Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6254 and GFF6255 are separated by 105 nucleotides GFF6255 and GFF6256 overlap by 4 nucleotides GFF6256 and GFF6257 overlap by 4 nucleotides
GFF6254 - Phosphonate ABC transporter ATP-binding protein PhnC (TC 3.A.1.9.1), at 47,871 to 48,728
GFF6254
GFF6255 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase (EC 3.6.1.63), at 48,834 to 49,988
GFF6255
GFF6256 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnL (EC 2.7.8.37), at 49,985 to 50,698
GFF6256
GFF6257 - Phosphonates utilization ATP-binding protein PhnK, at 50,695 to 51,483
GFF6257
Position (kb)
48
49
50 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 47.980 kb on + strand, within GFF6254 at 47.981 kb on - strand, within GFF6254 at 48.070 kb on + strand, within GFF6254 at 48.070 kb on + strand, within GFF6254 at 48.071 kb on - strand, within GFF6254 at 49.075 kb on + strand, within GFF6255 at 49.075 kb on + strand, within GFF6255 at 49.327 kb on + strand, within GFF6255 at 49.361 kb on - strand, within GFF6255 at 49.407 kb on - strand, within GFF6255 at 49.435 kb on + strand, within GFF6255 at 49.436 kb on - strand, within GFF6255 at 49.438 kb on + strand, within GFF6255 at 49.438 kb on + strand, within GFF6255 at 49.438 kb on + strand, within GFF6255 at 49.438 kb on + strand, within GFF6255 at 49.438 kb on + strand, within GFF6255 at 49.438 kb on + strand, within GFF6255 at 49.438 kb on + strand, within GFF6255 at 49.439 kb on - strand, within GFF6255 at 49.439 kb on - strand, within GFF6255 at 49.439 kb on - strand, within GFF6255 at 49.439 kb on - strand, within GFF6255 at 49.439 kb on - strand, within GFF6255 at 49.447 kb on - strand, within GFF6255 at 49.462 kb on + strand, within GFF6255 at 50.451 kb on - strand, within GFF6256 at 50.451 kb on - strand, within GFF6256 at 50.514 kb on + strand, within GFF6256 at 50.515 kb on - strand, within GFF6256 at 50.523 kb on + strand, within GFF6256 at 50.523 kb on + strand, within GFF6256 at 50.523 kb on + strand, within GFF6256 at 50.625 kb on - strand, within GFF6256 at 50.732 kb on + strand at 50.732 kb on + strand at 50.732 kb on + strand at 50.733 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 47,980 + GFF6254 0.13 -0.6 47,981 - GFF6254 0.13 -0.5 48,070 + GFF6254 0.23 -0.9 48,070 + GFF6254 0.23 +0.4 48,071 - GFF6254 0.23 -0.2 49,075 + GFF6255 0.21 -0.5 49,075 + GFF6255 0.21 +0.3 49,327 + GFF6255 0.43 -0.8 49,361 - GFF6255 0.46 +0.1 49,407 - GFF6255 0.50 -0.4 49,435 + GFF6255 0.52 -1.3 49,436 - GFF6255 0.52 -0.1 49,438 + GFF6255 0.52 -2.1 49,438 + GFF6255 0.52 -0.5 49,438 + GFF6255 0.52 -0.3 49,438 + GFF6255 0.52 -0.6 49,438 + GFF6255 0.52 -0.1 49,438 + GFF6255 0.52 +0.4 49,438 + GFF6255 0.52 +0.2 49,439 - GFF6255 0.52 -0.3 49,439 - GFF6255 0.52 +0.7 49,439 - GFF6255 0.52 -0.0 49,439 - GFF6255 0.52 -1.0 49,439 - GFF6255 0.52 -0.7 49,447 - GFF6255 0.53 -1.8 49,462 + GFF6255 0.54 -0.0 50,451 - GFF6256 0.65 -0.4 50,451 - GFF6256 0.65 -0.8 50,514 + GFF6256 0.74 -0.6 50,515 - GFF6256 0.74 +0.7 50,523 + GFF6256 0.75 +1.1 50,523 + GFF6256 0.75 -3.0 50,523 + GFF6256 0.75 -0.6 50,625 - GFF6256 0.90 -0.6 50,732 + +0.9 50,732 + +1.8 50,732 + -0.5 50,733 - -0.6
Or see this region's nucleotide sequence