Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4841 and GFF4842 are separated by 2 nucleotides GFF4842 and GFF4843 overlap by 4 nucleotides GFF4843 and GFF4844 are separated by 18 nucleotides
GFF4841 - Conjugative transfer protein TrbI, at 210,531 to 211,802
GFF4841
GFF4842 - Conjugative transfer protein TrbG, at 211,805 to 212,800
GFF4842
GFF4843 - Conjugative transfer protein TrbF, at 212,797 to 213,501
GFF4843
GFF4844 - Conjugative transfer protein TrbL, at 213,520 to 214,872
GFF4844
Position (kb)
212
213
214 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 211.908 kb on + strand, within GFF4842 at 211.908 kb on + strand, within GFF4842 at 211.908 kb on + strand, within GFF4842 at 211.908 kb on + strand, within GFF4842 at 211.908 kb on + strand, within GFF4842 at 211.908 kb on + strand, within GFF4842 at 211.908 kb on + strand, within GFF4842 at 211.908 kb on + strand, within GFF4842 at 211.908 kb on + strand, within GFF4842 at 211.909 kb on - strand, within GFF4842 at 211.909 kb on - strand, within GFF4842 at 211.909 kb on - strand, within GFF4842 at 211.909 kb on - strand, within GFF4842 at 211.909 kb on - strand, within GFF4842 at 211.909 kb on - strand, within GFF4842 at 211.909 kb on - strand, within GFF4842 at 211.911 kb on + strand, within GFF4842 at 211.911 kb on + strand, within GFF4842 at 211.912 kb on - strand, within GFF4842 at 211.929 kb on + strand, within GFF4842 at 211.930 kb on - strand, within GFF4842 at 211.930 kb on - strand, within GFF4842 at 211.930 kb on - strand, within GFF4842 at 212.014 kb on + strand, within GFF4842 at 212.014 kb on + strand, within GFF4842 at 212.015 kb on - strand, within GFF4842 at 212.015 kb on - strand, within GFF4842 at 212.016 kb on + strand, within GFF4842 at 212.016 kb on + strand, within GFF4842 at 212.016 kb on + strand, within GFF4842 at 212.016 kb on + strand, within GFF4842 at 212.016 kb on + strand, within GFF4842 at 212.016 kb on + strand, within GFF4842 at 212.016 kb on + strand, within GFF4842 at 212.016 kb on + strand, within GFF4842 at 212.016 kb on + strand, within GFF4842 at 212.016 kb on + strand, within GFF4842 at 212.016 kb on + strand, within GFF4842 at 212.017 kb on - strand, within GFF4842 at 212.017 kb on - strand, within GFF4842 at 212.017 kb on - strand, within GFF4842 at 212.017 kb on - strand, within GFF4842 at 212.017 kb on - strand, within GFF4842 at 212.017 kb on - strand, within GFF4842 at 212.184 kb on + strand, within GFF4842 at 212.184 kb on + strand, within GFF4842 at 212.185 kb on - strand, within GFF4842 at 212.241 kb on + strand, within GFF4842 at 212.241 kb on + strand, within GFF4842 at 212.241 kb on + strand, within GFF4842 at 212.241 kb on + strand, within GFF4842 at 212.242 kb on - strand, within GFF4842 at 212.363 kb on + strand, within GFF4842 at 212.364 kb on - strand, within GFF4842 at 212.432 kb on + strand, within GFF4842 at 212.442 kb on + strand, within GFF4842 at 212.442 kb on + strand, within GFF4842 at 212.443 kb on - strand, within GFF4842 at 212.443 kb on - strand, within GFF4842 at 212.451 kb on + strand, within GFF4842 at 212.451 kb on + strand, within GFF4842 at 212.452 kb on - strand, within GFF4842 at 212.496 kb on + strand, within GFF4842 at 212.496 kb on + strand, within GFF4842 at 212.496 kb on + strand, within GFF4842 at 212.497 kb on - strand, within GFF4842 at 212.497 kb on - strand, within GFF4842 at 212.682 kb on - strand, within GFF4842 at 212.682 kb on - strand, within GFF4842 at 212.783 kb on - strand at 212.855 kb on + strand at 212.855 kb on + strand at 212.855 kb on + strand at 212.855 kb on + strand at 212.855 kb on + strand at 212.855 kb on + strand at 212.855 kb on + strand at 212.856 kb on - strand at 212.856 kb on - strand at 212.856 kb on - strand at 212.856 kb on - strand at 212.856 kb on - strand