Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4534 and GFF4535 overlap by 4 nucleotides GFF4535 and GFF4536 are separated by 18 nucleotides GFF4536 and GFF4537 overlap by 4 nucleotides
GFF4534 - Tetrapartite efflux system, outer membrane factor lipoprotein FusA-like, at 172,449 to 173,927
GFF4534
GFF4535 - Tetrapartite efflux system, membrane fusion component FusE-like, at 173,924 to 174,862
GFF4535
GFF4536 - Tetrapartite efflux system component, FusD-like, at 174,881 to 175,087
GFF4536
GFF4537 - Tetrapartite efflux system, inner membrane component FusBC-like, at 175,084 to 177,216
GFF4537
Position (kb)
173
174
175 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 172.966 kb on + strand, within GFF4534 at 172.966 kb on + strand, within GFF4534 at 173.167 kb on + strand, within GFF4534 at 173.168 kb on - strand, within GFF4534 at 173.369 kb on - strand, within GFF4534 at 173.369 kb on - strand, within GFF4534 at 173.491 kb on + strand, within GFF4534 at 173.491 kb on + strand, within GFF4534 at 173.492 kb on - strand, within GFF4534 at 173.515 kb on + strand, within GFF4534 at 173.515 kb on + strand, within GFF4534 at 173.515 kb on + strand, within GFF4534 at 173.515 kb on + strand, within GFF4534 at 173.515 kb on + strand, within GFF4534 at 173.515 kb on + strand, within GFF4534 at 173.515 kb on + strand, within GFF4534 at 173.516 kb on - strand, within GFF4534 at 173.516 kb on - strand, within GFF4534 at 173.516 kb on - strand, within GFF4534 at 173.516 kb on - strand, within GFF4534 at 173.516 kb on - strand, within GFF4534 at 173.516 kb on - strand, within GFF4534 at 173.539 kb on + strand, within GFF4534 at 173.539 kb on + strand, within GFF4534 at 173.540 kb on - strand, within GFF4534 at 173.540 kb on - strand, within GFF4534 at 173.540 kb on - strand, within GFF4534 at 173.540 kb on - strand, within GFF4534 at 173.578 kb on + strand, within GFF4534 at 173.579 kb on - strand, within GFF4534 at 173.611 kb on + strand, within GFF4534 at 173.611 kb on + strand, within GFF4534 at 173.611 kb on + strand, within GFF4534 at 173.612 kb on - strand, within GFF4534 at 173.612 kb on - strand, within GFF4534 at 173.612 kb on - strand, within GFF4534 at 173.734 kb on + strand, within GFF4534 at 173.839 kb on + strand at 174.255 kb on + strand, within GFF4535 at 174.256 kb on - strand, within GFF4535 at 174.256 kb on - strand, within GFF4535 at 174.256 kb on - strand, within GFF4535 at 174.256 kb on - strand, within GFF4535 at 174.267 kb on + strand, within GFF4535 at 174.267 kb on + strand, within GFF4535 at 174.267 kb on + strand, within GFF4535 at 174.267 kb on + strand, within GFF4535 at 174.267 kb on + strand, within GFF4535 at 174.268 kb on - strand, within GFF4535 at 174.318 kb on + strand, within GFF4535 at 174.318 kb on + strand, within GFF4535 at 174.318 kb on + strand, within GFF4535 at 174.318 kb on + strand, within GFF4535 at 174.318 kb on + strand, within GFF4535 at 174.318 kb on + strand, within GFF4535 at 174.318 kb on + strand, within GFF4535 at 174.318 kb on + strand, within GFF4535 at 174.319 kb on - strand, within GFF4535 at 174.319 kb on - strand, within GFF4535 at 174.592 kb on - strand, within GFF4535 at 174.744 kb on + strand, within GFF4535 at 174.744 kb on + strand, within GFF4535 at 174.744 kb on + strand, within GFF4535 at 174.745 kb on - strand, within GFF4535 at 174.745 kb on - strand, within GFF4535 at 174.745 kb on - strand, within GFF4535 at 174.875 kb on - strand at 174.878 kb on + strand at 174.879 kb on - strand at 174.879 kb on - strand