Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6141 and GFF6142 are separated by 41 nucleotides GFF6142 and GFF6143 overlap by 4 nucleotides
GFF6141 - 2-aminoethylphosphonate:pyruvate aminotransferase (EC 2.6.1.37), at 44,118 to 45,248
GFF6141
GFF6142 - 2-aminoethylphosphonate ABC transporter permease protein, at 45,290 to 47,023
GFF6142
GFF6143 - 2-aminoethylphosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 47,020 to 48,156
GFF6143
Position (kb)
45
46
47
48 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 44.299 kb on + strand, within GFF6141 at 44.300 kb on - strand, within GFF6141 at 44.317 kb on + strand, within GFF6141 at 44.317 kb on + strand, within GFF6141 at 44.437 kb on + strand, within GFF6141 at 44.476 kb on + strand, within GFF6141 at 44.476 kb on + strand, within GFF6141 at 44.477 kb on - strand, within GFF6141 at 44.477 kb on - strand, within GFF6141 at 44.554 kb on + strand, within GFF6141 at 44.554 kb on + strand, within GFF6141 at 44.554 kb on + strand, within GFF6141 at 44.554 kb on + strand, within GFF6141 at 44.555 kb on - strand, within GFF6141 at 44.555 kb on - strand, within GFF6141 at 44.555 kb on - strand, within GFF6141 at 44.555 kb on - strand, within GFF6141 at 44.570 kb on - strand, within GFF6141 at 44.915 kb on - strand, within GFF6141 at 44.915 kb on - strand, within GFF6141 at 44.975 kb on - strand, within GFF6141 at 45.477 kb on + strand, within GFF6142 at 45.525 kb on + strand, within GFF6142 at 45.525 kb on + strand, within GFF6142 at 45.678 kb on + strand, within GFF6142 at 45.679 kb on - strand, within GFF6142 at 45.679 kb on - strand, within GFF6142 at 45.679 kb on - strand, within GFF6142 at 45.681 kb on + strand, within GFF6142 at 45.681 kb on + strand, within GFF6142 at 45.682 kb on - strand, within GFF6142 at 45.682 kb on - strand, within GFF6142 at 45.871 kb on - strand, within GFF6142 at 46.006 kb on - strand, within GFF6142 at 46.032 kb on + strand, within GFF6142 at 46.129 kb on - strand, within GFF6142 at 46.179 kb on + strand, within GFF6142 at 46.180 kb on - strand, within GFF6142 at 46.341 kb on + strand, within GFF6142 at 46.341 kb on + strand, within GFF6142 at 46.342 kb on - strand, within GFF6142 at 46.377 kb on + strand, within GFF6142 at 46.450 kb on - strand, within GFF6142 at 46.515 kb on + strand, within GFF6142 at 46.548 kb on + strand, within GFF6142 at 46.548 kb on + strand, within GFF6142 at 46.549 kb on - strand, within GFF6142 at 46.711 kb on - strand, within GFF6142 at 46.809 kb on + strand, within GFF6142 at 46.810 kb on - strand, within GFF6142 at 47.282 kb on + strand, within GFF6143 at 47.441 kb on + strand, within GFF6143 at 47.441 kb on + strand, within GFF6143 at 47.442 kb on - strand, within GFF6143 at 47.717 kb on + strand, within GFF6143 at 47.718 kb on - strand, within GFF6143 at 47.718 kb on - strand, within GFF6143 at 47.855 kb on + strand, within GFF6143 at 47.855 kb on + strand, within GFF6143
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 44,299 + GFF6141 0.16 +2.4 44,300 - GFF6141 0.16 -0.3 44,317 + GFF6141 0.18 +0.5 44,317 + GFF6141 0.18 -0.6 44,437 + GFF6141 0.28 +2.4 44,476 + GFF6141 0.32 -0.3 44,476 + GFF6141 0.32 -0.2 44,477 - GFF6141 0.32 -1.0 44,477 - GFF6141 0.32 -0.2 44,554 + GFF6141 0.39 -0.2 44,554 + GFF6141 0.39 +0.1 44,554 + GFF6141 0.39 -0.2 44,554 + GFF6141 0.39 -0.6 44,555 - GFF6141 0.39 +0.5 44,555 - GFF6141 0.39 -0.2 44,555 - GFF6141 0.39 -1.4 44,555 - GFF6141 0.39 -0.2 44,570 - GFF6141 0.40 -1.2 44,915 - GFF6141 0.70 -0.5 44,915 - GFF6141 0.70 +0.5 44,975 - GFF6141 0.76 +0.3 45,477 + GFF6142 0.11 +0.6 45,525 + GFF6142 0.14 -0.8 45,525 + GFF6142 0.14 -1.0 45,678 + GFF6142 0.22 -1.7 45,679 - GFF6142 0.22 -0.2 45,679 - GFF6142 0.22 -1.4 45,679 - GFF6142 0.22 -0.3 45,681 + GFF6142 0.23 -0.3 45,681 + GFF6142 0.23 -0.3 45,682 - GFF6142 0.23 -0.6 45,682 - GFF6142 0.23 -1.2 45,871 - GFF6142 0.34 -1.2 46,006 - GFF6142 0.41 -1.8 46,032 + GFF6142 0.43 -1.1 46,129 - GFF6142 0.48 -0.3 46,179 + GFF6142 0.51 +0.4 46,180 - GFF6142 0.51 +0.7 46,341 + GFF6142 0.61 -0.8 46,341 + GFF6142 0.61 -0.4 46,342 - GFF6142 0.61 -0.6 46,377 + GFF6142 0.63 -0.7 46,450 - GFF6142 0.67 -0.7 46,515 + GFF6142 0.71 -0.9 46,548 + GFF6142 0.73 -0.1 46,548 + GFF6142 0.73 -1.0 46,549 - GFF6142 0.73 -1.5 46,711 - GFF6142 0.82 +2.6 46,809 + GFF6142 0.88 +0.2 46,810 - GFF6142 0.88 -0.4 47,282 + GFF6143 0.23 -1.4 47,441 + GFF6143 0.37 +0.6 47,441 + GFF6143 0.37 +1.4 47,442 - GFF6143 0.37 +0.7 47,717 + GFF6143 0.61 +0.5 47,718 - GFF6143 0.61 -1.5 47,718 - GFF6143 0.61 -0.0 47,855 + GFF6143 0.73 -0.6 47,855 + GFF6143 0.73 +2.2
Or see this region's nucleotide sequence