Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5793 and GFF5794 are separated by 145 nucleotides GFF5794 and GFF5795 are separated by 404 nucleotides
GFF5793 - Low-affinity inorganic phosphate transporter, at 167,940 to 169,577
GFF5793
GFF5794 - Polyphosphate kinase 2 (EC 2.7.4.1), at 169,723 to 170,649
GFF5794
GFF5795 - esterase, PHB depolymerase family, at 171,054 to 172,097
GFF5795
Position (kb) 169
170
171 Strain fitness (log2 ratio) -2
-1
0
1
2 at 168.853 kb on + strand, within GFF5793 at 168.853 kb on + strand, within GFF5793 at 168.854 kb on - strand, within GFF5793 at 168.854 kb on - strand, within GFF5793 at 168.943 kb on + strand, within GFF5793 at 169.283 kb on - strand, within GFF5793 at 169.283 kb on - strand, within GFF5793 at 169.650 kb on + strand at 169.650 kb on + strand at 169.650 kb on + strand at 169.651 kb on - strand at 169.651 kb on - strand at 169.673 kb on + strand at 169.673 kb on + strand at 169.673 kb on + strand at 169.673 kb on + strand at 169.673 kb on + strand at 169.673 kb on + strand at 169.673 kb on + strand at 169.673 kb on + strand at 169.674 kb on - strand at 169.674 kb on - strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.894 kb on + strand, within GFF5794 at 169.894 kb on + strand, within GFF5794 at 169.895 kb on - strand, within GFF5794 at 169.895 kb on - strand, within GFF5794 at 169.970 kb on + strand, within GFF5794 at 169.971 kb on - strand, within GFF5794 at 169.971 kb on - strand, within GFF5794 at 169.971 kb on - strand, within GFF5794 at 169.971 kb on - strand, within GFF5794 at 170.104 kb on + strand, within GFF5794 at 170.105 kb on - strand, within GFF5794 at 170.105 kb on - strand, within GFF5794 at 170.105 kb on - strand, within GFF5794 at 170.105 kb on - strand, within GFF5794 at 170.116 kb on + strand, within GFF5794 at 170.116 kb on + strand, within GFF5794 at 170.117 kb on - strand, within GFF5794 at 170.293 kb on + strand, within GFF5794 at 170.293 kb on + strand, within GFF5794 at 170.294 kb on - strand, within GFF5794 at 170.411 kb on + strand, within GFF5794 at 170.413 kb on + strand, within GFF5794 at 170.413 kb on + strand, within GFF5794 at 170.413 kb on + strand, within GFF5794 at 170.413 kb on + strand, within GFF5794 at 170.413 kb on + strand, within GFF5794 at 170.414 kb on - strand, within GFF5794 at 170.414 kb on - strand, within GFF5794 at 170.414 kb on - strand, within GFF5794 at 170.599 kb on + strand at 170.600 kb on - strand at 170.644 kb on + strand at 170.644 kb on + strand at 170.644 kb on + strand at 170.645 kb on - strand at 170.645 kb on - strand at 170.645 kb on - strand at 170.648 kb on - strand at 170.663 kb on + strand at 170.913 kb on - strand at 171.027 kb on + strand at 171.027 kb on + strand at 171.028 kb on - strand at 171.028 kb on - strand at 171.093 kb on + strand at 171.253 kb on - strand, within GFF5795 at 171.453 kb on + strand, within GFF5795 at 171.454 kb on - strand, within GFF5795 at 171.528 kb on + strand, within GFF5795 at 171.528 kb on + strand, within GFF5795
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 168,853 + GFF5793 0.56 -0.5 168,853 + GFF5793 0.56 -1.7 168,854 - GFF5793 0.56 +0.1 168,854 - GFF5793 0.56 -2.2 168,943 + GFF5793 0.61 +0.0 169,283 - GFF5793 0.82 -1.2 169,283 - GFF5793 0.82 -0.6 169,650 + +0.1 169,650 + -0.7 169,650 + -0.2 169,651 - -2.0 169,651 - -0.6 169,673 + +0.5 169,673 + +1.4 169,673 + +1.9 169,673 + +0.5 169,673 + +1.4 169,673 + +1.4 169,673 + -0.4 169,673 + -0.0 169,674 - -0.7 169,674 - +0.1 169,793 - -0.7 169,793 - -0.7 169,793 - -0.1 169,894 + GFF5794 0.18 -0.4 169,894 + GFF5794 0.18 -0.4 169,895 - GFF5794 0.19 -0.1 169,895 - GFF5794 0.19 -0.9 169,970 + GFF5794 0.27 +0.2 169,971 - GFF5794 0.27 -0.5 169,971 - GFF5794 0.27 -0.5 169,971 - GFF5794 0.27 +0.1 169,971 - GFF5794 0.27 -0.5 170,104 + GFF5794 0.41 -0.3 170,105 - GFF5794 0.41 +0.4 170,105 - GFF5794 0.41 +0.5 170,105 - GFF5794 0.41 -0.3 170,105 - GFF5794 0.41 -0.2 170,116 + GFF5794 0.42 +0.3 170,116 + GFF5794 0.42 -0.2 170,117 - GFF5794 0.43 -0.4 170,293 + GFF5794 0.61 +1.5 170,293 + GFF5794 0.61 -0.1 170,294 - GFF5794 0.62 -1.0 170,411 + GFF5794 0.74 +1.3 170,413 + GFF5794 0.74 -0.6 170,413 + GFF5794 0.74 +0.3 170,413 + GFF5794 0.74 +1.0 170,413 + GFF5794 0.74 -1.0 170,413 + GFF5794 0.74 +0.3 170,414 - GFF5794 0.75 -0.0 170,414 - GFF5794 0.75 -0.0 170,414 - GFF5794 0.75 +0.9 170,599 + -1.4 170,600 - +1.4 170,644 + +1.3 170,644 + -0.5 170,644 + -1.1 170,645 - +0.1 170,645 - +2.1 170,645 - +0.5 170,648 - -1.7 170,663 + +0.2 170,913 - +0.1 171,027 + +0.1 171,027 + -0.4 171,028 - -0.8 171,028 - -0.5 171,093 + -0.6 171,253 - GFF5795 0.19 +0.3 171,453 + GFF5795 0.38 -0.8 171,454 - GFF5795 0.38 +0.3 171,528 + GFF5795 0.45 -0.5 171,528 + GFF5795 0.45 -0.6
Or see this region's nucleotide sequence