Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6229 and GFF6230 are separated by 13 nucleotides GFF6230 and GFF6231 are separated by 100 nucleotides GFF6231 and GFF6232 are separated by 9 nucleotides
GFF6229 - hypothetical protein, at 12,567 to 14,549
GFF6229
GFF6230 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 14,563 to 15,540
GFF6230
GFF6231 - Amidases related to nicotinamidase, at 15,641 to 16,273
GFF6231
GFF6232 - hypothetical protein, at 16,283 to 21,721
GFF6232
Position (kb) 14
15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 13.965 kb on + strand, within GFF6229 at 13.965 kb on + strand, within GFF6229 at 13.965 kb on + strand, within GFF6229 at 13.965 kb on + strand, within GFF6229 at 13.965 kb on + strand, within GFF6229 at 13.965 kb on + strand, within GFF6229 at 13.966 kb on - strand, within GFF6229 at 14.316 kb on + strand, within GFF6229 at 14.317 kb on - strand, within GFF6229 at 14.317 kb on - strand, within GFF6229 at 14.930 kb on + strand, within GFF6230 at 14.930 kb on + strand, within GFF6230 at 14.931 kb on - strand, within GFF6230 at 14.931 kb on - strand, within GFF6230 at 14.931 kb on - strand, within GFF6230 at 14.931 kb on - strand, within GFF6230 at 15.086 kb on + strand, within GFF6230 at 15.086 kb on + strand, within GFF6230 at 15.086 kb on + strand, within GFF6230 at 15.086 kb on + strand, within GFF6230 at 15.087 kb on - strand, within GFF6230 at 15.087 kb on - strand, within GFF6230 at 15.087 kb on - strand, within GFF6230 at 15.087 kb on - strand, within GFF6230 at 15.087 kb on - strand, within GFF6230 at 15.087 kb on - strand, within GFF6230 at 15.087 kb on - strand, within GFF6230 at 15.087 kb on - strand, within GFF6230 at 15.087 kb on - strand, within GFF6230 at 15.810 kb on + strand, within GFF6231 at 15.811 kb on - strand, within GFF6231 at 15.998 kb on + strand, within GFF6231 at 15.999 kb on - strand, within GFF6231 at 15.999 kb on - strand, within GFF6231 at 16.031 kb on + strand, within GFF6231 at 16.032 kb on - strand, within GFF6231 at 16.032 kb on - strand, within GFF6231 at 16.032 kb on - strand, within GFF6231 at 16.095 kb on - strand, within GFF6231 at 16.095 kb on - strand, within GFF6231 at 16.455 kb on + strand at 16.455 kb on + strand at 16.456 kb on - strand at 16.456 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 13,965 + GFF6229 0.70 +0.2 13,965 + GFF6229 0.70 -0.5 13,965 + GFF6229 0.70 +1.9 13,965 + GFF6229 0.70 -1.0 13,965 + GFF6229 0.70 +0.5 13,965 + GFF6229 0.70 -0.8 13,966 - GFF6229 0.71 -0.1 14,316 + GFF6229 0.88 +1.2 14,317 - GFF6229 0.88 +0.6 14,317 - GFF6229 0.88 +0.1 14,930 + GFF6230 0.38 -1.5 14,930 + GFF6230 0.38 -0.1 14,931 - GFF6230 0.38 -2.5 14,931 - GFF6230 0.38 +0.5 14,931 - GFF6230 0.38 -0.9 14,931 - GFF6230 0.38 -2.5 15,086 + GFF6230 0.53 -0.8 15,086 + GFF6230 0.53 -0.0 15,086 + GFF6230 0.53 -0.2 15,086 + GFF6230 0.53 -1.1 15,087 - GFF6230 0.54 -0.8 15,087 - GFF6230 0.54 -0.2 15,087 - GFF6230 0.54 +1.0 15,087 - GFF6230 0.54 -1.5 15,087 - GFF6230 0.54 -0.6 15,087 - GFF6230 0.54 -1.2 15,087 - GFF6230 0.54 -2.9 15,087 - GFF6230 0.54 -0.2 15,087 - GFF6230 0.54 -0.9 15,810 + GFF6231 0.27 -0.7 15,811 - GFF6231 0.27 +1.0 15,998 + GFF6231 0.56 +0.3 15,999 - GFF6231 0.57 +0.2 15,999 - GFF6231 0.57 -1.5 16,031 + GFF6231 0.62 +0.1 16,032 - GFF6231 0.62 +0.3 16,032 - GFF6231 0.62 +1.0 16,032 - GFF6231 0.62 -0.3 16,095 - GFF6231 0.72 -0.1 16,095 - GFF6231 0.72 -1.9 16,455 + +0.1 16,455 + +1.4 16,456 - -1.2 16,456 - -0.6
Or see this region's nucleotide sequence