Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5150 and GFF5151 are separated by 100 nucleotides GFF5151 and GFF5152 are separated by 10 nucleotides GFF5152 and GFF5153 are separated by 34 nucleotides GFF5153 and GFF5154 overlap by 28 nucleotides
GFF5150 - Taurine ABC transporter, permease protein TauC, at 3,286 to 4,155
GFF5150
GFF5151 - Histidinol-phosphatase [alternative form] (EC 3.1.3.15), at 4,256 to 5,032
GFF5151
GFF5152 - Uncharacterized membrane-anchored protein Rmet_0180, at 5,043 to 5,237
GFF5152
GFF5153 - Putative ROK-family transcriptional regulator, at 5,272 to 6,198
GFF5153
GFF5154 - DNA-binding transcriptional regulator, MocR family / aminotransferase domain, at 6,171 to 7,712
GFF5154
Position (kb) 5
6 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4.125 kb on + strand at 4.125 kb on + strand at 4.125 kb on + strand at 4.125 kb on + strand at 4.125 kb on + strand at 4.125 kb on + strand at 4.130 kb on + strand at 4.220 kb on + strand at 4.220 kb on + strand at 4.221 kb on - strand at 5.031 kb on - strand at 5.235 kb on + strand at 5.235 kb on + strand at 5.236 kb on - strand at 5.285 kb on + strand at 5.286 kb on - strand at 5.286 kb on - strand at 5.286 kb on - strand at 5.707 kb on + strand, within GFF5153 at 5.707 kb on + strand, within GFF5153 at 5.708 kb on - strand, within GFF5153 at 5.708 kb on - strand, within GFF5153 at 5.848 kb on + strand, within GFF5153 at 5.848 kb on + strand, within GFF5153 at 5.848 kb on + strand, within GFF5153 at 5.848 kb on + strand, within GFF5153 at 5.849 kb on - strand, within GFF5153 at 5.849 kb on - strand, within GFF5153 at 5.858 kb on + strand, within GFF5153 at 5.859 kb on - strand, within GFF5153 at 5.859 kb on - strand, within GFF5153 at 5.859 kb on - strand, within GFF5153 at 5.859 kb on - strand, within GFF5153 at 5.859 kb on - strand, within GFF5153 at 5.998 kb on + strand, within GFF5153 at 5.998 kb on + strand, within GFF5153 at 5.998 kb on + strand, within GFF5153 at 5.998 kb on + strand, within GFF5153 at 5.998 kb on + strand, within GFF5153 at 5.998 kb on + strand, within GFF5153 at 5.998 kb on + strand, within GFF5153 at 5.998 kb on + strand, within GFF5153 at 5.998 kb on + strand, within GFF5153 at 5.998 kb on + strand, within GFF5153 at 5.998 kb on + strand, within GFF5153 at 5.998 kb on + strand, within GFF5153 at 5.998 kb on + strand, within GFF5153 at 5.998 kb on + strand, within GFF5153 at 5.999 kb on - strand, within GFF5153 at 5.999 kb on - strand, within GFF5153 at 5.999 kb on - strand, within GFF5153 at 5.999 kb on - strand, within GFF5153 at 5.999 kb on - strand, within GFF5153 at 5.999 kb on - strand, within GFF5153 at 5.999 kb on - strand, within GFF5153 at 6.172 kb on + strand at 6.173 kb on - strand at 6.173 kb on - strand at 6.173 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 4,125 + -0.3 4,125 + -0.3 4,125 + +0.1 4,125 + +1.7 4,125 + -1.0 4,125 + +0.4 4,130 + -0.1 4,220 + +3.7 4,220 + +0.2 4,221 - -0.8 5,031 - -0.6 5,235 + -0.8 5,235 + -0.6 5,236 - -0.5 5,285 + -1.6 5,286 - -0.9 5,286 - -1.3 5,286 - -0.3 5,707 + GFF5153 0.47 +0.1 5,707 + GFF5153 0.47 -1.3 5,708 - GFF5153 0.47 -0.2 5,708 - GFF5153 0.47 -2.0 5,848 + GFF5153 0.62 +1.5 5,848 + GFF5153 0.62 -1.0 5,848 + GFF5153 0.62 +0.3 5,848 + GFF5153 0.62 +0.1 5,849 - GFF5153 0.62 -0.2 5,849 - GFF5153 0.62 +0.6 5,858 + GFF5153 0.63 -1.4 5,859 - GFF5153 0.63 -0.2 5,859 - GFF5153 0.63 +0.1 5,859 - GFF5153 0.63 -2.3 5,859 - GFF5153 0.63 -1.1 5,859 - GFF5153 0.63 -1.8 5,998 + GFF5153 0.78 -1.5 5,998 + GFF5153 0.78 -1.9 5,998 + GFF5153 0.78 -0.9 5,998 + GFF5153 0.78 +0.6 5,998 + GFF5153 0.78 -1.4 5,998 + GFF5153 0.78 -0.7 5,998 + GFF5153 0.78 -0.4 5,998 + GFF5153 0.78 -0.7 5,998 + GFF5153 0.78 -0.9 5,998 + GFF5153 0.78 -1.9 5,998 + GFF5153 0.78 -1.0 5,998 + GFF5153 0.78 -1.0 5,998 + GFF5153 0.78 +2.1 5,998 + GFF5153 0.78 -0.5 5,999 - GFF5153 0.78 +1.1 5,999 - GFF5153 0.78 +0.6 5,999 - GFF5153 0.78 -0.9 5,999 - GFF5153 0.78 -1.6 5,999 - GFF5153 0.78 -0.3 5,999 - GFF5153 0.78 -0.8 5,999 - GFF5153 0.78 +0.1 6,172 + -0.9 6,173 - -1.6 6,173 - +0.6 6,173 - -2.2
Or see this region's nucleotide sequence