at 174.879 kb on - strand at 174.879 kb on - strand at 174.882 kb on + strand at 174.882 kb on + strand at 174.882 kb on + strand at 174.882 kb on + strand at 174.882 kb on + strand at 174.883 kb on - strand at 174.891 kb on + strand at 174.891 kb on + strand at 174.891 kb on + strand at 174.891 kb on + strand at 174.892 kb on - strand at 174.892 kb on - strand at 174.892 kb on - strand at 174.915 kb on + strand, within GFF4536 at 174.916 kb on - strand, within GFF4536 at 174.930 kb on + strand, within GFF4536 at 174.930 kb on + strand, within GFF4536 at 174.930 kb on + strand, within GFF4536 at 174.931 kb on - strand, within GFF4536 at 174.931 kb on - strand, within GFF4536 at 174.931 kb on - strand, within GFF4536 at 174.972 kb on + strand, within GFF4536 at 174.972 kb on + strand, within GFF4536 at 175.056 kb on + strand, within GFF4536 at 175.056 kb on + strand, within GFF4536 at 175.056 kb on + strand, within GFF4536 at 175.056 kb on + strand, within GFF4536 at 175.056 kb on + strand, within GFF4536 at 175.056 kb on + strand, within GFF4536 at 175.056 kb on + strand, within GFF4536 at 175.056 kb on + strand, within GFF4536 at 175.057 kb on - strand, within GFF4536 at 175.057 kb on - strand, within GFF4536 at 175.057 kb on - strand, within GFF4536 at 175.057 kb on - strand, within GFF4536 at 175.057 kb on - strand, within GFF4536 at 175.057 kb on - strand, within GFF4536 at 175.496 kb on + strand, within GFF4537 at 175.496 kb on + strand, within GFF4537 at 175.496 kb on + strand, within GFF4537 at 175.496 kb on + strand, within GFF4537 at 175.496 kb on + strand, within GFF4537 at 175.497 kb on - strand, within GFF4537 at 175.497 kb on - strand, within GFF4537 at 175.497 kb on - strand, within GFF4537 at 175.497 kb on - strand, within GFF4537 at 175.497 kb on - strand, within GFF4537 at 175.497 kb on - strand, within GFF4537 at 175.514 kb on + strand, within GFF4537 at 175.515 kb on - strand, within GFF4537 at 175.515 kb on - strand, within GFF4537 at 175.772 kb on + strand, within GFF4537 at 175.772 kb on + strand, within GFF4537 at 175.772 kb on + strand, within GFF4537 at 175.772 kb on + strand, within GFF4537 at 175.772 kb on + strand, within GFF4537 at 175.773 kb on - strand, within GFF4537 at 175.773 kb on - strand, within GFF4537 at 175.773 kb on - strand, within GFF4537 at 175.773 kb on - strand, within GFF4537 at 175.773 kb on - strand, within GFF4537 at 175.773 kb on - strand, within GFF4537 at 175.856 kb on + strand, within GFF4537 at 175.856 kb on + strand, within GFF4537 at 175.856 kb on + strand, within GFF4537 at 175.856 kb on + strand, within GFF4537 at 175.856 kb on + strand, within GFF4537 at 175.856 kb on + strand, within GFF4537 at 175.856 kb on + strand, within GFF4537 at 175.856 kb on + strand, within GFF4537 at 175.857 kb on - strand, within GFF4537 at 175.857 kb on - strand, within GFF4537 at 175.857 kb on - strand, within GFF4537 at 175.857 kb on - strand, within GFF4537 at 175.857 kb on - strand, within GFF4537 at 175.857 kb on - strand, within GFF4537 at 175.857 kb on - strand, within GFF4537
