Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF452 and GFF453 are separated by 97 nucleotides GFF453 and GFF454 overlap by 4 nucleotides GFF454 and GFF455 are separated by 303 nucleotides
GFF452 - Chloride channel protein, at 490,329 to 492,044
GFF452
GFF453 - hypothetical protein, at 492,142 to 492,366
GFF453
GFF454 - Z-ring-associated protein ZapA, at 492,363 to 492,707
GFF454
GFF455 - Uncharacterized UPF0721 integral membrane protein, at 493,011 to 493,838
GFF455
Position (kb)
492
493 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 491.448 kb on + strand, within GFF452 at 491.449 kb on - strand, within GFF452 at 491.538 kb on + strand, within GFF452 at 491.538 kb on + strand, within GFF452 at 491.539 kb on - strand, within GFF452 at 491.539 kb on - strand, within GFF452 at 491.539 kb on - strand, within GFF452 at 491.580 kb on + strand, within GFF452 at 491.581 kb on - strand, within GFF452 at 491.616 kb on + strand, within GFF452 at 491.616 kb on + strand, within GFF452 at 491.616 kb on + strand, within GFF452 at 491.616 kb on + strand, within GFF452 at 491.616 kb on + strand, within GFF452 at 491.616 kb on + strand, within GFF452 at 491.616 kb on + strand, within GFF452 at 491.617 kb on - strand, within GFF452 at 491.617 kb on - strand, within GFF452 at 491.617 kb on - strand, within GFF452 at 491.617 kb on - strand, within GFF452 at 491.617 kb on - strand, within GFF452 at 491.772 kb on + strand, within GFF452 at 491.772 kb on + strand, within GFF452 at 491.772 kb on + strand, within GFF452 at 491.772 kb on + strand, within GFF452 at 491.772 kb on + strand, within GFF452 at 491.773 kb on - strand, within GFF452 at 491.773 kb on - strand, within GFF452 at 491.773 kb on - strand, within GFF452 at 491.773 kb on - strand, within GFF452 at 491.773 kb on - strand, within GFF452 at 491.773 kb on - strand, within GFF452 at 491.773 kb on - strand, within GFF452 at 491.773 kb on - strand, within GFF452 at 491.773 kb on - strand, within GFF452 at 491.773 kb on - strand, within GFF452 at 491.778 kb on + strand, within GFF452 at 491.778 kb on + strand, within GFF452 at 491.778 kb on + strand, within GFF452 at 491.778 kb on + strand, within GFF452 at 491.778 kb on + strand, within GFF452 at 491.778 kb on + strand, within GFF452 at 491.778 kb on + strand, within GFF452 at 491.778 kb on + strand, within GFF452 at 491.778 kb on + strand, within GFF452 at 491.778 kb on + strand, within GFF452 at 491.779 kb on - strand, within GFF452 at 491.779 kb on - strand, within GFF452 at 491.779 kb on - strand, within GFF452 at 492.024 kb on + strand at 492.024 kb on + strand at 492.024 kb on + strand at 492.024 kb on + strand at 492.024 kb on + strand at 492.024 kb on + strand at 492.024 kb on + strand at 492.024 kb on + strand at 492.024 kb on + strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.043 kb on - strand at 492.043 kb on - strand at 492.043 kb on - strand at 492.043 kb on - strand at 492.088 kb on - strand at 492.128 kb on + strand at 492.128 kb on + strand at 492.128 kb on + strand at 492.128 kb on + strand at 492.129 kb on - strand at 492.129 kb on - strand at 492.129 kb on - strand at 492.379 kb on + strand at 492.379 kb on + strand at 492.379 kb on + strand at 492.380 kb on - strand at 492.380 kb on - strand at 492.399 kb on + strand, within GFF454 at 492.399 kb on + strand, within GFF454 at 492.399 kb on + strand, within GFF454 at 492.399 kb on + strand, within GFF454 at 492.400 kb on - strand, within GFF454 at 492.400 kb on - strand, within GFF454 at 492.400 kb on - strand, within GFF454 at 492.400 kb on - strand, within GFF454 at 492.592 kb on + strand, within GFF454 at 492.592 kb on + strand, within GFF454 at 492.592 kb on + strand, within GFF454 at 492.592 kb on + strand, within GFF454 at 492.592 kb on + strand, within GFF454 at 492.593 kb on - strand, within GFF454 at 492.626 kb on + strand, within GFF454 at 492.626 kb on + strand, within GFF454 at 492.626 kb on + strand, within GFF454 at 492.627 kb on - strand, within GFF454 at 492.738 kb on + strand at 492.774 kb on + strand at 492.774 kb on + strand at 492.775 kb on - strand at 492.775 kb on - strand at 492.775 kb on - strand at 492.812 kb on + strand at 492.812 kb on + strand at 492.812 kb on + strand at 492.812 kb on + strand at 492.813 kb on - strand at 492.813 kb on - strand at 492.813 kb on - strand at 492.813 kb on - strand at 492.813 kb on - strand at 492.813 kb on - strand at 492.813 kb on - strand at 492.820 kb on + strand at 492.821 kb on - strand at 492.911 kb on + strand at 493.002 kb on + strand at 493.003 kb on - strand at 493.620 kb on + strand, within GFF455 at 493.620 kb on + strand, within GFF455 at 493.620 kb on + strand, within GFF455 at 493.620 kb on + strand, within GFF455 at 493.621 kb on - strand, within GFF455 at 493.621 kb on - strand, within GFF455 at 493.621 kb on - strand, within GFF455 at 493.671 kb on + strand, within GFF455 at 493.671 kb on + strand, within GFF455 at 493.671 kb on + strand, within GFF455 at 493.672 kb on - strand, within GFF455 at 493.672 kb on - strand, within GFF455 at 493.672 kb on - strand, within GFF455
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 491,448 + GFF452 0.65 -0.7 491,449 - GFF452 0.65 -0.1 491,538 + GFF452 0.70 -1.0 491,538 + GFF452 0.70 -0.2 491,539 - GFF452 0.71 +0.1 491,539 - GFF452 0.71 -0.9 491,539 - GFF452 0.71 -1.6 491,580 + GFF452 0.73 -0.0 491,581 - GFF452 0.73 -0.8 491,616 + GFF452 0.75 +0.4 491,616 + GFF452 0.75 -0.1 491,616 + GFF452 0.75 -0.0 491,616 + GFF452 0.75 +0.8 491,616 + GFF452 0.75 +0.8 491,616 + GFF452 0.75 -0.4 491,616 + GFF452 0.75 -1.0 491,617 - GFF452 0.75 -0.1 491,617 - GFF452 0.75 -0.2 491,617 - GFF452 0.75 -0.7 491,617 - GFF452 0.75 -0.5 491,617 - GFF452 0.75 -0.5 491,772 + GFF452 0.84 -1.1 491,772 + GFF452 0.84 +0.1 491,772 + GFF452 0.84 +0.8 491,772 + GFF452 0.84 +0.2 491,772 + GFF452 0.84 -2.6 491,773 - GFF452 0.84 -0.0 491,773 - GFF452 0.84 -1.3 491,773 - GFF452 0.84 -0.7 491,773 - GFF452 0.84 -0.4 491,773 - GFF452 0.84 -1.2 491,773 - GFF452 0.84 -1.0 491,773 - GFF452 0.84 +1.2 491,773 - GFF452 0.84 -1.3 491,773 - GFF452 0.84 +0.0 491,773 - GFF452 0.84 +1.4 491,778 + GFF452 0.84 -0.5 491,778 + GFF452 0.84 +0.4 491,778 + GFF452 0.84 +0.2 491,778 + GFF452 0.84 +2.8 491,778 + GFF452 0.84 -1.2 491,778 + GFF452 0.84 -0.8 491,778 + GFF452 0.84 -0.9 491,778 + GFF452 0.84 +0.3 491,778 + GFF452 0.84 +0.7 491,778 + GFF452 0.84 +0.2 491,779 - GFF452 0.84 -0.2 491,779 - GFF452 0.84 -0.1 491,779 - GFF452 0.84 -0.7 492,024 + -0.2 492,024 + +0.2 492,024 + -0.9 492,024 + -0.6 492,024 + +0.1 492,024 + +0.1 492,024 + +0.6 492,024 + +1.0 492,024 + +0.5 492,025 - -0.7 492,025 - -0.8 492,025 - -0.1 492,025 - -0.4 492,025 - +0.8 492,025 - -1.1 492,025 - +0.2 492,025 - +0.4 492,025 - +0.0 492,043 - +0.1 492,043 - +0.2 492,043 - -0.2 492,043 - -0.8 492,088 - +0.3 492,128 + +0.4 492,128 + -0.4 492,128 + -0.7 492,128 + +0.8 492,129 - -1.0 492,129 - -0.1 492,129 - -0.0 492,379 + -0.6 492,379 + -1.0 492,379 + -0.9 492,380 - +0.3 492,380 - -0.7 492,399 + GFF454 0.10 +0.6 492,399 + GFF454 0.10 -0.7 492,399 + GFF454 0.10 -0.9 492,399 + GFF454 0.10 -0.1 492,400 - GFF454 0.11 +1.1 492,400 - GFF454 0.11 -1.3 492,400 - GFF454 0.11 -1.2 492,400 - GFF454 0.11 -1.2 492,592 + GFF454 0.66 +0.3 492,592 + GFF454 0.66 +0.1 492,592 + GFF454 0.66 +1.0 492,592 + GFF454 0.66 -0.0 492,592 + GFF454 0.66 +0.2 492,593 - GFF454 0.67 -0.3 492,626 + GFF454 0.76 -1.8 492,626 + GFF454 0.76 -1.2 492,626 + GFF454 0.76 -0.9 492,627 - GFF454 0.77 -1.5 492,738 + -0.6 492,774 + -0.6 492,774 + -0.9 492,775 - -0.9 492,775 - -0.8 492,775 - -0.2 492,812 + -0.6 492,812 + -0.0 492,812 + -0.4 492,812 + -1.7 492,813 - -0.3 492,813 - -0.2 492,813 - -1.6 492,813 - -0.3 492,813 - +0.3 492,813 - -0.8 492,813 - -1.2 492,820 + +0.5 492,821 - -0.7 492,911 + +0.5 493,002 + -0.0 493,003 - +0.2 493,620 + GFF455 0.74 +0.3 493,620 + GFF455 0.74 -0.8 493,620 + GFF455 0.74 -1.2 493,620 + GFF455 0.74 -1.8 493,621 - GFF455 0.74 +0.4 493,621 - GFF455 0.74 -0.9 493,621 - GFF455 0.74 -0.2 493,671 + GFF455 0.80 -0.4 493,671 + GFF455 0.80 -0.6 493,671 + GFF455 0.80 +1.0 493,672 - GFF455 0.80 -0.9 493,672 - GFF455 0.80 +0.4 493,672 - GFF455 0.80 -1.0
Or see this region's nucleotide sequence