Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5795 and GFF5796 are separated by 70 nucleotides GFF5796 and GFF5797 are separated by 23 nucleotides GFF5797 and GFF5798 are separated by 65 nucleotides GFF5798 and GFF5799 are separated by 116 nucleotides
GFF5795 - esterase, PHB depolymerase family, at 171,054 to 172,097
GFF5795
GFF5796 - no description, at 172,168 to 172,863
GFF5796
GFF5797 - no description, at 172,887 to 173,090
GFF5797
GFF5798 - hypothetical protein, at 173,156 to 173,527
GFF5798
GFF5799 - Urea ABC transporter, substrate-binding protein UrtA, at 173,644 to 174,912
GFF5799
Position (kb) 172
173 Strain fitness (log2 ratio) -2
-1
0
1
2 at 171.253 kb on - strand, within GFF5795 at 171.453 kb on + strand, within GFF5795 at 171.454 kb on - strand, within GFF5795 at 171.528 kb on + strand, within GFF5795 at 171.528 kb on + strand, within GFF5795 at 172.159 kb on + strand at 172.169 kb on + strand at 172.169 kb on + strand at 172.169 kb on + strand at 172.169 kb on + strand at 172.169 kb on + strand at 172.169 kb on + strand at 172.170 kb on - strand at 172.170 kb on - strand at 172.170 kb on - strand at 172.173 kb on + strand at 172.173 kb on + strand at 172.174 kb on - strand at 172.174 kb on - strand at 172.174 kb on - strand at 172.179 kb on + strand at 172.180 kb on - strand at 172.275 kb on + strand, within GFF5796 at 172.345 kb on + strand, within GFF5796 at 172.345 kb on + strand, within GFF5796 at 172.367 kb on + strand, within GFF5796 at 172.368 kb on - strand, within GFF5796 at 172.368 kb on - strand, within GFF5796 at 172.368 kb on - strand, within GFF5796 at 172.368 kb on - strand, within GFF5796 at 172.393 kb on - strand, within GFF5796 at 172.393 kb on - strand, within GFF5796 at 172.419 kb on - strand, within GFF5796 at 172.419 kb on - strand, within GFF5796 at 172.510 kb on + strand, within GFF5796 at 172.511 kb on - strand, within GFF5796 at 172.556 kb on + strand, within GFF5796 at 172.556 kb on + strand, within GFF5796 at 172.556 kb on + strand, within GFF5796 at 172.557 kb on - strand, within GFF5796 at 172.557 kb on - strand, within GFF5796 at 172.557 kb on - strand, within GFF5796 at 172.557 kb on - strand, within GFF5796 at 172.557 kb on - strand, within GFF5796 at 172.560 kb on + strand, within GFF5796 at 172.560 kb on + strand, within GFF5796 at 172.560 kb on + strand, within GFF5796 at 172.561 kb on - strand, within GFF5796 at 172.561 kb on - strand, within GFF5796 at 172.562 kb on + strand, within GFF5796 at 172.588 kb on + strand, within GFF5796 at 172.590 kb on + strand, within GFF5796 at 172.590 kb on + strand, within GFF5796 at 172.590 kb on + strand, within GFF5796 at 172.590 kb on + strand, within GFF5796 at 172.590 kb on + strand, within GFF5796 at 172.617 kb on + strand, within GFF5796 at 172.617 kb on + strand, within GFF5796 at 172.618 kb on - strand, within GFF5796 at 172.618 kb on - strand, within GFF5796 at 172.618 kb on - strand, within GFF5796 at 172.618 kb on - strand, within GFF5796 at 172.618 kb on - strand, within GFF5796 at 172.618 kb on - strand, within GFF5796 at 172.634 kb on + strand, within GFF5796 at 172.635 kb on - strand, within GFF5796 at 172.635 kb on - strand, within GFF5796 at 172.635 kb on - strand, within GFF5796 at 172.635 kb on - strand, within GFF5796 at 172.686 kb on + strand, within GFF5796 at 172.687 kb on - strand, within GFF5796 at 172.775 kb on + strand, within GFF5796 at 172.775 kb on + strand, within GFF5796 at 172.775 kb on + strand, within GFF5796 at 172.776 kb on - strand, within GFF5796 at 172.776 kb on - strand, within GFF5796 at 172.916 kb on - strand, within GFF5797 at 172.922 