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 172,966 + GFF4534 0.35 -1.5 172,966 + GFF4534 0.35 -1.5 173,167 + GFF4534 0.49 +0.3 173,168 - GFF4534 0.49 +1.3 173,369 - GFF4534 0.62 -1.4 173,369 - GFF4534 0.62 +0.1 173,491 + GFF4534 0.70 -0.3 173,491 + GFF4534 0.70 +0.2 173,492 - GFF4534 0.71 +0.2 173,515 + GFF4534 0.72 -0.4 173,515 + GFF4534 0.72 +0.2 173,515 + GFF4534 0.72 +1.5 173,515 + GFF4534 0.72 +1.4 173,515 + GFF4534 0.72 -0.4 173,515 + GFF4534 0.72 -0.1 173,515 + GFF4534 0.72 -0.0 173,516 - GFF4534 0.72 +0.5 173,516 - GFF4534 0.72 -0.9 173,516 - GFF4534 0.72 +1.8 173,516 - GFF4534 0.72 +0.0 173,516 - GFF4534 0.72 -0.4 173,516 - GFF4534 0.72 -0.6 173,539 + GFF4534 0.74 -0.3 173,539 + GFF4534 0.74 -0.0 173,540 - GFF4534 0.74 +0.2 173,540 - GFF4534 0.74 +0.6 173,540 - GFF4534 0.74 +0.4 173,540 - GFF4534 0.74 -0.8 173,578 + GFF4534 0.76 +0.2 173,579 - GFF4534 0.76 -0.6 173,611 + GFF4534 0.79 +0.2 173,611 + GFF4534 0.79 +1.7 173,611 + GFF4534 0.79 -0.1 173,612 - GFF4534 0.79 -0.3 173,612 - GFF4534 0.79 -1.7 173,612 - GFF4534 0.79 +0.6 173,734 + GFF4534 0.87 -0.5 173,839 + -1.6 174,255 + GFF4535 0.35 -1.4 174,256 - GFF4535 0.35 +0.4 174,256 - GFF4535 0.35 +0.5 174,256 - GFF4535 0.35 +0.4 174,256 - GFF4535 0.35 -0.6 174,267 + GFF4535 0.37 +1.8 174,267 + GFF4535 0.37 +0.9 174,267 + GFF4535 0.37 -0.9 174,267 + GFF4535 0.37 +0.4 174,267 + GFF4535 0.37 -1.0 174,268 - GFF4535 0.37 -0.5 174,318 + GFF4535 0.42 -1.7 174,318 + GFF4535 0.42 -0.1 174,318 + GFF4535 0.42 +0.4 174,318 + GFF4535 0.42 -0.4 174,318 + GFF4535 0.42 -0.6 174,318 + GFF4535 0.42 +1.2 174,318 + GFF4535 0.42 +0.4 174,318 + GFF4535 0.42 -1.1 174,319 - GFF4535 0.42 -1.2 174,319 - GFF4535 0.42 -0.0 174,592 - GFF4535 0.71 -0.3 174,744 + GFF4535 0.87 +2.2 174,744 + GFF4535 0.87 -0.7 174,744 + GFF4535 0.87 +2.2 174,745 - GFF4535 0.87 -0.7 174,745 - GFF4535 0.87 -0.8 174,745 - GFF4535 0.87 -0.4 174,875 - -0.4 174,878 + +0.6 174,879 - +0.6 174,879 - -3.1 174,879 - -0.5 174,879 - -0.3 174,882 + +0.9 174,882 + -0.2 174,882 + -1.2 174,882 + +0.3 174,882 + +0.3 174,883 - -0.4 174,891 + -0.1 174,891 + +1.7 174,891 + -0.1 174,891 + -0.4 174,892 - -0.2 174,892 - -0.3 174,892 - -0.3 174,915 + GFF4536 0.16 -0.4 174,916 - GFF4536 0.17 -0.3 174,930 + GFF4536 0.24 +0.6 174,930 + GFF4536 0.24 +0.2 174,930 + GFF4536 0.24 -2.7 174,931 - GFF4536 0.24 +0.3 174,931 - GFF4536 0.24 +0.2 174,931 - GFF4536 0.24 -0.9 174,972 + GFF4536 0.44 -0.6 174,972 + GFF4536 0.44 -0.8 175,056 + GFF4536 0.85 -0.3 175,056 + GFF4536 0.85 -1.3 175,056 + GFF4536 0.85 -1.4 175,056 + GFF4536 0.85 -0.4 175,056 + GFF4536 0.85 -0.0 175,056 + GFF4536 0.85 +0.1 175,056 + GFF4536 0.85 +1.0 175,056 + GFF4536 0.85 +0.4 175,057 - GFF4536 0.85 +0.2 175,057 - GFF4536 0.85 +1.1 175,057 - GFF4536 0.85 +0.6 175,057 - GFF4536 0.85 -0.1 175,057 - GFF4536 0.85 -0.0 175,057 - GFF4536 0.85 -0.0 175,496 + GFF4537 0.19 -0.9 175,496 + GFF4537 0.19 -0.1 175,496 + GFF4537 0.19 -0.7 175,496 + GFF4537 0.19 +0.2 175,496 + GFF4537 0.19 +0.2 175,497 - GFF4537 0.19 -0.1 175,497 - GFF4537 0.19 -0.3 175,497 - GFF4537 0.19 -0.8 175,497 - GFF4537 0.19 +0.4 175,497 - GFF4537 0.19 -0.1 175,497 - GFF4537 0.19 -1.5 175,514 + GFF4537 0.20 -0.4 175,515 - GFF4537 0.20 +0.8 175,515 - GFF4537 0.20 +1.0 175,772 + GFF4537 0.32 -0.2 175,772 + GFF4537 0.32 -0.7 175,772 + GFF4537 0.32 -2.1 175,772 + GFF4537 0.32 +1.8 175,772 + GFF4537 0.32 -0.8 175,773 - GFF4537 0.32 +0.6 175,773 - GFF4537 0.32 -2.0 175,773 - GFF4537 0.32 +0.2 175,773 - GFF4537 0.32 +0.3 175,773 - GFF4537 0.32 -2.3 175,773 - GFF4537 0.32 -0.1 175,856 + GFF4537 0.36 +1.9 175,856 + GFF4537 0.36 +0.1 175,856 + GFF4537 0.36 +0.2 175,856 + GFF4537 0.36 +1.2 175,856 + GFF4537 0.36 -0.1 175,856 + GFF4537 0.36 +0.2 175,856 + GFF4537 0.36 -0.4 175,856 + GFF4537 0.36 +1.0 175,857 - GFF4537 0.36 -0.6 175,857 - GFF4537 0.36 +0.4 175,857 - GFF4537 0.36 -0.1 175,857 - GFF4537 0.36 -0.9 175,857 - GFF4537 0.36 +1.4 175,857 - GFF4537 0.36 +1.2 175,857 - GFF4537 0.36 +0.3
Or see this region's nucleotide sequence