Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4313 and GFF4314 are separated by 18 nucleotides GFF4314 and GFF4315 overlap by 4 nucleotides GFF4315 and GFF4316 overlap by 4 nucleotides
GFF4313 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 233,836 to 234,840
GFF4313
GFF4314 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 234,859 to 235,662
GFF4314
GFF4315 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 235,659 to 236,528
GFF4315
GFF4316 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 236,525 to 238,327
GFF4316
Position (kb) 234
235
236 Strain fitness (log2 ratio) -2
-1
0
1
2 at 234.515 kb on + strand, within GFF4313 at 234.516 kb on - strand, within GFF4313 at 234.630 kb on - strand, within GFF4313 at 234.630 kb on - strand, within GFF4313 at 234.630 kb on - strand, within GFF4313 at 234.630 kb on - strand, within GFF4313 at 234.630 kb on - strand, within GFF4313 at 234.824 kb on + strand at 234.824 kb on + strand at 234.825 kb on - strand at 234.915 kb on - strand at 235.128 kb on - strand, within GFF4314 at 235.157 kb on + strand, within GFF4314 at 235.157 kb on + strand, within GFF4314 at 235.181 kb on + strand, within GFF4314 at 235.181 kb on + strand, within GFF4314 at 235.182 kb on - strand, within GFF4314 at 235.182 kb on - strand, within GFF4314 at 235.601 kb on + strand at 235.754 kb on - strand, within GFF4315 at 235.831 kb on + strand, within GFF4315 at 236.417 kb on - strand, within GFF4315 at 236.489 kb on + strand at 236.490 kb on - strand at 236.490 kb on - strand at 236.559 kb on + strand at 236.560 kb on - strand at 236.560 kb on - strand at 236.560 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 234,515 + GFF4313 0.68 +0.2 234,516 - GFF4313 0.68 -0.7 234,630 - GFF4313 0.79 -0.7 234,630 - GFF4313 0.79 +0.1 234,630 - GFF4313 0.79 +0.9 234,630 - GFF4313 0.79 +0.7 234,630 - GFF4313 0.79 -0.4 234,824 + -0.4 234,824 + +1.6 234,825 - +2.2 234,915 - -0.4 235,128 - GFF4314 0.33 +0.3 235,157 + GFF4314 0.37 +0.4 235,157 + GFF4314 0.37 -0.5 235,181 + GFF4314 0.40 -1.0 235,181 + GFF4314 0.40 -0.3 235,182 - GFF4314 0.40 -1.5 235,182 - GFF4314 0.40 -0.8 235,601 + -0.4 235,754 - GFF4315 0.11 -1.9 235,831 + GFF4315 0.20 -0.5 236,417 - GFF4315 0.87 +0.2 236,489 + -1.2 236,490 - -0.3 236,490 - -1.5 236,559 + +2.2 236,560 - -0.7 236,560 - -0.9 236,560 - -0.0
Or see this region's nucleotide sequence