Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4680 and GFF4681 are separated by 76 nucleotides GFF4681 and GFF4682 are separated by 28 nucleotides GFF4682 and GFF4683 are separated by 69 nucleotides
GFF4680 - tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase (EC 2.1.1.200), at 39,541 to 40,281
GFF4680
GFF4681 - Serine acetyltransferase (EC 2.3.1.30), at 40,358 to 41,131
GFF4681
GFF4682 - Transcriptional regulator, MerR family, at 41,160 to 41,630
GFF4682
GFF4683 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 41,700 to 44,015
GFF4683
Position (kb) 41
42 Strain fitness (log2 ratio) -2
-1
0
1
2 at 40.279 kb on + strand at 40.297 kb on - strand at 40.297 kb on - strand at 40.315 kb on - strand at 40.316 kb on + strand at 40.316 kb on + strand at 40.316 kb on + strand at 40.316 kb on + strand at 40.317 kb on - strand at 40.317 kb on - strand at 40.317 kb on - strand at 40.317 kb on - strand at 40.317 kb on - strand at 40.317 kb on - strand at 40.339 kb on - strand at 40.340 kb on + strand at 40.340 kb on + strand at 41.129 kb on + strand at 41.130 kb on - strand at 41.130 kb on - strand at 41.130 kb on - strand at 41.130 kb on - strand at 41.130 kb on - strand at 41.130 kb on - strand at 41.130 kb on - strand at 41.509 kb on - strand, within GFF4682 at 41.516 kb on - strand, within GFF4682 at 41.516 kb on - strand, within GFF4682 at 41.516 kb on - strand, within GFF4682 at 41.516 kb on - strand, within GFF4682 at 41.516 kb on - strand, within GFF4682 at 41.516 kb on - strand, within GFF4682 at 41.516 kb on - strand, within GFF4682 at 41.516 kb on - strand, within GFF4682 at 41.516 kb on - strand, within GFF4682 at 41.524 kb on + strand, within GFF4682 at 41.524 kb on + strand, within GFF4682 at 41.524 kb on + strand, within GFF4682 at 41.524 kb on + strand, within GFF4682 at 41.524 kb on + strand, within GFF4682 at 41.524 kb on + strand, within GFF4682 at 41.525 kb on - strand, within GFF4682 at 41.525 kb on - strand, within GFF4682 at 41.572 kb on + strand, within GFF4682 at 41.572 kb on + strand, within GFF4682 at 41.572 kb on + strand, within GFF4682 at 41.572 kb on + strand, within GFF4682 at 41.572 kb on + strand, within GFF4682 at 41.573 kb on - strand, within GFF4682 at 41.573 kb on - strand, within GFF4682 at 41.573 kb on - strand, within GFF4682 at 41.573 kb on - strand, within GFF4682 at 41.573 kb on - strand, within GFF4682 at 41.573 kb on - strand, within GFF4682 at 41.573 kb on - strand, within GFF4682 at 41.576 kb on - strand, within GFF4682 at 41.670 kb on + strand at 41.670 kb on + strand at 41.670 kb on + strand at 41.670 kb on + strand at 41.671 kb on - strand at 41.671 kb on - strand at 42.297 kb on + strand, within GFF4683 at 42.297 kb on + strand, within GFF4683 at 42.297 kb on + strand, within GFF4683 at 42.298 kb on - strand, within GFF4683 at 42.298 kb on - strand, within GFF4683 at 42.298 kb on - strand, within GFF4683 at 42.298 kb on - strand, within GFF4683 at 42.298 kb on - strand, within GFF4683 at 42.298 kb on - strand, within GFF4683 at 42.298 kb on - strand, within GFF4683 at 42.298 kb on - strand, within GFF4683 at 42.298 kb on - strand, within GFF4683 at 42.298 kb on - strand, within GFF4683 at 42.298 kb on - strand, within GFF4683 at 42.420 kb on + strand, within GFF4683 at 42.420 kb on + strand, within GFF4683 at 42.420 kb on + strand, within GFF4683 at 42.421 kb on - strand, within GFF4683 at 42.421 kb on - strand, within GFF4683
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 40,279 + -2.5 40,297 - +1.5 40,297 - -0.5 40,315 - -0.5 40,316 + -1.1 40,316 + -1.0 40,316 + -0.1 40,316 + -0.7 40,317 - +0.4 40,317 - +1.5 40,317 - -0.3 40,317 - +1.3 40,317 - -0.1 40,317 - -1.2 40,339 - +1.4 40,340 + -0.3 40,340 + -0.0 41,129 + -0.6 41,130 - +0.1 41,130 - -0.1 41,130 - +0.1 41,130 - +0.1 41,130 - -1.5 41,130 - +1.6 41,130 - +1.1 41,509 - GFF4682 0.74 +0.1 41,516 - GFF4682 0.76 -0.1 41,516 - GFF4682 0.76 +0.4 41,516 - GFF4682 0.76 +0.2 41,516 - GFF4682 0.76 -0.7 41,516 - GFF4682 0.76 -1.1 41,516 - GFF4682 0.76 -0.7 41,516 - GFF4682 0.76 -0.5 41,516 - GFF4682 0.76 +1.0 41,516 - GFF4682 0.76 -1.1 41,524 + GFF4682 0.77 -0.3 41,524 + GFF4682 0.77 -0.3 41,524 + GFF4682 0.77 -0.3 41,524 + GFF4682 0.77 +0.2 41,524 + GFF4682 0.77 -0.1 41,524 + GFF4682 0.77 +0.0 41,525 - GFF4682 0.77 -0.9 41,525 - GFF4682 0.77 +0.1 41,572 + GFF4682 0.87 -0.0 41,572 + GFF4682 0.87 -0.1 41,572 + GFF4682 0.87 +0.3 41,572 + GFF4682 0.87 -0.0 41,572 + GFF4682 0.87 -0.1 41,573 - GFF4682 0.88 -2.1 41,573 - GFF4682 0.88 +0.9 41,573 - GFF4682 0.88 +1.9 41,573 - GFF4682 0.88 +0.2 41,573 - GFF4682 0.88 +1.3 41,573 - GFF4682 0.88 +0.0 41,573 - GFF4682 0.88 -0.1 41,576 - GFF4682 0.88 +0.3 41,670 + -1.7 41,670 + +0.3 41,670 + +1.2 41,670 + +0.9 41,671 - +1.0 41,671 - -1.5 42,297 + GFF4683 0.26 +0.1 42,297 + GFF4683 0.26 -0.4 42,297 + GFF4683 0.26 -0.1 42,298 - GFF4683 0.26 -0.6 42,298 - GFF4683 0.26 +0.2 42,298 - GFF4683 0.26 -0.7 42,298 - GFF4683 0.26 +1.0 42,298 - GFF4683 0.26 +0.5 42,298 - GFF4683 0.26 -1.3 42,298 - GFF4683 0.26 -0.1 42,298 - GFF4683 0.26 -0.9 42,298 - GFF4683 0.26 -0.3 42,298 - GFF4683 0.26 -1.2 42,298 - GFF4683 0.26 +0.4 42,420 + GFF4683 0.31 +0.1 42,420 + GFF4683 0.31 -0.2 42,420 + GFF4683 0.31 -0.3 42,421 - GFF4683 0.31 +0.9 42,421 - GFF4683 0.31 +0.9
Or see this region's nucleotide sequence