at 213.081 kb on - strand, within GFF4843 at 213.117 kb on - strand, within GFF4843 at 213.122 kb on + strand, within GFF4843 at 213.122 kb on + strand, within GFF4843 at 213.123 kb on - strand, within GFF4843 at 213.291 kb on - strand, within GFF4843 at 213.291 kb on - strand, within GFF4843 at 213.388 kb on - strand, within GFF4843 at 213.388 kb on - strand, within GFF4843 at 213.388 kb on - strand, within GFF4843 at 213.388 kb on - strand, within GFF4843 at 213.473 kb on + strand at 213.600 kb on + strand at 213.600 kb on + strand at 213.771 kb on - strand, within GFF4844 at 213.771 kb on - strand, within GFF4844 at 214.183 kb on + strand, within GFF4844 at 214.183 kb on + strand, within GFF4844 at 214.183 kb on + strand, within GFF4844 at 214.183 kb on + strand, within GFF4844 at 214.184 kb on - strand, within GFF4844 at 214.184 kb on - strand, within GFF4844 at 214.285 kb on + strand, within GFF4844 at 214.286 kb on - strand, within GFF4844
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 211,908 + GFF4842 0.10 -1.9 211,908 + GFF4842 0.10 -1.9 211,908 + GFF4842 0.10 -0.7 211,908 + GFF4842 0.10 -1.7 211,908 + GFF4842 0.10 +1.3 211,908 + GFF4842 0.10 -0.5 211,908 + GFF4842 0.10 -0.5 211,908 + GFF4842 0.10 -0.3 211,908 + GFF4842 0.10 +0.7 211,909 - GFF4842 0.10 -0.2 211,909 - GFF4842 0.10 -1.1 211,909 - GFF4842 0.10 -0.4 211,909 - GFF4842 0.10 +0.3 211,909 - GFF4842 0.10 -1.1 211,909 - GFF4842 0.10 -0.1 211,909 - GFF4842 0.10 +0.2 211,911 + GFF4842 0.11 -1.2 211,911 + GFF4842 0.11 -0.1 211,912 - GFF4842 0.11 -0.2 211,929 + GFF4842 0.12 +1.0 211,930 - GFF4842 0.13 -1.1 211,930 - GFF4842 0.13 -0.5 211,930 - GFF4842 0.13 -0.2 212,014 + GFF4842 0.21 -0.8 212,014 + GFF4842 0.21 -0.4 212,015 - GFF4842 0.21 -1.1 212,015 - GFF4842 0.21 +0.3 212,016 + GFF4842 0.21 -0.5 212,016 + GFF4842 0.21 +0.3 212,016 + GFF4842 0.21 -1.4 212,016 + GFF4842 0.21 -1.2 212,016 + GFF4842 0.21 -0.8 212,016 + GFF4842 0.21 -1.0 212,016 + GFF4842 0.21 +0.5 212,016 + GFF4842 0.21 -2.0 212,016 + GFF4842 0.21 +1.9 212,016 + GFF4842 0.21 -0.8 212,016 + GFF4842 0.21 +0.4 212,017 - GFF4842 0.21 -0.7 212,017 - GFF4842 0.21 +0.3 212,017 - GFF4842 0.21 -0.7 212,017 - GFF4842 0.21 +0.1 212,017 - GFF4842 0.21 -1.1 212,017 - GFF4842 0.21 -0.6 212,184 + GFF4842 0.38 -1.2 212,184 + GFF4842 0.38 +0.4 212,185 - GFF4842 0.38 -0.5 212,241 + GFF4842 0.44 +2.0 212,241 + GFF4842 0.44 -0.6 212,241 + GFF4842 0.44 +0.9 212,241 + GFF4842 0.44 +0.1 212,242 - GFF4842 0.44 +1.1 212,363 + GFF4842 0.56 -0.5 212,364 - GFF4842 0.56 +0.9 212,432 + GFF4842 0.63 +0.6 212,442 + GFF4842 0.64 -0.5 212,442 + GFF4842 0.64 -0.3 212,443 - GFF4842 0.64 +0.2 212,443 - GFF4842 0.64 +0.0 212,451 + GFF4842 0.65 -1.5 212,451 + GFF4842 0.65 +0.1 212,452 - GFF4842 0.65 -1.6 212,496 + GFF4842 0.69 +1.1 212,496 + GFF4842 0.69 -0.6 212,496 + GFF4842 0.69 +1.5 212,497 - GFF4842 0.69 -0.1 212,497 - GFF4842 0.69 -0.5 212,682 - GFF4842 0.88 -1.6 212,682 - GFF4842 0.88 -0.0 212,783 - -1.1 212,855 + +0.6 212,855 + -0.7 212,855 + -0.2 212,855 + -0.4 212,855 + -3.5 212,855 + +1.1 212,855 + -0.7 212,856 - +0.7 212,856 - -0.1 212,856 - -2.0 212,856 - -0.1 212,856 - -0.1 213,081 - GFF4843 0.40 +1.9 213,117 - GFF4843 0.45 -1.0 213,122 + GFF4843 0.46 -0.3 213,122 + GFF4843 0.46 -0.1 213,123 - GFF4843 0.46 -0.3 213,291 - GFF4843 0.70 +0.9 213,291 - GFF4843 0.70 -0.5 213,388 - GFF4843 0.84 -1.4 213,388 - GFF4843 0.84 -0.1 213,388 - GFF4843 0.84 -0.1 213,388 - GFF4843 0.84 -0.1 213,473 + -0.3 213,600 + +0.3 213,600 + -0.1 213,771 - GFF4844 0.19 +0.3 213,771 - GFF4844 0.19 +0.1 214,183 + GFF4844 0.49 -0.8 214,183 + GFF4844 0.49 -0.7 214,183 + GFF4844 0.49 -0.3 214,183 + GFF4844 0.49 -0.2 214,184 - GFF4844 0.49 +0.2 214,184 - GFF4844 0.49 -0.4 214,285 + GFF4844 0.57 +1.0 214,286 - GFF4844 0.57 -1.1
Or see this region's nucleotide sequence