Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF582 and GFF583 are separated by 156 nucleotides GFF583 and GFF584 are separated by 4 nucleotides GFF584 and GFF585 are separated by 73 nucleotides GFF585 and GFF586 are separated by 105 nucleotides
GFF582 - Two-component system sensor histidine kinase, at 629,207 to 630,754
GFF582
GFF583 - Tripartite tricarboxylate transporter TctB family, at 630,911 to 631,435
GFF583
GFF584 - Tripartite tricarboxylate transporter TctA family, at 631,440 to 633,014
GFF584
GFF585 - no description, at 633,088 to 633,504
GFF585
GFF586 - Pirin, at 633,610 to 634,533
GFF586
Position (kb) 631
632
633
634 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 630.449 kb on + strand, within GFF582 at 630.449 kb on + strand, within GFF582 at 630.449 kb on + strand, within GFF582 at 630.449 kb on + strand, within GFF582 at 630.450 kb on - strand, within GFF582 at 630.450 kb on - strand, within GFF582 at 630.450 kb on - strand, within GFF582 at 630.450 kb on - strand, within GFF582 at 630.780 kb on - strand at 630.794 kb on + strand at 630.795 kb on - strand at 630.802 kb on + strand at 630.802 kb on + strand at 630.802 kb on + strand at 630.802 kb on + strand at 630.810 kb on + strand at 630.810 kb on + strand at 630.810 kb on + strand at 630.810 kb on + strand at 630.811 kb on - strand at 630.811 kb on - strand at 630.811 kb on - strand at 630.811 kb on - strand at 630.811 kb on - strand at 630.811 kb on - strand at 630.811 kb on - strand at 630.811 kb on - strand at 630.811 kb on - strand at 630.811 kb on - strand at 630.811 kb on - strand at 630.811 kb on - strand at 630.816 kb on + strand at 630.816 kb on + strand at 630.878 kb on + strand at 630.910 kb on + strand at 630.910 kb on + strand at 630.910 kb on + strand at 630.910 kb on + strand at 630.910 kb on + strand at 630.910 kb on + strand at 630.910 kb on + strand at 630.910 kb on + strand at 630.910 kb on + strand at 630.910 kb on + strand at 630.910 kb on + strand at 630.910 kb on + strand at 630.910 kb on + strand at 630.910 kb on + strand at 630.910 kb on + strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.911 kb on - strand at 630.983 kb on + strand, within GFF583 at 630.983 kb on + strand, within GFF583 at 630.984 kb on - strand, within GFF583 at 631.025 kb on + strand, within GFF583 at 631.025 kb on + strand, within GFF583 at 631.025 kb on + strand, within GFF583 at 631.025 kb on + strand, within GFF583 at 631.025 kb on + strand, within GFF583 at 631.026 kb on - strand, within GFF583 at 631.026 kb on - strand, within GFF583 at 631.026 kb on - strand, within GFF583 at 631.456 kb on + strand at 631.456 kb on + strand at 631.457 kb on - strand at 631.457 kb on - strand at 631.583 kb on + strand at 631.583 kb on + strand at 631.583 kb on + strand at 631.584 kb on - strand at 631.584 kb on - strand at 631.602 kb on + strand, within GFF584 at 631.602 kb on + strand, within GFF584 at 631.602 kb on + strand, within GFF584 at 631.602 kb on + strand, within GFF584 at 631.602 kb on + strand, within GFF584 at 631.602 kb on + strand, within GFF584 at 631.603 kb on - strand, within GFF584 at 631.603 kb on - strand, within GFF584 at 631.650 kb on + strand, within GFF584 at 631.650 kb