Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF420 and GFF422 are separated by 10 nucleotides GFF422 and GFF421 overlap by 9 nucleotides GFF421 and GFF423 are separated by 31 nucleotides GFF423 and GFF424 overlap by 4 nucleotides
GFF420 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 454,739 to 456,301
GFF420
GFF422 - no description, at 456,312 to 456,452
GFF422
GFF421 - hypothetical protein, at 456,444 to 459,227
GFF421
GFF423 - Alpha-ribazole-5'-phosphate phosphatase (EC 3.1.3.73), at 459,259 to 459,822
GFF423
GFF424 - Cobalamin synthase (EC 2.7.8.26), at 459,819 to 460,613
GFF424
Position (kb) 456
457
458
459
460 Strain fitness (log2 ratio) -2
-1
0
1
2 at 455.730 kb on + strand, within GFF420 at 455.910 kb on + strand, within GFF420 at 455.910 kb on + strand, within GFF420 at 455.910 kb on + strand, within GFF420 at 455.910 kb on + strand, within GFF420 at 455.910 kb on + strand, within GFF420 at 455.910 kb on + strand, within GFF420 at 455.911 kb on - strand, within GFF420 at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.303 kb on + strand at 456.304 kb on - strand at 456.304 kb on - strand at 456.304 kb on - strand at 456.304 kb on - strand at 456.304 kb on - strand at 456.304 kb on - strand at 456.304 kb on - strand at 456.304 kb on - strand at 456.304 kb on - strand at 456.304 kb on - strand at 456.304 kb on - strand at 456.304 kb on - strand at 456.304 kb on - strand at 456.400 kb on + strand, within GFF422 at 456.400 kb on + strand, within GFF422 at 456.400 kb on + strand, within GFF422 at 456.400 kb on + strand, within GFF422 at 456.400 kb on + strand, within GFF422 at 456.400 kb on + strand, within GFF422 at 456.401 kb on - strand, within GFF422 at 456.401 kb on - strand, within GFF422 at 456.401 kb on - strand, within GFF422 at 456.401 kb on - strand, within GFF422 at 456.401 kb on - strand, within GFF422 at 456.401 kb on - strand, within GFF422 at 456.401 kb on - strand, within GFF422 at 456.401 kb on - strand, within GFF422 at 456.401 kb on - strand, within GFF422 at 456.401 kb on - strand, within GFF422 at 456.401 kb on - strand, within GFF422 at 456.771 kb on + strand, within GFF421 at 456.772 kb on - strand, within GFF421 at 456.772 kb on - strand, within GFF421 at 456.772 kb on - strand, within GFF421 at 456.906 kb on + strand, within GFF421 at 457.044 kb on + strand, within GFF421 at 457.044 kb on + strand, within GFF421 at 457.045 kb on - strand, within GFF421 at 457.045 kb on - strand, within GFF421 at 457.045 kb on - strand, within GFF421 at 457.045 kb on - strand, within GFF421 at 457.218 kb on + strand, within GFF421 at 457.218 kb on + strand, within GFF421 at 457.218 kb on + strand, within GFF421 at 457.218 kb on + strand, within GFF421 at 457.219 kb on - strand, within GFF421 at 457.219 kb on - strand, within GFF421 at 457.219 kb on - strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.470 kb on + strand, within GFF421 at 457.471 kb on - strand, within GFF421 at 457.471 kb on - strand, within GFF421 at 457.471 kb on - strand, within GFF421 at 457.471 kb on - strand, within GFF421 at 457.471 kb on - strand, within GFF421 at 457.471 kb on - strand, within GFF421 at 457.471 kb on - strand, within GFF421 at 457.783 kb on - strand, within GFF421 at 457.783 kb on - strand, within GFF421 at 458.004 kb on + strand, within GFF421 at 458.004 kb on + strand, within GFF421 at 458.004 kb on + strand, within GFF421 at 458.005 kb on - strand, within GFF421 at 458.005 kb on - strand, within GFF421 at 458.005 kb on - strand, within GFF421 at 458.005 kb on - strand, within GFF421 at 458.005 kb on - strand, within GFF421 at 458.005 kb on - strand, within GFF421 at 458.136 kb on + strand, within GFF421 at 458.136 kb on + strand, within GFF421 at 458.136 kb on + strand, within GFF421 at 458.136 kb