Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5295 and GFF5296 are separated by 33 nucleotides GFF5296 and GFF5297 overlap by 4 nucleotides GFF5297 and GFF5298 are separated by 171 nucleotides
GFF5295 - Ferrichrome-iron receptor, at 167,828 to 169,948
GFF5295
GFF5296 - putative iron-regulated membrane protein, at 169,982 to 171,154
GFF5296
GFF5297 - hypothetical protein, at 171,151 to 171,345
GFF5297
GFF5298 - FIG01204242: hypothetical protein, at 171,517 to 173,733
GFF5298
Position (kb)
169
170
171
172 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 169.055 kb on + strand, within GFF5295 at 169.055 kb on + strand, within GFF5295 at 169.056 kb on - strand, within GFF5295 at 169.056 kb on - strand, within GFF5295 at 169.073 kb on + strand, within GFF5295 at 169.098 kb on - strand, within GFF5295 at 169.157 kb on + strand, within GFF5295 at 169.158 kb on - strand, within GFF5295 at 169.158 kb on - strand, within GFF5295 at 169.289 kb on + strand, within GFF5295 at 169.289 kb on + strand, within GFF5295 at 169.289 kb on + strand, within GFF5295 at 169.289 kb on + strand, within GFF5295 at 169.289 kb on + strand, within GFF5295 at 169.289 kb on + strand, within GFF5295 at 169.290 kb on - strand, within GFF5295 at 169.290 kb on - strand, within GFF5295 at 169.290 kb on - strand, within GFF5295 at 169.290 kb on - strand, within GFF5295 at 169.290 kb on - strand, within GFF5295 at 169.316 kb on + strand, within GFF5295 at 169.317 kb on - strand, within GFF5295 at 169.325 kb on + strand, within GFF5295 at 169.325 kb on + strand, within GFF5295 at 169.325 kb on + strand, within GFF5295 at 169.326 kb on - strand, within GFF5295 at 169.326 kb on - strand, within GFF5295 at 169.418 kb on + strand, within GFF5295 at 169.418 kb on + strand, within GFF5295 at 169.419 kb on - strand, within GFF5295 at 169.511 kb on + strand, within GFF5295 at 169.512 kb on - strand, within GFF5295 at 169.601 kb on + strand, within GFF5295 at 169.601 kb on + strand, within GFF5295 at 169.601 kb on + strand, within GFF5295 at 169.601 kb on + strand, within GFF5295 at 169.602 kb on - strand, within GFF5295 at 169.670 kb on + strand, within GFF5295 at 169.671 kb on - strand, within GFF5295 at 169.671 kb on - strand, within GFF5295 at 169.724 kb on + strand, within GFF5295 at 169.724 kb on + strand, within GFF5295 at 169.829 kb on + strand at 169.865 kb on + strand at 169.866 kb on - strand at 169.866 kb on - strand at 169.937 kb on + strand at 169.937 kb on + strand at 169.938 kb on - strand at 170.366 kb on + strand, within GFF5296 at 170.366 kb on + strand, within GFF5296 at 170.366 kb on + strand, within GFF5296 at 170.366 kb on + strand, within GFF5296 at 170.366 kb on + strand, within GFF5296 at 170.366 kb on + strand, within GFF5296 at 170.366 kb on + strand, within GFF5296 at 170.366 kb on + strand, within GFF5296 at 170.366 kb on + strand, within GFF5296 at 170.366 kb on + strand, within GFF5296 at 170.366 kb on + strand, within GFF5296 at 170.366 kb on + strand, within GFF5296 at 170.366 kb on + strand, within GFF5296 at 170.367 kb on - strand, within GFF5296 at 170.367 kb on - strand, within GFF5296 at 170.367 kb on - strand, within GFF5296 at 170.367 kb on - strand, within GFF5296 at 170.367 kb on - strand, within GFF5296 at 170.367 kb on - strand, within GFF5296 at 170.477 kb on + strand, within GFF5296 at 170.646 kb on - strand, within GFF5296 at 170.646 kb on - strand, within GFF5296 at 170.646 kb on - strand, within GFF5296 at 170.646 kb on - strand, within GFF5296 at 170.654 kb on + strand, within GFF5296 at 170.655 kb on - strand, within GFF5296 at 170.655 kb on - strand, within GFF5296 at 170.684 kb on + strand, within GFF5296 at 170.685 kb on - strand, within GFF5296 at 170.930 kb on + strand, within GFF5296 at 170.931 kb on - strand, within GFF5296 at 170.931 kb on - strand, within GFF5296 at 170.976 kb on - strand, within GFF5296 at 171.050 kb on + strand at 171.051 kb on - strand at 171.051 kb on - strand at 171.343 kb on + strand at 171.413 kb on + strand at 171.414 kb on - strand at 171.516 kb on + strand at 171.516 kb on + strand at 171.516 kb on + strand at 171.516 kb on + strand