on + strand, within GFF584 at 631.651 kb on - strand, within GFF584 at 631.651 kb on - strand, within GFF584 at 631.651 kb on - strand, within GFF584 at 631.663 kb on - strand, within GFF584 at 632.283 kb on + strand, within GFF584 at 632.284 kb on - strand, within GFF584 at 632.284 kb on - strand, within GFF584 at 632.574 kb on - strand, within GFF584 at 632.610 kb on + strand, within GFF584 at 632.610 kb on + strand, within GFF584 at 632.611 kb on - strand, within GFF584 at 632.611 kb on - strand, within GFF584 at 632.611 kb on - strand, within GFF584 at 632.616 kb on + strand, within GFF584 at 632.616 kb on + strand, within GFF584 at 632.616 kb on + strand, within GFF584 at 632.616 kb on + strand, within GFF584 at 632.616 kb on + strand, within GFF584 at 632.616 kb on + strand, within GFF584 at 632.616 kb on + strand, within GFF584 at 632.616 kb on + strand, within GFF584 at 632.617 kb on - strand, within GFF584 at 632.617 kb on - strand, within GFF584 at 632.617 kb on - strand, within GFF584 at 632.617 kb on - strand, within GFF584 at 632.617 kb on - strand, within GFF584 at 632.617 kb on - strand, within GFF584 at 632.617 kb on - strand, within GFF584 at 632.617 kb on - strand, within GFF584 at 632.617 kb on - strand, within GFF584 at 632.617 kb on - strand, within GFF584 at 632.658 kb on + strand, within GFF584 at 632.658 kb on + strand, within GFF584 at 632.659 kb on - strand, within GFF584 at 632.659 kb on - strand, within GFF584 at 632.659 kb on - strand, within GFF584 at 632.905 kb on - strand at 633.012 kb on + strand at 633.012 kb on + strand at 633.012 kb on + strand at 633.012 kb on + strand at 633.012 kb on + strand at 633.012 kb on + strand at 633.013 kb on - strand at 633.013 kb on - strand at 633.013 kb on - strand at 633.013 kb on - strand at 633.013 kb on - strand at 633.013 kb on - strand at 633.090 kb on - strand at 633.090 kb on - strand at 633.233 kb on + strand, within GFF585 at 633.233 kb on + strand, within GFF585 at 633.234 kb on - strand, within GFF585 at 633.248 kb on + strand, within GFF585 at 633.248 kb on + strand, within GFF585 at 633.248 kb on + strand, within GFF585 at 633.248 kb on + strand, within GFF585 at 633.248 kb on + strand, within GFF585 at 633.248 kb on + strand, within GFF585 at 633.249 kb on - strand, within GFF585 at 633.249 kb on - strand, within GFF585 at 633.249 kb on - strand, within GFF585 at 633.249 kb on - strand, within GFF585 at 633.249 kb on - strand, within GFF585 at 633.249 kb on - strand, within GFF585 at 633.249 kb on - strand, within GFF585 at 633.249 kb on - strand, within GFF585 at 633.299 kb on + strand, within GFF585 at 633.299 kb on + strand, within GFF585 at 633.300 kb on - strand, within GFF585 at 633.365 kb on + strand, within GFF585 at 633.365 kb on + strand, within GFF585 at 633.365 kb on + strand, within GFF585 at 633.365 kb on + strand, within GFF585 at 633.365 kb on + strand, within GFF585 at 633.366 kb on - strand, within GFF585 at 633.366 kb on - strand, within GFF585 at 633.366 kb on - strand, within GFF585 at 633.366 kb on - strand, within GFF585 at 633.366 kb on - strand, within GFF585 at 633.366 kb on - strand, within GFF585 at 633.366 kb on - strand, within GFF585 at 633.465 kb on - strand at 633.489 kb on + strand at 633.490 kb on - strand at 633.490 kb on - strand at 633.490 kb on - strand at 633.490 kb on - strand at 633.504 kb on + strand at 633.504 kb on + strand at 633.504 kb on + strand at 633.504 kb on + strand at 633.504 kb on + strand at 633.504 kb on + strand at 633.504 kb on + strand at 633.504 kb on + strand at 633.504 kb on + strand at 633.504 kb on + strand at 633.505 kb on - strand at 633.505 kb on - strand at 633.505 kb on - strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.577 kb on - strand at 633.577 kb on - strand at 633.577 kb on - strand at 633.577 kb on - strand at 633.577 kb on - strand at 633.577 kb on - strand at 633.630 kb on + strand at 633.776 kb on + strand, within GFF586 at 633.776 kb on + strand, within GFF586 at 633.776 kb on + strand, within GFF586 at 633.776 kb on + strand, within GFF586 at 633.776 kb on + strand, within GFF586 at 633.777 kb on - strand, within GFF586 at 633.777 kb on - strand, within GFF586 at 633.777 kb on - strand, within GFF586 at 633.777 kb on - strand, within GFF586 at 633.777 kb on - strand, within GFF586 at 633.926 kb on + strand, within GFF586 at 633.926 kb on + strand, within GFF586 at 633.927 kb on - strand, within GFF586 at 633.927 kb on - strand, within GFF586 at 633.995 kb on + strand, within GFF586 at 633.995 kb on + strand, within GFF586 at 633.995 kb on + strand, within GFF586 at 633.996 kb on - strand, within GFF586
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 630,449 + GFF582 0.80 -0.2 630,449 + GFF582 0.80 -0.2 630,449 + GFF582 0.80 +0.5 630,449 + GFF582 0.80 -0.4 630,450 - GFF582 0.80 -0.3 630,450 - GFF582 0.80 +2.4 630,450 - GFF582 0.80 +1.8 630,450 - GFF582 0.80 +2.2 630,780 - -0.7 630,794 + +1.4 630,795 - +1.2 630,802 + -1.9 630,802 + -0.7 630,802 + -0.2 630,802 + +0.9 630,810 + -0.7 630,810 + -0.2 630,810 + -1.8 630,810 + -0.9 630,811 - -1.2 630,811 - +0.3 630,811 - -0.2 630,811 - -0.6 630,811 - -0.3 630,811 - -1.5 630,811 - -0.6 630,811 - -0.7 630,811 - -0.1 630,811 - -1.6 630,811 - +1.0 630,811 - -0.6 630,816 + -2.0 630,816 + -0.6 630,878 + -0.0 630,910 + +0.2 630,910 + +0.0 630,910 + -0.2 630,910 + +0.2 630,910 + -1.3 630,910 + -1.1 630,910 + -0.6 630,910 + -0.6 630,910 + -1.2 630,910 + -0.3 630,910 + -1.0 630,910 + -0.0 630,910 + -0.9 630,910 + +0.4 630,910 + -0.2 630,911 - +0.5 630,911 - -1.5 630,911 - -1.6 630,911 - +0.5 630,911 - +0.3 630,911 - -0.3 630,911 - -1.2 630,911 - -0.8 630,911 - -1.0 630,911 - +1.2 630,911 - +0.3 630,911 - -2.0 630,911 - -2.7 630,911 - -0.3 630,911 - -0.3 630,911 - +0.6 630,911 - -0.2 630,983 + GFF583 0.14 +1.3 630,983 + GFF583 0.14 -0.2 630,984 - GFF583 0.14 -1.2 631,025 + GFF583 0.22 +0.1 631,025 + GFF583 0.22 -0.2 631,025 + GFF583 0.22 +0.2 631,025 + GFF583 0.22 -0.4 631,025 + GFF583 0.22 +0.5 631,026 - GFF583 0.22 -0.2 631,026 - GFF583 0.22 -1.6 631,026 - GFF583 0.22 +1.5 631,456 + -0.4 631,456 + +0.4 631,457 - -0.5 631,457 - -1.0 631,583 + -0.2 631,583 + +2.0 631,583 + -0.3 631,584 - -1.3 631,584 - -0.3 631,602 + GFF584 0.10 -0.7 631,602 + GFF584 0.10 -0.2 631,602 + GFF584 0.10 +1.0 631,602 + GFF584 0.10 -0.9 631,602 + GFF584 0.10 -0.3 631,602 + GFF584 0.10 +1.4 631,603 - GFF584 0.10 +0.1 631,603 - GFF584 0.10 +0.4 