Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5966 and GFF5967 are separated by 53 nucleotides GFF5967 and GFF5968 are separated by 6 nucleotides
GFF5966 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 19,099 to 21,348
GFF5966
GFF5967 - Beta-propeller domains of methanol dehydrogenase type, at 21,402 to 22,817
GFF5967
GFF5968 - no description, at 22,824 to 23,717
GFF5968
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-2
-1
0
1 at 20.452 kb on + strand, within GFF5966 at 20.452 kb on + strand, within GFF5966 at 20.452 kb on + strand, within GFF5966 at 20.453 kb on - strand, within GFF5966 at 20.455 kb on + strand, within GFF5966 at 20.456 kb on - strand, within GFF5966 at 20.543 kb on - strand, within GFF5966 at 21.534 kb on + strand at 21.535 kb on - strand at 22.296 kb on + strand, within GFF5967 at 22.296 kb on + strand, within GFF5967 at 22.297 kb on - strand, within GFF5967 at 22.297 kb on - strand, within GFF5967 at 22.308 kb on + strand, within GFF5967 at 22.338 kb on + strand, within GFF5967 at 22.338 kb on + strand, within GFF5967 at 22.338 kb on + strand, within GFF5967 at 22.338 kb on + strand, within GFF5967 at 22.338 kb on + strand, within GFF5967 at 22.339 kb on - strand, within GFF5967 at 22.339 kb on - strand, within GFF5967 at 22.339 kb on - strand, within GFF5967 at 22.339 kb on - strand, within GFF5967 at 22.339 kb on - strand, within GFF5967 at 22.392 kb on + strand, within GFF5967 at 22.392 kb on + strand, within GFF5967 at 22.815 kb on + strand at 22.815 kb on + strand at 22.816 kb on - strand at 22.816 kb on - strand at 22.825 kb on + strand at 22.826 kb on - strand at 22.826 kb on - strand at 22.826 kb on - strand at 22.826 kb on - strand at 22.826 kb on - strand at 22.826 kb on - strand at 22.826 kb on - strand at 22.852 kb on + strand at 22.852 kb on + strand at 22.853 kb on - strand at 22.853 kb on - strand at 22.900 kb on + strand at 22.900 kb on + strand at 22.900 kb on + strand at 22.900 kb on + strand at 22.900 kb on + strand at 22.901 kb on - strand at 22.901 kb on - strand at 23.200 kb on + strand, within GFF5968 at 23.200 kb on + strand, within GFF5968 at 23.201 kb on - strand, within GFF5968 at 23.201 kb on - strand, within GFF5968 at 23.201 kb on - strand, within GFF5968 at 23.407 kb on + strand, within GFF5968 at 23.407 kb on + strand, within GFF5968 at 23.410 kb on + strand, within GFF5968 at 23.410 kb on + strand, within GFF5968 at 23.410 kb on + strand, within GFF5968 at 23.410 kb on + strand, within GFF5968 at 23.410 kb on + strand, within GFF5968 at 23.411 kb on - strand, within GFF5968 at 23.411 kb on - strand, within GFF5968 at 23.411 kb on - strand, within GFF5968 at 23.411 kb on - strand, within GFF5968 at 23.411 kb on - strand, within GFF5968 at 23.411 kb on - strand, within GFF5968 at 23.628 kb on + strand at 23.628 kb on + strand at 23.628 kb on + strand at 23.628 kb on + strand at 23.628 kb on + strand at 23.628 kb on + strand at 23.629 kb on - strand at 23.629 kb on - strand at 23.629 kb on - strand at 23.629 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 20,452 + GFF5966 0.60 +0.5 20,452 + GFF5966 0.60 +0.5 20,452 + GFF5966 0.60 -1.5 20,453 - GFF5966 0.60 -1.1 20,455 + GFF5966 0.60 +1.3 20,456 - GFF5966 0.60 +0.4 20,543 - GFF5966 0.64 +0.8 21,534 + +0.8 21,535 - -0.3 22,296 + GFF5967 0.63 -0.4 22,296 + GFF5967 0.63 -0.3 22,297 - GFF5967 0.63 -0.5 22,297 - GFF5967 0.63 +0.2 22,308 + GFF5967 0.64 +0.8 22,338 + GFF5967 0.66 -1.2 22,338 + GFF5967 0.66 +0.7 22,338 + GFF5967 0.66 -0.3 22,338 + GFF5967 0.66 -0.5 22,338 + GFF5967 0.66 -0.7 22,339 - GFF5967 0.66 -0.1 22,339 - GFF5967 0.66 -0.9 22,339 - GFF5967 0.66 -0.1 22,339 - GFF5967 0.66 -0.5 22,339 - GFF5967 0.66 -1.0 22,392 + GFF5967 0.70 +1.5 22,392 + GFF5967 0.70 -0.7 22,815 + -0.8 22,815 + -0.5 22,816 - -0.7 22,816 - -0.4 22,825 + -0.9 22,826 - -1.1 22,826 - -0.0 22,826 - -0.3 22,826 - +1.5 22,826 - -2.2 22,826 - -0.4 22,826 - -0.3 22,852 + -0.1 22,852 + -0.1 22,853 - -1.0 22,853 - +0.2 22,900 + -1.0 22,900 + +0.5 22,900 + -0.5 22,900 + -0.1 22,900 + -1.2 22,901 - +0.7 22,901 - -0.7 23,200 + GFF5968 0.42 -1.2 23,200 + GFF5968 0.42 +1.4 23,201 - GFF5968 0.42 -1.6 23,201 - GFF5968 0.42 -0.2 23,201 - GFF5968 0.42 +1.1 23,407 + GFF5968 0.65 +0.4 23,407 + GFF5968 0.65 -0.9 23,410 + GFF5968 0.66 -0.3 23,410 + GFF5968 0.66 +0.7 23,410 + GFF5968 0.66 -0.5 23,410 + GFF5968 0.66 -0.5 23,410 + GFF5968 0.66 -0.2 23,411 - GFF5968 0.66 -0.7 23,411 - GFF5968 0.66 -0.3 23,411 - GFF5968 0.66 -0.4 23,411 - GFF5968 0.66 -0.9 23,411 - GFF5968 0.66 +0.9 23,411 - GFF5968 0.66 -0.1 23,628 + -0.7 23,628 + +0.1 23,628 + -0.5 23,628 + +0.2 23,628 + -0.2 23,628 + -2.2 23,629 - -1.1 23,629 - +0.2 23,629 - -2.0 23,629 - -0.2
Or see this region's nucleotide sequence