at 171.516 kb on + strand at 171.516 kb on + strand at 171.517 kb on - strand at 171.517 kb on - strand at 171.517 kb on - strand at 171.517 kb on - strand at 171.517 kb on - strand at 171.517 kb on - strand at 171.576 kb on - strand at 171.576 kb on - strand at 171.736 kb on + strand at 171.983 kb on - strand, within GFF5298 at 171.994 kb on + strand, within GFF5298 at 171.994 kb on + strand, within GFF5298 at 171.994 kb on + strand, within GFF5298 at 171.994 kb on + strand, within GFF5298 at 171.995 kb on - strand, within GFF5298 at 171.995 kb on - strand, within GFF5298 at 172.072 kb on + strand, within GFF5298 at 172.072 kb on + strand, within GFF5298 at 172.073 kb on - strand, within GFF5298 at 172.073 kb on - strand, within GFF5298 at 172.120 kb on + strand, within GFF5298 at 172.120 kb on + strand, within GFF5298 at 172.121 kb on - strand, within GFF5298 at 172.121 kb on - strand, within GFF5298 at 172.121 kb on - strand, within GFF5298 at 172.121 kb on - strand, within GFF5298 at 172.121 kb on - strand, within GFF5298
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 169,055 + GFF5295 0.58 +1.5 169,055 + GFF5295 0.58 +0.7 169,056 - GFF5295 0.58 -0.9 169,056 - GFF5295 0.58 +0.3 169,073 + GFF5295 0.59 -0.3 169,098 - GFF5295 0.60 -0.5 169,157 + GFF5295 0.63 +0.9 169,158 - GFF5295 0.63 +0.1 169,158 - GFF5295 0.63 -1.4 169,289 + GFF5295 0.69 -1.0 169,289 + GFF5295 0.69 +0.3 169,289 + GFF5295 0.69 +0.6 169,289 + GFF5295 0.69 -1.8 169,289 + GFF5295 0.69 +0.8 169,289 + GFF5295 0.69 -0.4 169,290 - GFF5295 0.69 +0.7 169,290 - GFF5295 0.69 -1.4 169,290 - GFF5295 0.69 -1.0 169,290 - GFF5295 0.69 +0.8 169,290 - GFF5295 0.69 -0.9 169,316 + GFF5295 0.70 -0.6 169,317 - GFF5295 0.70 +0.9 169,325 + GFF5295 0.71 -1.0 169,325 + GFF5295 0.71 -0.2 169,325 + GFF5295 0.71 +1.6 169,326 - GFF5295 0.71 +0.1 169,326 - GFF5295 0.71 -2.2 169,418 + GFF5295 0.75 -0.9 169,418 + GFF5295 0.75 -0.4 169,419 - GFF5295 0.75 +1.1 169,511 + GFF5295 0.79 +0.7 169,512 - GFF5295 0.79 -1.6 169,601 + GFF5295 0.84 -0.5 169,601 + GFF5295 0.84 +1.2 169,601 + GFF5295 0.84 -0.0 169,601 + GFF5295 0.84 -1.1 169,602 - GFF5295 0.84 +0.2 169,670 + GFF5295 0.87 -0.6 169,671 - GFF5295 0.87 +1.3 169,671 - GFF5295 0.87 -1.1 169,724 + GFF5295 0.89 +0.3 169,724 + GFF5295 0.89 -0.3 169,829 + -1.3 169,865 + -0.4 169,866 - -0.7 169,866 - -0.7 169,937 + +1.7 169,937 + +0.9 169,938 - +0.3 170,366 + GFF5296 0.33 -0.8 170,366 + GFF5296 0.33 -0.0 170,366 + GFF5296 0.33 +1.8 170,366 + GFF5296 0.33 -0.7 170,366 + GFF5296 0.33 -0.6 170,366 + GFF5296 0.33 +3.1 170,366 + GFF5296 0.33 -1.6 170,366 + GFF5296 0.33 +1.6 170,366 + GFF5296 0.33 +1.4 170,366 + GFF5296 0.33 +0.3 170,366 + GFF5296 0.33 -0.4 170,366 + GFF5296 0.33 -0.2 170,366 + GFF5296 0.33 -0.4 170,367 - GFF5296 0.33 +2.5 170,367 - GFF5296 0.33 +0.3 170,367 - GFF5296 0.33 +0.0 170,367 - GFF5296 0.33 -1.5 170,367 - GFF5296 0.33 -1.4 170,367 - GFF5296 0.33 +1.6 170,477 + GFF5296 0.42 +1.3 170,646 - GFF5296 0.57 +0.6 170,646 - GFF5296 0.57 +1.9 170,646 - GFF5296 0.57 -0.7 170,646 - GFF5296 0.57 -0.7 170,654 + GFF5296 0.57 +1.4 170,655 - GFF5296 0.57 +0.9 170,655 - GFF5296 0.57 +0.3 170,684 + GFF5296 0.60 -0.4 170,685 - GFF5296 0.60 -0.1 170,930 + GFF5296 0.81 -0.2 170,931 - GFF5296 0.81 -0.7 170,931 - GFF5296 0.81 -0.3 170,976 - GFF5296 0.85 -0.4 171,050 + -0.3 171,051 - -0.2 171,051 - +2.1 171,343 + -0.3 171,413 + -0.1 171,414 - -0.7 171,516 + +0.1 171,516 + +0.8 171,516 + -0.4 171,516 + -0.2 171,516 + -0.2 171,516 + -0.9 171,517 - -0.8 171,517 - +0.8 171,517 - +0.1 171,517 - +0.6 171,517 - -2.2 171,517 - +0.7 171,576 - +0.7 171,576 - -2.4 171,736 + +2.7 171,983 - GFF5298 0.21 +0.6 171,994 + GFF5298 0.22 -1.2 171,994 + GFF5298 0.22 +0.1 171,994 + GFF5298 0.22 +0.1 171,994 + GFF5298 0.22 -0.0 171,995 - GFF5298 0.22 -1.4 171,995 - GFF5298 0.22 -0.6 172,072 + GFF5298 0.25 -0.0 172,072 + GFF5298 0.25 +0.3 172,073 - GFF5298 0.25 +0.3 172,073 - GFF5298 0.25 -1.1 172,120 + GFF5298 0.27 +0.3 172,120 + GFF5298 0.27 -0.0 172,121 - GFF5298 0.27 +1.7 172,121 - GFF5298 0.27 -1.2 172,121 - GFF5298 0.27 +0.6 172,121 - GFF5298 0.27 +1.1 172,121 - GFF5298 0.27 -1.0
Or see this region's nucleotide sequence