Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6295 and GFF6296 are separated by 130 nucleotides GFF6296 and GFF6297 are separated by 62 nucleotides GFF6297 and GFF6298 are separated by 65 nucleotides
GFF6295 - Arginyl-tRNA synthetase (EC 6.1.1.19), at 84,380 to 86,065
GFF6295
GFF6296 - FIG00443752: hypothetical protein, at 86,196 to 86,528
GFF6296
GFF6297 - Putative transmembrane protein, at 86,591 to 87,043
GFF6297
GFF6298 - Transcriptional regulator, LysR family, at 87,109 to 88,035
GFF6298
Position (kb) 86
87
88 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 86.135 kb on + strand at 86.135 kb on + strand at 86.136 kb on - strand at 86.136 kb on - strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.189 kb on + strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.190 kb on - strand at 86.192 kb on - strand at 86.192 kb on - strand at 86.218 kb on + strand at 86.219 kb on - strand at 86.219 kb on - strand at 86.250 kb on + strand, within GFF6296 at 86.250 kb on + strand, within GFF6296 at 86.592 kb on + strand at 86.592 kb on + strand at 86.592 kb on + strand at 86.592 kb on + strand at 86.592 kb on + strand at 86.592 kb on + strand at 86.592 kb on + strand at 86.592 kb on + strand at 86.592 kb on + strand at 86.593 kb on - strand at 86.593 kb on - strand at 86.593 kb on - strand at 86.593 kb on - strand at 86.593 kb on - strand at 86.593 kb on - strand at 86.593 kb on - strand at 86.593 kb on - strand at 86.742 kb on + strand, within GFF6297 at 86.742 kb on + strand, within GFF6297 at 86.742 kb on + strand, within GFF6297 at 86.742 kb on + strand, within GFF6297 at 86.742 kb on + strand, within GFF6297 at 86.742 kb on + strand, within GFF6297 at 86.742 kb on + strand, within GFF6297 at 86.743 kb on - strand, within GFF6297 at 86.743 kb on - strand, within GFF6297 at 86.743 kb on - strand, within GFF6297 at 86.743 kb on - strand, within GFF6297 at 86.743 kb on - strand, within GFF6297 at 86.743 kb on - strand, within GFF6297 at 86.743 kb on - strand, within GFF6297 at 86.743 kb on - strand, within GFF6297 at 86.898 kb on + strand, within GFF6297 at 86.898 kb on + strand, within GFF6297 at 86.899 kb on - strand, within GFF6297 at 86.899 kb on - strand, within GFF6297 at 86.976 kb on + strand, within GFF6297 at 87.008 kb on + strand at 87.008 kb on + strand at 87.008 kb on + strand at 87.008 kb on + strand at 87.008 kb on + strand at 87.008 kb on + strand at 87.008 kb on + strand at 87.008 kb on + strand at 87.008 kb on + strand at 87.008 kb on + strand at 87.008 kb on + strand at 87.008 kb on + strand at 87.008 kb on + strand at 87.008 kb on + strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.009 