Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5576 and GFF5577 are separated by 4 nucleotides GFF5577 and GFF5578 overlap by 4 nucleotides GFF5578 and GFF5579 are separated by 1 nucleotides
GFF5576 - Acyl-CoA dehydrogenase, at 196,430 to 197,623
GFF5576
GFF5577 - Enoyl-CoA hydratase (EC 4.2.1.17), at 197,628 to 198,482
GFF5577
GFF5578 - Transcriptional regulator, MarR family, at 198,479 to 198,958
GFF5578
GFF5579 - probable bifunctional hydroxylase/oxidoreductase, at 198,960 to 201,338
GFF5579
Position (kb) 197
198
199 Strain fitness (log2 ratio) -1
0
1 at 196.698 kb on + strand, within GFF5576 at 196.698 kb on + strand, within GFF5576 at 196.702 kb on + strand, within GFF5576 at 196.702 kb on + strand, within GFF5576 at 196.702 kb on + strand, within GFF5576 at 196.702 kb on + strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 196.703 kb on - strand, within GFF5576 at 197.094 kb on + strand, within GFF5576 at 197.095 kb on - strand, within GFF5576 at 197.095 kb on - strand, within GFF5576 at 197.095 kb on - strand, within GFF5576 at 197.095 kb on - strand, within GFF5576 at 197.263 kb on - strand, within GFF5576 at 197.263 kb on - strand, within GFF5576 at 197.299 kb on + strand, within GFF5576 at 197.355 kb on + strand, within GFF5576 at 197.356 kb on - strand, within GFF5576 at 197.437 kb on - strand, within GFF5576 at 197.674 kb on + strand at 198.046 kb on + strand, within GFF5577 at 198.289 kb on + strand, within GFF5577 at 198.289 kb on + strand, within GFF5577 at 198.289 kb on + strand, within GFF5577 at 198.290 kb on - strand, within GFF5577 at 198.394 kb on + strand, within GFF5577 at 198.395 kb on - strand, within GFF5577 at 198.421 kb on + strand at 198.422 kb on - strand at 198.422 kb on - strand at 198.422 kb on - strand at 198.472 kb on + strand at 198.472 kb on + strand at 198.473 kb on - strand at 198.473 kb on - strand at 198.473 kb on - strand at 199.027 kb on + strand at 199.028 kb on - strand at 199.028 kb on - strand at 199.028 kb on - strand at 199.051 kb on + strand at 199.051 kb on + strand at 199.051 kb on + strand at 199.052 kb on - strand at 199.052 kb on - strand at 199.052 kb on - strand at 199.264 kb on + strand, within GFF5579 at 199.264 kb on + strand, within GFF5579 at 199.264 kb on + strand, within GFF5579 at 199.265 kb on - strand, within GFF5579 at 199.265 kb on - strand, within GFF5579 at 199.265 kb on - strand, within GFF5579 at 199.276 kb on + strand, within GFF5579 at 199.276 kb on + strand, within GFF5579 at 199.276 kb on + strand, within GFF5579 at 199.277 kb on - strand, within GFF5579
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 196,698 + GFF5576 0.22 +0.1 196,698 + GFF5576 0.22 +0.0 196,702 + GFF5576 0.23 -1.0 196,702 + GFF5576 0.23 -0.7 196,702 + GFF5576 0.23 +0.8 196,702 + GFF5576 0.23 +0.6 196,703 - GFF5576 0.23 -0.6 196,703 - GFF5576 0.23 +0.5 196,703 - GFF5576 0.23 -0.6 196,703 - GFF5576 0.23 +0.9 196,703 - GFF5576 0.23 -0.5 196,703 - GFF5576 0.23 +0.6 197,094 + GFF5576 0.56 -0.4 197,095 - GFF5576 0.56 +1.1 197,095 - GFF5576 0.56 -0.4 197,095 - GFF5576 0.56 +0.1 197,095 - GFF5576 0.56 +0.2 197,263 - GFF5576 0.70 -0.6 197,263 - GFF5576 0.70 -0.7 197,299 + GFF5576 0.73 +0.8 197,355 + GFF5576 0.77 +0.9 197,356 - GFF5576 0.78 +1.2 197,437 - GFF5576 0.84 +1.4 197,674 + -0.2 198,046 + GFF5577 0.49 +0.2 198,289 + GFF5577 0.77 -1.7 198,289 + GFF5577 0.77 -0.8 198,289 + GFF5577 0.77 -0.9 198,290 - GFF5577 0.77 -0.5 198,394 + GFF5577 0.90 +0.4 198,395 - GFF5577 0.90 -0.3 198,421 + -0.8 198,422 - -0.8 198,422 - +0.1 198,422 - +1.3 198,472 + -1.3 198,472 + +0.2 198,473 - -0.4 198,473 - -0.0 198,473 - -0.8 199,027 + -0.4 199,028 - -1.1 199,028 - +0.1 199,028 - -0.2 199,051 + -0.4 199,051 + -0.8 199,051 + -0.6 199,052 - -0.7 199,052 - +0.2 199,052 - -0.8 199,264 + GFF5579 0.13 -0.9 199,264 + GFF5579 0.13 -0.3 199,264 + GFF5579 0.13 -0.7 199,265 - GFF5579 0.13 -0.5 199,265 - GFF5579 0.13 -0.5 199,265 - GFF5579 0.13 -0.8 199,276 + GFF5579 0.13 -0.6 199,276 + GFF5579 0.13 +1.4 199,276 + GFF5579 0.13 -0.0 199,277 - GFF5579 0.13 +0.4
Or see this region's nucleotide sequence