631,650 + GFF584 0.13 +0.2 631,650 + GFF584 0.13 -0.4 631,651 - GFF584 0.13 +1.1 631,651 - GFF584 0.13 +0.4 631,651 - GFF584 0.13 +0.7 631,663 - GFF584 0.14 -0.6 632,283 + GFF584 0.54 +0.1 632,284 - GFF584 0.54 +0.5 632,284 - GFF584 0.54 +0.1 632,574 - GFF584 0.72 -2.1 632,610 + GFF584 0.74 +0.3 632,610 + GFF584 0.74 -0.5 632,611 - GFF584 0.74 -0.4 632,611 - GFF584 0.74 +0.3 632,611 - GFF584 0.74 -0.8 632,616 + GFF584 0.75 +1.8 632,616 + GFF584 0.75 -1.7 632,616 + GFF584 0.75 +1.0 632,616 + GFF584 0.75 -0.4 632,616 + GFF584 0.75 +0.1 632,616 + GFF584 0.75 -0.8 632,616 + GFF584 0.75 +2.2 632,616 + GFF584 0.75 -1.8 632,617 - GFF584 0.75 -2.4 632,617 - GFF584 0.75 +0.2 632,617 - GFF584 0.75 +0.1 632,617 - GFF584 0.75 -0.1 632,617 - GFF584 0.75 -0.0 632,617 - GFF584 0.75 -0.3 632,617 - GFF584 0.75 +1.1 632,617 - GFF584 0.75 +0.9 632,617 - GFF584 0.75 -0.2 632,617 - GFF584 0.75 +0.2 632,658 + GFF584 0.77 -2.1 632,658 + GFF584 0.77 +0.6 632,659 - GFF584 0.77 -0.4 632,659 - GFF584 0.77 +0.2 632,659 - GFF584 0.77 -0.1 632,905 - +1.4 633,012 + +0.0 633,012 + +0.1 633,012 + -0.7 633,012 + +0.1 633,012 + +0.3 633,012 + +1.3 633,013 - -0.4 633,013 - +1.8 633,013 - -0.1 633,013 - -0.2 633,013 - -0.0 633,013 - -0.6 633,090 - +0.8 633,090 - -0.8 633,233 + GFF585 0.35 +0.1 633,233 + GFF585 0.35 -0.7 633,234 - GFF585 0.35 +0.6 633,248 + GFF585 0.38 -1.0 633,248 + GFF585 0.38 +0.6 633,248 + GFF585 0.38 -0.0 633,248 + GFF585 0.38 +0.5 633,248 + GFF585 0.38 +1.8 633,248 + GFF585 0.38 -0.5 633,249 - GFF585 0.39 +0.2 633,249 - GFF585 0.39 -0.3 633,249 - GFF585 0.39 -0.2 633,249 - GFF585 0.39 +0.2 633,249 - GFF585 0.39 +0.6 633,249 - GFF585 0.39 +0.3 633,249 - GFF585 0.39 -0.8 633,249 - GFF585 0.39 -1.2 633,299 + GFF585 0.51 -0.8 633,299 + GFF585 0.51 -0.7 633,300 - GFF585 0.51 +0.3 633,365 + GFF585 0.66 -0.2 633,365 + GFF585 0.66 -0.2 633,365 + GFF585 0.66 -0.6 633,365 + GFF585 0.66 +1.1 633,365 + GFF585 0.66 -0.5 633,366 - GFF585 0.67 -0.8 633,366 - GFF585 0.67 -0.6 633,366 - GFF585 0.67 -0.3 633,366 - GFF585 0.67 -1.2 633,366 - GFF585 0.67 -1.0 633,366 - GFF585 0.67 +0.8 633,366 - GFF585 0.67 +0.8 633,465 - -0.6 633,489 + -0.0 633,490 - +0.6 633,490 - +0.8 633,490 - +0.4 633,490 - -0.9 633,504 + +1.8 633,504 + +2.1 633,504 + +1.8 633,504 + +0.8 633,504 + +0.3 633,504 + -1.8 633,504 + +1.0 633,504 + +0.5 633,504 + -0.5 633,504 + +0.2 633,505 - +0.9 633,505 - +0.0 633,505 - +0.3 633,576 + -0.2 633,576 + -1.2 633,576 + -0.5 633,576 + -1.0 633,576 + -0.6 633,576 + +0.1 633,576 + +2.1 633,576 + -2.0 633,576 + -0.6 633,576 + +0.8 633,576 + +0.4 633,576 + -0.4 633,576 + -1.4 633,577 - +0.8 633,577 - +0.1 633,577 - -0.9 633,577 - -0.4 633,577 - +2.8 633,577 - -1.5 633,630 + -1.0 633,776 + GFF586 0.18 -1.9 633,776 + GFF586 0.18 -0.2 633,776 + GFF586 0.18 -0.4 633,776 + GFF586 0.18 -1.7 633,776 + GFF586 0.18 +0.5 633,777 - GFF586 0.18 -0.6 633,777 - GFF586 0.18 -0.6 633,777 - GFF586 0.18 +0.2 633,777 - GFF586 0.18 +0.5 633,777 - GFF586 0.18 -0.2 633,926 + GFF586 0.34 -1.2 633,926 + GFF586 0.34 -3.0 633,927 - GFF586 0.34 -1.0 633,927 - GFF586 0.34 -0.7 633,995 + GFF586 0.42 -0.4 633,995 + GFF586 0.42 -0.3 633,995 + GFF586 0.42 -1.9 633,996 - GFF586 0.42 -0.2
Or see this region's nucleotide sequence