kb on - strand at 87.018 kb on + strand at 87.018 kb on + strand at 87.018 kb on + strand at 87.018 kb on + strand at 87.019 kb on - strand at 87.019 kb on - strand at 87.019 kb on - strand at 87.019 kb on - strand at 87.019 kb on - strand at 87.060 kb on + strand at 87.060 kb on + strand at 87.060 kb on + strand at 87.060 kb on + strand at 87.060 kb on + strand at 87.060 kb on + strand at 87.060 kb on + strand at 87.060 kb on + strand at 87.060 kb on + strand at 87.060 kb on + strand at 87.061 kb on - strand at 87.061 kb on - strand at 87.061 kb on - strand at 87.061 kb on - strand at 87.061 kb on - strand at 87.061 kb on - strand at 87.061 kb on - strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.144 kb on - strand at 87.144 kb on - strand at 87.215 kb on + strand, within GFF6298 at 87.215 kb on + strand, within GFF6298 at 87.215 kb on + strand, within GFF6298 at 87.215 kb on + strand, within GFF6298 at 87.215 kb on + strand, within GFF6298 at 87.215 kb on + strand, within GFF6298 at 87.216 kb on - strand, within GFF6298 at 87.216 kb on - strand, within GFF6298 at 87.216 kb on - strand, within GFF6298 at 87.216 kb on - strand, within GFF6298 at 87.216 kb on - strand, within GFF6298 at 87.418 kb on - strand, within GFF6298 at 87.418 kb on - strand, within GFF6298 at 87.418 kb on - strand, within GFF6298
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 86,135 + +0.2 86,135 + +0.3 86,136 - +0.5 86,136 - +0.7 86,189 + -1.0 86,189 + -0.2 86,189 + +0.0 86,189 + -0.8 86,189 + -0.7 86,189 + +0.1 86,189 + -0.1 86,189 + -0.4 86,189 + -0.1 86,189 + -0.6 86,189 + -2.0 86,189 + -0.5 86,189 + +0.2 86,189 + -1.6 86,189 + +0.4 86,189 + +0.0 86,189 + +0.4 86,189 + +0.9 86,189 + -0.3 86,189 + -0.2 86,189 + +0.1 86,189 + -0.7 86,189 + +0.8 86,189 + +0.5 86,189 + -0.1 86,189 + +0.0 86,189 + -0.6 86,189 + -0.5 86,189 + +0.8 86,189 + -0.5 86,189 + -2.2 86,189 + -1.1 86,189 + -0.4 86,189 + +0.0 86,189 + -0.4 86,189 + -0.1 86,189 + -0.1 86,189 + +0.1 86,190 - +0.2 86,190 - -0.8 86,190 - +1.1 86,190 - -0.6 86,190 - -1.8 86,190 - -0.3 86,190 - -0.6 86,190 - +0.3 86,190 - -0.8 86,190 - +1.0 86,190 - -1.3 86,190 - +0.1 86,190 - +0.2 86,190 - +0.1 86,190 - +0.2 86,190 - +0.2 86,190 - -0.9 86,190 - -0.7 86,190 - +0.7 86,190 - -0.4 86,190 - -1.3 86,190 - +2.8 86,190 - -0.7 86,190 - -0.2 86,190 - +1.2 86,190 - +0.0 86,190 - +0.1 86,190 - -1.8 86,190 - -1.0 86,190 - -0.3 86,190 - +0.1 86,190 - -0.2 86,190 - +0.7 86,190 - +0.6 86,190 - -2.5 86,190 - +1.1 86,190 - +0.5 86,190 - -1.0 86,190 - -0.2 86,190 - +0.7 86,190 - -0.3 86,190 - -0.3 86,190 - +0.1 86,190 - -1.2 86,190 - -0.4 86,190 - -1.2 86,190 - -0.6 86,190 - -0.4 