Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5291 and GFF5292 are separated by 6 nucleotides GFF5292 and GFF5293 are separated by 23 nucleotides GFF5293 and GFF5294 are separated by 6 nucleotides
GFF5291 - Ferrichrome-iron receptor, at 162,118 to 164,268
GFF5291
GFF5292 - no description, at 164,275 to 164,778
GFF5292
GFF5293 - N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87), at 164,802 to 166,052
GFF5293
GFF5294 - Cardiolipin synthase (EC 2.7.8.-) phosphatidylethanolamine-utilizing, bacterial type ClsC, at 166,059 to 167,648
GFF5294
Position (kb) 164
165
166
167 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 163.873 kb on + strand, within GFF5291 at 163.873 kb on + strand, within GFF5291 at 163.873 kb on + strand, within GFF5291 at 163.879 kb on + strand, within GFF5291 at 163.879 kb on + strand, within GFF5291 at 163.879 kb on + strand, within GFF5291 at 163.879 kb on + strand, within GFF5291 at 163.880 kb on - strand, within GFF5291 at 163.880 kb on - strand, within GFF5291 at 163.880 kb on - strand, within GFF5291 at 163.979 kb on - strand, within GFF5291 at 163.979 kb on - strand, within GFF5291 at 164.035 kb on + strand, within GFF5291 at 164.035 kb on + strand, within GFF5291 at 164.035 kb on + strand, within GFF5291 at 164.035 kb on + strand, within GFF5291 at 164.035 kb on + strand, within GFF5291 at 164.036 kb on - strand, within GFF5291 at 164.036 kb on - strand, within GFF5291 at 164.098 kb on + strand at 164.185 kb on + strand at 164.311 kb on + strand at 164.311 kb on + strand at 164.312 kb on - strand at 164.312 kb on - strand at 164.458 kb on + strand, within GFF5292 at 164.459 kb on - strand, within GFF5292 at 164.518 kb on + strand, within GFF5292 at 164.518 kb on + strand, within GFF5292 at 164.518 kb on + strand, within GFF5292 at 164.518 kb on + strand, within GFF5292 at 164.519 kb on - strand, within GFF5292 at 164.519 kb on - strand, within GFF5292 at 164.584 kb on + strand, within GFF5292 at 164.584 kb on + strand, within GFF5292 at 164.584 kb on + strand, within GFF5292 at 164.972 kb on + strand, within GFF5293 at 164.972 kb on + strand, within GFF5293 at 165.594 kb on + strand, within GFF5293 at 165.594 kb on + strand, within GFF5293 at 165.594 kb on + strand, within GFF5293 at 165.594 kb on + strand, within GFF5293 at 165.595 kb on - strand, within GFF5293 at 165.595 kb on - strand, within GFF5293 at 166.060 kb on + strand at 166.060 kb on + strand at 166.061 kb on - strand at 166.240 kb on + strand, within GFF5294 at 166.240 kb on + strand, within GFF5294 at 166.453 kb on + strand, within GFF5294 at 166.453 kb on + strand, within GFF5294 at 166.454 kb on - strand, within GFF5294 at 166.454 kb on - strand, within GFF5294 at 166.454 kb on - strand, within GFF5294 at 166.489 kb on + strand, within GFF5294 at 166.498 kb on + strand, within GFF5294 at 166.499 kb on - strand, within GFF5294 at 166.499 kb on - strand, within GFF5294 at 166.499 kb on - strand, within GFF5294 at 166.499 kb on - strand, within GFF5294 at 166.499 kb on - strand, within GFF5294 at 166.618 kb on + strand, within GFF5294 at 166.618 kb on + strand, within GFF5294 at 166.618 kb on + strand, within GFF5294 at 166.619 kb on - strand, within GFF5294 at 166.619 kb on - strand, within GFF5294 at 166.619 kb on - strand, within GFF5294 at 166.951 kb on + strand, within GFF5294 at 166.951 kb on + strand, within GFF5294 at 166.952 kb on - strand, within GFF5294 at 166.952 kb on - strand, within GFF5294 at 166.952 kb on - strand, within GFF5294 at 166.952 kb on - strand, within GFF5294
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 163,873 + GFF5291 0.82 -0.6 163,873 + GFF5291 0.82 -0.5 163,873 + GFF5291 0.82 -0.3 163,879 + GFF5291 0.82 +0.3 163,879 + GFF5291 0.82 +0.3 163,879 + GFF5291 0.82 +1.1 163,879 + GFF5291 0.82 +0.1 163,880 - GFF5291 0.82 -0.7 163,880 - GFF5291 0.82 -0.4 163,880 - GFF5291 0.82 -0.7 163,979 - GFF5291 0.87 -0.2 163,979 - GFF5291 0.87 +0.0 164,035 + GFF5291 0.89 +0.3 164,035 + GFF5291 0.89 +1.3 164,035 + GFF5291 0.89 -1.2 164,035 + GFF5291 0.89 +1.0 164,035 + GFF5291 0.89 +2.1 164,036 - GFF5291 0.89 -0.9 164,036 - GFF5291 0.89 -0.9 164,098 + +0.3 164,185 + -1.5 164,311 + +0.0 164,311 + -1.7 164,312 - -2.0 164,312 - -1.0 164,458 + GFF5292 0.36 -2.1 164,459 - GFF5292 0.37 -1.6 164,518 + GFF5292 0.48 +0.3 164,518 + GFF5292 0.48 -0.5 164,518 + GFF5292 0.48 +0.1 164,518 + GFF5292 0.48 -0.6 164,519 - GFF5292 0.48 -1.4 164,519 - GFF5292 0.48 +0.4 164,584 + GFF5292 0.61 +1.1 164,584 + GFF5292 0.61 -0.2 164,584 + GFF5292 0.61 +0.9 164,972 + GFF5293 0.14 -1.0 164,972 + GFF5293 0.14 +1.1 165,594 + GFF5293 0.63 +0.1 165,594 + GFF5293 0.63 +3.3 165,594 + GFF5293 0.63 +1.1 165,594 + GFF5293 0.63 +0.1 165,595 - GFF5293 0.63 +0.1 165,595 - GFF5293 0.63 +0.2 166,060 + +0.4 166,060 + -0.1 166,061 - -0.7 166,240 + GFF5294 0.11 -0.2 166,240 + GFF5294 0.11 -0.2 166,453 + GFF5294 0.25 -0.7 166,453 + GFF5294 0.25 +1.3 166,454 - GFF5294 0.25 -0.0 166,454 - GFF5294 0.25 -1.2 166,454 - GFF5294 0.25 -2.1 166,489 + GFF5294 0.27 +0.2 166,498 + GFF5294 0.28 +0.3 166,499 - GFF5294 0.28 +0.1 166,499 - GFF5294 0.28 -0.1 166,499 - GFF5294 0.28 -0.5 166,499 - GFF5294 0.28 -0.2 166,499 - GFF5294 0.28 -0.7 166,618 + GFF5294 0.35 -0.8 166,618 + GFF5294 0.35 -0.6 166,618 + GFF5294 0.35 -0.4 166,619 - GFF5294 0.35 -0.7 166,619 - GFF5294 0.35 -0.6 166,619 - GFF5294 0.35 +0.6 166,951 + GFF5294 0.56 -0.3 166,951 + GFF5294 0.56 +0.2 166,952 - GFF5294 0.56 -0.6 166,952 - GFF5294 0.56 -0.3 166,952 - GFF5294 0.56 -0.9 166,952 - GFF5294 0.56 -0.4
Or see this region's nucleotide sequence