 +0.1 8,782 - GFF6635 0.37 -0.5 8,782 - GFF6635 0.37 +0.5 8,782 - GFF6635 0.37 -1.6 8,782 - GFF6635 0.37 -0.3 8,886 + GFF6635 0.49 -0.3 8,886 + GFF6635 0.49 -1.5 8,886 + GFF6635 0.49 +0.4 8,886 + GFF6635 0.49 -0.4 8,887 - GFF6635 0.49 -0.7 8,887 - GFF6635 0.49 +0.9 8,887 - GFF6635 0.49 +0.7 8,887 - GFF6635 0.49 -1.1 8,887 - GFF6635 0.49 +1.3 8,928 + GFF6635 0.54 -2.5 8,928 + GFF6635 0.54 -0.0 8,928 + GFF6635 0.54 -0.9 8,928 + GFF6635 0.54 -1.0 8,929 - GFF6635 0.54 -0.6 8,929 - GFF6635 0.54 +0.4 8,929 - GFF6635 0.54 +0.2 8,929 - GFF6635 0.54 +0.2 8,929 - GFF6635 0.54 +0.0 8,929 - GFF6635 0.54 -2.2 8,929 - GFF6635 0.54 -0.7 8,952 + GFF6635 0.57 +1.1 8,953 - GFF6635 0.57 +0.0 8,953 - GFF6635 0.57 +0.5 8,953 - GFF6635 0.57 -0.8 8,953 - GFF6635 0.57 +0.7 9,087 + GFF6635 0.73 -0.7 9,087 + GFF6635 0.73 -0.1 9,087 + GFF6635 0.73 +0.1 9,087 + GFF6635 0.73 -1.6 9,087 + GFF6635 0.73 -1.0 9,087 + GFF6635 0.73 +0.3 9,087 + GFF6635 0.73 +0.4 9,087 + GFF6635 0.73 -0.8 9,087 + GFF6635 0.73 +0.5 9,088 - GFF6635 0.73 +1.0 9,088 - GFF6635 0.73 -0.2 9,088 - GFF6635 0.73 -0.7 9,088 - GFF6635 0.73 +1.0 9,088 - GFF6635 0.73 -0.7 9,088 - GFF6635 0.73 -0.4 9,088 - GFF6635 0.73 +0.8 9,088 - GFF6635 0.73 -0.5 9,088 - GFF6635 0.73 -0.1 9,144 + GFF6635 0.80 +1.3 9,144 + GFF6635 0.80 -0.5 9,144 + GFF6635 0.80 -0.2 9,145 - GFF6635 0.80 -1.8 9,145 - GFF6635 0.80 -0.9 9,147 + GFF6635 0.80 +1.2 9,148 - GFF6635 0.80 +0.3 9,148 - GFF6635 0.80 +2.4 9,148 - GFF6635 0.80 +0.4 9,148 - GFF6635 0.80 +0.9 9,230 + -1.3 9,230 + +1.4 9,231 - +0.2 9,365 + +0.7 9,365 + -0.5 9,365 + -1.3 9,443 + GFF6636 0.16 -1.6 9,443 + GFF6636 0.16 -1.6 9,444 - GFF6636 0.16 -0.6 9,444 - GFF6636 0.16 -0.4 9,809 + GFF6636 0.61 -0.0 9,809 + GFF6636 0.61 -1.4 9,810 - GFF6636 0.62 +0.4 9,810 - GFF6636 0.62 -1.3 9,810 - GFF6636 0.62 -0.0 9,810 - GFF6636 0.62 -0.1 9,810 - GFF6636 0.62 -0.3 9,810 - GFF6636 0.62 +0.5 9,810 - GFF6636 0.62 -0.4 9,884 + GFF6636 0.71 -0.2 9,884 + GFF6636 0.71 +0.5 9,884 + GFF6636 0.71 -0.8 9,884 + GFF6636 0.71 -1.0 9,884 + GFF6636 0.71 -1.2 9,884 + GFF6636 0.71 -1.1 9,884 + GFF6636 0.71 -0.4 9,884 + GFF6636 0.71 +0.2 9,884 + GFF6636 0.71 -1.0 9,884 + GFF6636 0.71 -0.4 9,884 + GFF6636 0.71 -0.6 9,884 + GFF6636 0.71 -0.7 9,884 + GFF6636 0.71 +0.1 9,884 + GFF6636 0.71 -0.2 9,884 + GFF6636 0.71 -0.6 9,885 - GFF6636 0.71 -0.7 9,885 - GFF6636 0.71 -1.1 9,885 - GFF6636 0.71 -0.1 9,885 - GFF6636 0.71 -1.2 9,885 - GFF6636 0.71 -1.1 9,885 - GFF6636 0.71 -0.1 9,885 - GFF6636 0.71 +0.5 9,885 - GFF6636 0.71 +1.4 9,885 - GFF6636 0.71 +0.1 9,885 - GFF6636 0.71 +0.3 9,885 - GFF6636 0.71 -0.3
Or see this region's nucleotide sequence