86,190 - -0.1 86,190 - -0.5 86,190 - +1.2 86,190 - +0.5 86,190 - -1.2 86,190 - -0.1 86,190 - +0.2 86,190 - +0.4 86,190 - -0.5 86,190 - +1.2 86,190 - -0.2 86,190 - -0.9 86,190 - -0.3 86,190 - -1.2 86,190 - +0.7 86,190 - -1.7 86,190 - -1.6 86,190 - -0.9 86,190 - +1.1 86,192 - +0.7 86,192 - -0.1 86,218 + -0.5 86,219 - +0.2 86,219 - -1.3 86,250 + GFF6296 0.16 -1.2 86,250 + GFF6296 0.16 -0.3 86,592 + +1.0 86,592 + -0.3 86,592 + +0.2 86,592 + -1.9 86,592 + +0.8 86,592 + -1.0 86,592 + +1.1 86,592 + -0.2 86,592 + -0.2 86,593 - -0.4 86,593 - +0.7 86,593 - -0.5 86,593 - +0.3 86,593 - -0.8 86,593 - -0.2 86,593 - +0.6 86,593 - -0.4 86,742 + GFF6297 0.33 -1.8 86,742 + GFF6297 0.33 -0.3 86,742 + GFF6297 0.33 +0.3 86,742 + GFF6297 0.33 -0.5 86,742 + GFF6297 0.33 -0.4 86,742 + GFF6297 0.33 -0.2 86,742 + GFF6297 0.33 -0.1 86,743 - GFF6297 0.34 +1.1 86,743 - GFF6297 0.34 -0.3 86,743 - GFF6297 0.34 +0.1 86,743 - GFF6297 0.34 +0.2 86,743 - GFF6297 0.34 -0.3 86,743 - GFF6297 0.34 -0.0 86,743 - GFF6297 0.34 -1.2 86,743 - GFF6297 0.34 +1.2 86,898 + GFF6297 0.68 -0.2 86,898 + GFF6297 0.68 -0.1 86,899 - GFF6297 0.68 -0.5 86,899 - GFF6297 0.68 +0.7 86,976 + GFF6297 0.85 -0.3 87,008 + +0.1 87,008 + -0.1 87,008 + +1.2 87,008 + -1.9 87,008 + -0.3 87,008 + -0.8 87,008 + -0.4 87,008 + -1.6 87,008 + +0.7 87,008 + +0.2 87,008 + -0.4 87,008 + +0.1 87,008 + +0.1 87,008 + +1.4 87,009 - -0.1 87,009 - -1.9 87,009 - -0.3 87,009 - -1.5 87,009 - +0.4 87,009 - +2.2 87,009 - -0.0 87,009 - +0.7 87,009 - +1.5 87,009 - -1.3 87,009 - -0.2 87,009 - -0.3 87,009 - -0.4 87,009 - -0.4 87,009 - -0.1 87,009 - +1.2 87,009 - -0.4 87,018 + -0.8 87,018 + +0.0 87,018 + +0.3 87,018 + -0.0 87,019 - +0.2 87,019 - +0.7 87,019 - +0.6 87,019 - -0.7 87,019 - +1.0 87,060 + -0.5 87,060 + +0.1 87,060 + -0.9 87,060 + -0.0 87,060 + -0.0 87,060 + -0.6 87,060 + +0.2 87,060 + -1.1 87,060 + -0.9 87,060 + -1.0 87,061 - -0.3 87,061 - +1.1 87,061 - +0.7 87,061 - -1.1 87,061 - -0.9 87,061 - -1.8 87,061 - -0.0 87,110 + -0.4 87,110 + -0.8 87,110 + -0.6 87,110 + -0.1 87,110 + -0.2 87,110 + -1.1 87,110 + -0.7 87,110 + -0.1 87,110 + -0.6 87,111 - +1.6 87,111 - +0.8 87,111 - -0.0 87,111 - +1.2 87,111 - -0.6 87,111 - +0.7 87,111 - -0.1 87,111 - +0.1 87,111 - -0.3 87,144 - +0.2 87,144 - +0.2 87,215 + GFF6298 0.11 -0.4 87,215 + GFF6298 0.11 -1.8 87,215 + GFF6298 0.11 -0.5 87,215 + GFF6298 0.11 -1.1 87,215 + GFF6298 0.11 +1.2 87,215 + GFF6298 0.11 -0.2 87,216 - GFF6298 0.12 -0.5 87,216 - GFF6298 0.12 +0.8 87,216 - GFF6298 0.12 -1.9 87,216 - GFF6298 0.12 -0.3 87,216 - GFF6298 0.12 +0.0 87,418 - GFF6298 0.33 -0.2 87,418 - GFF6298 0.33 -1.1 87,418 - GFF6298 0.33 -0.8
Or see this region's nucleotide sequence