Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4710 and GFF4711 overlap by 1 nucleotides GFF4711 and GFF4712 are separated by 109 nucleotides GFF4712 and GFF4713 are separated by 111 nucleotides GFF4713 and GFF4714 are separated by 11 nucleotides GFF4714 and GFF4715 overlap by 1 nucleotides
GFF4710 - MutT/Nudix family protein, at 73,904 to 74,380
GFF4710
GFF4711 - Transamidase GatB domain protein, at 74,380 to 74,829
GFF4711
GFF4712 - SSU ribosomal protein S21p, at 74,939 to 75,151
GFF4712
GFF4713 - Uncharacterized protein YhiN, at 75,263 to 76,468
GFF4713
GFF4714 - putative lipoprotein, at 76,480 to 77,271
GFF4714
GFF4715 - Fatty acid hydroxylase family (carotene hydroxylase/sterol desaturase), at 77,271 to 78,401
GFF4715
Position (kb) 75
76
77 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 74.564 kb on + strand, within GFF4711 at 74.564 kb on + strand, within GFF4711 at 74.564 kb on + strand, within GFF4711 at 74.564 kb on + strand, within GFF4711 at 74.564 kb on + strand, within GFF4711 at 74.564 kb on + strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.565 kb on - strand, within GFF4711 at 74.940 kb on + strand at 74.940 kb on + strand at 74.940 kb on + strand at 74.940 kb on + strand at 74.940 kb on + strand at 74.940 kb on + strand at 74.940 kb on + strand at 74.940 kb on + strand at 74.940 kb on + strand at 74.940 kb on + strand at 74.940 kb on + strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.941 kb on - strand at 74.943 kb on + strand at 74.943 kb on + strand at 74.943 kb on + strand at 74.943 kb on + strand at 74.943 kb on + strand at 74.943 kb on + strand at 74.943 kb on + strand at 74.944 kb on - strand at 74.944 kb on - strand at 74.944 kb on - strand at 74.944 kb on - strand at 74.944 kb on - strand at 74.944 kb on - strand at 74.944 kb on - strand at 74.944 kb on - strand at 75.266 kb on + strand at 75.266 kb on + strand at 75.266 kb on + strand at 75.266 kb on + strand at 75.267 kb on - strand at 75.267 kb on - strand at 75.313 kb on + strand at 75.313 kb on + strand at 75.313 kb on + strand at 75.313 kb on + strand at 75.313 kb on + strand at 75.313 kb on + strand at 75.313 kb on + strand at 75.313 kb on + strand at 75.313 kb on + strand at 75.314 kb on - strand at 75.314 kb on - strand at 75.314 kb on - strand at 75.314 kb on - strand at 75.314 kb on - strand at 75.314 kb on - strand at 75.314 kb on - strand at 75.314 kb on - strand at 75.314 kb on - strand at 75.314 kb on - strand at 75.315 kb on + strand at 75.315 kb on + strand at 75.315 kb on + strand at 75.315 kb on + strand at 75.315 kb on + strand at 75.344 kb on + strand at 75.344 kb on + strand at 75.444 kb on + strand, within GFF4713 at 75.444 kb on + strand, within GFF4713 at 75.444 kb on + strand, within GFF4713 at 75.445 kb on - strand, within GFF4713 at 75.445 kb on - strand, within GFF4713 at 75.445 kb on - strand, within GFF4713 at 75.706 kb on - strand, within GFF4713 at 75.706 kb on - strand, within GFF4713 at 75.706 kb on - strand, within GFF4713 at 75.706 kb on - strand, within GFF4713 at 75.706 kb on - strand, within GFF4713 at 75.841 kb on - strand, within GFF4713 at 75.841 kb on - strand, within GFF4713 at 75.876 kb on - strand, within GFF4713 at 75.876 kb on - strand, within GFF4713 at 76.029 kb on + strand, within GFF4713 at 76.053 kb on - strand, within GFF4713 at 76.053 kb on - strand, within GFF4713 at 76.225 kb on - strand, within GFF4713 at 76.517 kb on + strand at 76.517 kb on + strand at 76.517 kb on + strand at 76.517 kb on + strand at 76.517 kb on + strand at 76.517 kb on + strand at 76.517 kb on + strand at 76.517 kb on + strand at 76.518 kb on - strand at 76.518 kb on - strand at 76.518 kb on - strand at 76.535 kb on + strand at 76.535 kb on + strand at 76.535 kb on + strand at 76.536 kb on - strand at 76.536 kb on - strand at 76.536 kb on - strand at 76.653 kb on - strand, within GFF4714 at 76.653 kb on - strand, within GFF4714 at 76.653 kb on - strand, within GFF4714 at 76.736 kb on + strand, within GFF4714 at 76.736 kb on + strand, within GFF4714 at 76.737 kb on - strand, within GFF4714 at 76.737 kb on - strand, within GFF4714 at 76.737 kb on - strand, within GFF4714 at 76.737 kb on - strand, within GFF4714 at 76.737 kb on - strand, within GFF4714 at 76.826 kb on + strand, within GFF4714 at 76.826 kb on + strand, within GFF4714 at 76.827 kb on - strand, within GFF4714 at 76.827 kb on - strand, within GFF4714 at 76.827 kb on - strand, within GFF4714 at 76.827 kb on - strand, within GFF4714 at 76.827 kb on - strand, within GFF4714 at 76.997 kb on + strand, within GFF4714 at 76.997 kb on + strand, within GFF4714 at 76.997 kb on + strand, within GFF4714 at 76.998 kb on - strand, within GFF4714 at 76.998 kb on - strand, within GFF4714 at 76.998 kb on - strand, within GFF4714 at 76.998 kb on - strand, within GFF4714 at 76.998 kb on - strand, within GFF4714 at 77.212 kb on + strand at 77.212 kb on + strand at 77.213 kb on - strand at 77.326 kb on + strand at 77.327 kb on - strand at 77.327 kb on - strand at 77.327 kb on - strand at 77.344 kb on + strand at 77.344 kb on + strand at 77.344 kb on + strand at 77.345 kb on - strand at 77.345 kb on - strand at 77.345 kb on - strand at 77.345 kb on - strand at 77.345 kb on - strand at 77.392 kb on + strand, within GFF4715 at 77.392 kb on + strand, within GFF4715 at 77.392 kb on + strand, within GFF4715 at 77.392 kb on + strand, within GFF4715 at 77.392 kb on + strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.407 kb on + strand, within GFF4715 at 77.407 kb on + strand, within GFF4715 at 77.407 kb on + strand, within GFF4715 at 77.407 kb on + strand, within GFF4715 at 77.407 kb on + strand, within GFF4715 at 77.408 kb on - strand, within GFF4715 at 77.419 kb on + strand, within GFF4715 at 77.419 kb on + strand, within GFF4715 at 77.419 kb on + strand, within GFF4715 at 77.419 kb on + strand, within GFF4715 at 77.420 kb on - strand, within GFF4715 at 77.420 kb on - strand, within GFF4715 at 77.420 kb on - strand, within GFF4715 at 77.420 kb on - strand, within GFF4715 at 77.420 kb on - strand, within GFF4715 at 77.420 kb on - strand, within GFF4715
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 74,564 + GFF4711 0.41 -0.6 74,564 + GFF4711 0.41 -0.2 74,564 + GFF4711 0.41 -0.3 74,564 + GFF4711 0.41 +0.5 74,564 + GFF4711 0.41 +0.0 74,564 + GFF4711 0.41 +1.4 74,565 - GFF4711 0.41 -0.7 74,565 - GFF4711 0.41 -0.3 74,565 - GFF4711 0.41 -0.6 74,565 - GFF4711 0.41 -0.1 74,565 - GFF4711 0.41 -0.6 74,565 - GFF4711 0.41 +0.8 74,565 - GFF4711 0.41 +0.4 74,565 - GFF4711 0.41 -0.6 74,565 - GFF4711 0.41 -0.0 74,565 - GFF4711 0.41 -1.0 74,565 - GFF4711 0.41 -1.1 74,565 - GFF4711 0.41 +1.3 74,565 - GFF4711 0.41 -0.1 74,565 - GFF4711 0.41 -0.1 74,565 - GFF4711 0.41 +0.3 74,940 + +0.3 74,940 + -0.4 74,940 + -1.1 74,940 + +1.0 74,940 + -1.1 74,940 + -0.2 74,940 + +1.3 74,940 + -0.8 74,940 + -0.1 74,940 + +0.0 74,940 + -0.2 74,941 - -0.7 74,941 - -0.9 74,941 - -0.8 74,941 - -0.5 74,941 - -0.3 74,941 - -0.7 74,941 - +0.3 74,941 - +0.9 74,941 - -0.3 74,941 - +0.6 74,941 - -0.1 74,941 - -0.6 74,941 - +0.6 74,941 - -0.3 74,941 - +0.0 74,941 - -0.7 74,943 + -0.7 74,943 + -0.8 74,943 + -2.0 74,943 + +0.2 74,943 + -1.8 74,943 + +0.6 74,943 + -0.6 74,944 - +1.2 74,944 - -0.7 74,944 - -2.2 74,944 - -0.5 74,944 - +0.8 74,944 - +0.9 74,944 - -0.3 74,944 - +0.9 75,266 + -1.0 75,266 + -0.2 75,266 + -1.2 75,266 + -0.7 75,267 - -0.9 75,267 - -0.2 75,313 + -0.1 75,313 + -0.5 75,313 + -0.8 75,313 + -1.6 75,313 + +0.5 75,313 + -1.0 75,313 + -0.5 75,313 + -0.5 75,313 + +0.1 75,314 - +0.1 75,314 - -0.7 75,314 - +0.5 75,314 - -0.8 75,314 - -0.4 75,314 - +1.1 75,314 - +0.3 75,314 - -1.0 75,314 - -0.2 75,314 - -0.8 75,315 + +0.3 75,315 + +0.1 75,315 + +0.5 75,315 + +2.3 75,315 + -1.6 75,344 + +1.0 75,344 + +1.0 75,444 + GFF4713 0.15 -0.6 75,444 + GFF4713 0.15 -0.3 75,444 + GFF4713 0.15 +0.3 75,445 - GFF4713 0.15 -1.2 75,445 - GFF4713 0.15 +0.6 75,445 - GFF4713 0.15 -0.3 75,706 - GFF4713 0.37 +0.6 75,706 - GFF4713 0.37 +0.8 75,706 - GFF4713 0.37 -1.2 75,706 - GFF4713 0.37 +0.9 75,706 - GFF4713 0.37 -0.7 75,841 - GFF4713 0.48 -0.4 75,841 - GFF4713 0.48 +0.9 75,876 - GFF4713 0.51 +0.9 75,876 - GFF4713 0.51 +1.1 76,029 + GFF4713 0.64 -0.2 76,053 - GFF4713 0.66 +3.0 76,053 - GFF4713 0.66 +1.3 76,225 - GFF4713 0.80 -0.4 76,517 + +0.1 76,517 + -0.1 76,517 + +0.6 76,517 + +0.8 76,517 + +0.5 76,517 + -0.9 76,517 + +0.2 76,517 + -0.3 76,518 - -0.6 76,518 - +0.4 76,518 - -1.7 76,535 + -0.6 76,535 + -1.1 76,535 + -0.2 76,536 - -1.2 76,536 - -0.1 76,536 - -1.4 76,653 - GFF4714 0.22 +0.3 76,653 - GFF4714 0.22 -0.2 76,653 - GFF4714 0.22 +0.4 76,736 + GFF4714 0.32 +0.3 76,736 + GFF4714 0.32 -0.1 76,737 - GFF4714 0.32 -1.5 76,737 - GFF4714 0.32 -1.2 76,737 - GFF4714 0.32 +0.2 76,737 - GFF4714 0.32 -1.4 76,737 - GFF4714 0.32 -0.5 76,826 + GFF4714 0.44 -1.5 76,826 + GFF4714 0.44 +0.9 76,827 - GFF4714 0.44 -0.0 76,827 - GFF4714 0.44 -0.1 76,827 - GFF4714 0.44 -0.0 76,827 - GFF4714 0.44 -0.1 76,827 - GFF4714 0.44 +0.6 76,997 + GFF4714 0.65 -2.0 76,997 + GFF4714 0.65 -1.3 76,997 + GFF4714 0.65 -0.7 76,998 - GFF4714 0.65 -0.0 76,998 - GFF4714 0.65 -1.0 76,998 - GFF4714 0.65 +0.8 76,998 - GFF4714 0.65 -0.8 76,998 - GFF4714 0.65 -1.3 77,212 + -0.8 77,212 + +1.2 77,213 - +0.7 77,326 + -0.6 77,327 - -0.4 77,327 - -1.5 77,327 - -0.8 77,344 + -2.9 77,344 + -0.2 77,344 + -0.6 77,345 - +0.2 77,345 - +2.9 77,345 - -0.4 77,345 - +0.0 77,345 - +0.3 77,392 + GFF4715 0.11 +0.1 77,392 + GFF4715 0.11 +0.1 77,392 + GFF4715 0.11 +1.2 77,392 + GFF4715 0.11 -0.6 77,392 + GFF4715 0.11 +0.4 77,393 - GFF4715 0.11 -0.9 77,393 - GFF4715 0.11 -1.5 77,393 - GFF4715 0.11 -0.3 77,393 - GFF4715 0.11 +0.5 77,393 - GFF4715 0.11 -1.8 77,393 - GFF4715 0.11 -1.0 77,393 - GFF4715 0.11 +0.1 77,393 - GFF4715 0.11 +1.6 77,393 - GFF4715 0.11 +0.9 77,393 - GFF4715 0.11 +0.4 77,393 - GFF4715 0.11 -0.4 77,407 + GFF4715 0.12 -0.7 77,407 + GFF4715 0.12 -0.6 77,407 + GFF4715 0.12 -0.2 77,407 + GFF4715 0.12 -0.0 77,407 + GFF4715 0.12 -1.0 77,408 - GFF4715 0.12 -0.1 77,419 + GFF4715 0.13 +0.4 77,419 + GFF4715 0.13 -0.9 77,419 + GFF4715 0.13 +1.9 77,419 + GFF4715 0.13 +0.1 77,420 - GFF4715 0.13 -2.1 77,420 - GFF4715 0.13 -0.4 77,420 - GFF4715 0.13 -0.0 77,420 - GFF4715 0.13 +0.6 77,420 - GFF4715 0.13 +1.1 77,420 - GFF4715 0.13 -0.3
Or see this region's nucleotide sequence