Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5170 and GFF5171 are separated by 8 nucleotides GFF5171 and GFF5172 are separated by 138 nucleotides GFF5172 and GFF5173 are separated by 16 nucleotides GFF5173 and GFF5174 are separated by 49 nucleotides GFF5174 and GFF5175 are separated by 9 nucleotides
GFF5170 - N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157), at 22,279 to 23,706
GFF5170
GFF5171 - Transcriptional regulator, AsnC family, at 23,715 to 24,206
GFF5171
GFF5172 - Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16), at 24,345 to 26,225
GFF5172
GFF5173 - Hypothetical protein DUF454, at 26,242 to 26,658
GFF5173
GFF5174 - Uncharacterized conserved protein, at 26,708 to 27,100
GFF5174
GFF5175 - Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.5.2) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88), at 27,110 to 30,844
GFF5175
Position (kb) 24
25
26
27 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 24.226 kb on + strand at 24.307 kb on + strand at 24.307 kb on + strand at 24.308 kb on - strand at 26.293 kb on - strand, within GFF5173 at 26.729 kb on + strand at 26.730 kb on - strand at 26.816 kb on + strand, within GFF5174 at 26.816 kb on + strand, within GFF5174 at 26.816 kb on + strand, within GFF5174 at 26.816 kb on + strand, within GFF5174 at 26.816 kb on + strand, within GFF5174 at 26.816 kb on + strand, within GFF5174 at 26.817 kb on - strand, within GFF5174 at 26.817 kb on - strand, within GFF5174 at 26.817 kb on - strand, within GFF5174 at 26.817 kb on - strand, within GFF5174 at 26.817 kb on - strand, within GFF5174 at 26.817 kb on - strand, within GFF5174 at 26.915 kb on + strand, within GFF5174 at 26.915 kb on + strand, within GFF5174 at 26.915 kb on + strand, within GFF5174 at 26.915 kb on + strand, within GFF5174 at 26.915 kb on + strand, within GFF5174 at 26.915 kb on + strand, within GFF5174 at 26.916 kb on - strand, within GFF5174 at 26.916 kb on - strand, within GFF5174 at 26.916 kb on - strand, within GFF5174 at 26.916 kb on - strand, within GFF5174 at 26.916 kb on - strand, within GFF5174 at 26.916 kb on - strand, within GFF5174 at 26.916 kb on - strand, within GFF5174 at 26.916 kb on - strand, within GFF5174 at 26.925 kb on + strand, within GFF5174 at 26.925 kb on + strand, within GFF5174 at 26.926 kb on - strand, within GFF5174 at 26.926 kb on - strand, within GFF5174 at 26.949 kb on - strand, within GFF5174 at 26.949 kb on - strand, within GFF5174 at 27.065 kb on + strand at 27.098 kb on + strand at 27.098 kb on + strand at 27.099 kb on - strand at 27.099 kb on - strand at 27.099 kb on - strand at 27.099 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 24,226 + +1.3 24,307 + -0.6 24,307 + -0.4 24,308 - +0.3 26,293 - GFF5173 0.12 +1.0 26,729 + +0.1 26,730 - -1.7 26,816 + GFF5174 0.27 -0.5 26,816 + GFF5174 0.27 -0.2 26,816 + GFF5174 0.27 +0.6 26,816 + GFF5174 0.27 -0.5 26,816 + GFF5174 0.27 +0.3 26,816 + GFF5174 0.27 -0.1 26,817 - GFF5174 0.28 -2.4 26,817 - GFF5174 0.28 -0.6 26,817 - GFF5174 0.28 -0.4 26,817 - GFF5174 0.28 -0.6 26,817 - GFF5174 0.28 +0.8 26,817 - GFF5174 0.28 -2.8 26,915 + GFF5174 0.53 -0.2 26,915 + GFF5174 0.53 -0.8 26,915 + GFF5174 0.53 -2.1 26,915 + GFF5174 0.53 -0.0 26,915 + GFF5174 0.53 -0.7 26,915 + GFF5174 0.53 +0.7 26,916 - GFF5174 0.53 +0.1 26,916 - GFF5174 0.53 -0.0 26,916 - GFF5174 0.53 +2.1 26,916 - GFF5174 0.53 +0.1 26,916 - GFF5174 0.53 +0.2 26,916 - GFF5174 0.53 -0.0 26,916 - GFF5174 0.53 -0.1 26,916 - GFF5174 0.53 -0.9 26,925 + GFF5174 0.55 -1.4 26,925 + GFF5174 0.55 -0.8 26,926 - GFF5174 0.55 -1.7 26,926 - GFF5174 0.55 -0.0 26,949 - GFF5174 0.61 -0.0 26,949 - GFF5174 0.61 -0.9 27,065 + -1.4 27,098 + -1.1 27,098 + -1.9 27,099 - +0.6 27,099 - -0.6 27,099 - -0.5 27,099 - +0.6
Or see this region's nucleotide sequence