Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6262 and GFF6263 overlap by 8 nucleotides GFF6263 and GFF6264 overlap by 4 nucleotides GFF6264 and GFF6265 overlap by 19 nucleotides GFF6265 and GFF6266 are separated by 21 nucleotides
GFF6262 - Transcriptional regulator PhnF, at 54,655 to 55,410
GFF6262
GFF6263 - Uncharacterized protein Atu0170, clustered with phosphonate utilization, at 55,403 to 56,137
GFF6263
GFF6264 - Ribose 1,5-bisphosphate phosphokinase PhnN (EC 2.7.4.23), at 56,134 to 56,727
GFF6264
GFF6265 - Thiamine-monophosphate kinase (EC 2.7.4.16), at 56,709 to 57,665
GFF6265
GFF6266 - putative lipoprotein, at 57,687 to 58,367
GFF6266
Position (kb) 56
57 Strain fitness (log2 ratio) -1
0
1 at 55.216 kb on + strand, within GFF6262 at 55.216 kb on + strand, within GFF6262 at 55.216 kb on + strand, within GFF6262 at 55.216 kb on + strand, within GFF6262 at 55.352 kb on - strand at 55.917 kb on - strand, within GFF6263 at 56.152 kb on + strand at 56.152 kb on + strand at 56.153 kb on - strand at 56.153 kb on - strand at 56.153 kb on - strand at 56.368 kb on + strand, within GFF6264 at 56.369 kb on - strand, within GFF6264 at 56.440 kb on + strand, within GFF6264 at 56.440 kb on + strand, within GFF6264 at 56.441 kb on - strand, within GFF6264 at 56.592 kb on - strand, within GFF6264 at 56.697 kb on + strand at 56.697 kb on + strand at 56.698 kb on - strand at 56.698 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 55,216 + GFF6262 0.74 +0.1 55,216 + GFF6262 0.74 -1.2 55,216 + GFF6262 0.74 -0.3 55,216 + GFF6262 0.74 -0.3 55,352 - -0.1 55,917 - GFF6263 0.70 +1.3 56,152 + +0.8 56,152 + -0.8 56,153 - -0.6 56,153 - -0.3 56,153 - -0.5 56,368 + GFF6264 0.39 -0.3 56,369 - GFF6264 0.40 -1.2 56,440 + GFF6264 0.52 -0.4 56,440 + GFF6264 0.52 -1.1 56,441 - GFF6264 0.52 -0.3 56,592 - GFF6264 0.77 +0.5 56,697 + +0.1 56,697 + -0.3 56,698 - +0.2 56,698 - -0.6
Or see this region's nucleotide sequence