kb on + strand, within GFF5797 at 172.923 kb on - strand, within GFF5797 at 172.923 kb on - strand, within GFF5797 at 172.997 kb on + strand, within GFF5797 at 172.998 kb on - strand, within GFF5797 at 173.031 kb on + strand, within GFF5797 at 173.032 kb on - strand, within GFF5797 at 173.061 kb on + strand, within GFF5797 at 173.128 kb on - strand at 173.128 kb on - strand at 173.148 kb on + strand at 173.168 kb on + strand at 173.168 kb on + strand at 173.168 kb on + strand at 173.168 kb on + strand at 173.168 kb on + strand at 173.168 kb on + strand at 173.169 kb on - strand at 173.169 kb on - strand at 173.169 kb on - strand at 173.426 kb on + strand, within GFF5798 at 173.426 kb on + strand, within GFF5798 at 173.427 kb on - strand, within GFF5798 at 173.507 kb on + strand at 173.508 kb on - strand at 173.508 kb on - strand at 173.530 kb on - strand at 173.539 kb on + strand at 173.540 kb on - strand at 173.540 kb on - strand at 173.553 kb on + strand at 173.554 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 171,253 - GFF5795 0.19 +0.3 171,453 + GFF5795 0.38 -0.8 171,454 - GFF5795 0.38 +0.3 171,528 + GFF5795 0.45 -0.5 171,528 + GFF5795 0.45 -0.6 172,159 + -0.9 172,169 + +1.1 172,169 + -1.3 172,169 + -0.6 172,169 + -0.6 172,169 + -0.1 172,169 + -0.3 172,170 - -0.8 172,170 - -1.7 172,170 - -0.9 172,173 + -0.5 172,173 + +0.2 172,174 - -0.7 172,174 - +0.0 172,174 - +0.1 172,179 + -0.0 172,180 - -0.5 172,275 + GFF5796 0.15 -0.0 172,345 + GFF5796 0.25 -0.5 172,345 + GFF5796 0.25 -0.2 172,367 + GFF5796 0.29 +0.5 172,368 - GFF5796 0.29 -1.8 172,368 - GFF5796 0.29 +1.7 172,368 - GFF5796 0.29 -1.1 172,368 - GFF5796 0.29 -0.3 172,393 - GFF5796 0.32 -1.4 172,393 - GFF5796 0.32 -0.6 172,419 - GFF5796 0.36 +0.3 172,419 - GFF5796 0.36 -2.2 172,510 + GFF5796 0.49 +0.0 172,511 - GFF5796 0.49 -0.8 172,556 + GFF5796 0.56 -0.6 172,556 + GFF5796 0.56 +0.2 172,556 + GFF5796 0.56 -1.1 172,557 - GFF5796 0.56 +0.3 172,557 - GFF5796 0.56 -0.1 172,557 - GFF5796 0.56 +0.1 172,557 - GFF5796 0.56 -0.4 172,557 - GFF5796 0.56 +0.1 172,560 + GFF5796 0.56 -0.9 172,560 + GFF5796 0.56 +1.1 172,560 + GFF5796 0.56 -0.0 172,561 - GFF5796 0.56 -0.1 172,561 - GFF5796 0.56 -0.8 172,562 + GFF5796 0.57 +0.1 172,588 + GFF5796 0.60 -1.0 172,590 + GFF5796 0.61 +0.1 172,590 + GFF5796 0.61 +0.3 172,590 + GFF5796 0.61 +0.3 172,590 + GFF5796 0.61 -1.1 172,590 + GFF5796 0.61 -0.8 172,617 + GFF5796 0.65 +0.0 172,617 + GFF5796 0.65 -0.4 172,618 - GFF5796 0.65 -0.2 172,618 - GFF5796 0.65 -0.8 172,618 - GFF5796 0.65 -0.2 172,618 - GFF5796 0.65 -1.3 172,618 - GFF5796 0.65 +0.5 172,618 - GFF5796 0.65 -0.4 172,634 + GFF5796 0.67 +0.9 172,635 - GFF5796 0.67 +0.2 172,635 - GFF5796 0.67 -0.3 172,635 - GFF5796 0.67 -2.1 172,635 - GFF5796 0.67 +0.2 172,686 + GFF5796 0.74 -0.0 172,687 - GFF5796 0.75 +1.0 172,775 + GFF5796 0.87 +0.5 172,775 + GFF5796 0.87 -0.4 172,775 + GFF5796 0.87 -0.4 172,776 - GFF5796 0.87 -1.6 172,776 - GFF5796 0.87 +0.8 172,916 - GFF5797 0.14 +1.1 172,922 + GFF5797 0.17 +0.3 172,923 - GFF5797 0.18 +1.3 172,923 - GFF5797 0.18 +0.0 172,997 + GFF5797 0.54 +0.4 172,998 - GFF5797 0.54 -1.7 173,031 + GFF5797 0.71 -0.4 173,032 - GFF5797 0.71 +0.1 173,061 + GFF5797 0.85 +1.8 173,128 - +0.3 173,128 - -0.2 173,148 + -1.1 173,168 + -1.3 173,168 + -0.3 173,168 + -0.4 173,168 + -0.1 173,168 + +0.9 173,168 + -0.3 173,169 - -0.3 173,169 - +0.8 173,169 - -1.0 173,426 + GFF5798 0.73 +1.1 173,426 + GFF5798 0.73 +1.6 173,427 - GFF5798 0.73 -0.5 173,507 + -0.1 173,508 - -1.1 173,508 - +0.3 173,530 - -2.0 173,539 + -0.5 173,540 - -0.2 173,540 - +2.1 173,553 + +0.9 173,554 - +0.3
Or see this region's nucleotide sequence