on + strand, within GFF421 at 458.136 kb on + strand, within GFF421 at 458.136 kb on + strand, within GFF421 at 458.137 kb on - strand, within GFF421 at 458.137 kb on - strand, within GFF421 at 458.137 kb on - strand, within GFF421 at 458.137 kb on - strand, within GFF421 at 458.137 kb on - strand, within GFF421 at 458.137 kb on - strand, within GFF421 at 458.137 kb on - strand, within GFF421 at 458.175 kb on + strand, within GFF421 at 458.175 kb on + strand, within GFF421 at 458.176 kb on - strand, within GFF421 at 458.176 kb on - strand, within GFF421 at 458.176 kb on - strand, within GFF421 at 458.176 kb on - strand, within GFF421 at 458.176 kb on - strand, within GFF421 at 458.176 kb on - strand, within GFF421 at 458.265 kb on + strand, within GFF421 at 458.265 kb on + strand, within GFF421 at 458.265 kb on + strand, within GFF421 at 458.265 kb on + strand, within GFF421 at 458.265 kb on + strand, within GFF421 at 458.265 kb on + strand, within GFF421 at 458.266 kb on - strand, within GFF421 at 458.266 kb on - strand, within GFF421 at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.225 kb on + strand at 459.225 kb on + strand at 459.225 kb on + strand at 459.225 kb on + strand at 459.225 kb on + strand at 459.225 kb on + strand at 459.225 kb on + strand at 459.225 kb on + strand at 459.226 kb on - strand at 459.226 kb on - strand at 459.226 kb on - strand at 459.226 kb on - strand at 459.226 kb on - strand at 459.226 kb on - strand at 459.226 kb on - strand at 459.576 kb on - strand, within GFF423 at 459.764 kb on + strand, within GFF423 at 459.765 kb on - strand, within GFF423 at 459.765 kb on - strand, within GFF423 at 459.844 kb on + strand at 459.844 kb on + strand at 459.844 kb on + strand at 459.845 kb on - strand at 459.845 kb on - strand at 459.845 kb on - strand at 460.006 kb on - strand, within GFF424 at 460.006 kb on - strand, within GFF424 at 460.006 kb on - strand, within GFF424 at 460.102 kb on + strand, within GFF424 at 460.102 kb on + strand, within GFF424 at 460.102 kb on + strand, within GFF424 at 460.103 kb on - strand, within GFF424 at 460.103 kb on - strand, within GFF424 at 460.103 kb on - strand, within GFF424 at 460.103 kb on - strand, within GFF424 at 460.103 kb on - strand, within GFF424 at 460.103 kb on - strand, within GFF424 at 460.103 kb on - strand, within GFF424
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 455,730 + GFF420 0.63 -0.3 455,910 + GFF420 0.75 -0.1 455,910 + GFF420 0.75 +0.2 455,910 + GFF420 0.75 -1.6 455,910 + GFF420 0.75 -0.2 455,910 + GFF420 0.75 +1.4 455,910 + GFF420 0.75 +1.6 455,911 - GFF420 0.75 -0.4 456,303 + +0.2 456,303 + -0.8 456,303 + +0.4 456,303 + +0.3 456,303 + -0.4 456,303 + -0.2 456,303 + +0.8 456,303 + -0.0 456,303 + -0.4 456,303 + -1.0 456,303 + +1.2 456,303 + -1.9 456,303 + -0.2 456,303 + +0.3 456,303 + -1.0 456,303 + -2.1 456,303 + +0.2 456,303 + -1.4 456,303 + -0.0 456,303 + +0.6 456,304 - +0.4 456,304 - -1.4 456,304 - -0.3 456,304 - -0.6 456,304 - -1.0 456,304 - +0.2 456,304 - -0.4 456,304 - -0.2 456,304 - +0.9 456,304 - -0.6 456,304 - +0.3 456,304 - +0.9 456,304 - -0.0 456,400 + GFF422 0.62 +0.3 456,400 + GFF422 0.62 -1.0 456,400 + GFF422 0.62 -0.2 456,400 + GFF422 0.62 -0.6 456,400 + GFF422 0.62 -0.2 456,400 + GFF422 0.62 -0.5 456,401 - GFF422 0.63 -0.1 456,401 - GFF422 0.63 +0.3 456,401 - GFF422 0.63 +0.9 456,401 - GFF422 0.63 +1.1 456,401 - GFF422 0.63 -0.2 456,401 - GFF422 0.63 -0.0 456,401 - GFF422 0.63 -1.0 456,401 - GFF422 0.63 +1.2 456,401 - GFF422 0.63 +1.8 456,401 - GFF422 0.63 -0.0 456,401 - GFF422 0.63 -0.9 456,771 + GFF421 0.12 -0.4 456,772 - GFF421 0.12 -0.8 456,772 - GFF421 0.12 +1.8 456,772 - GFF421 0.12 -1.0 456,906 + GFF421 0.17 -1.2 457,044 + GFF421 0.22 -1.9 457,044 + GFF421 0.22 +0.1 457,045 - GFF421 0.22 -0.5 457,045 - GFF421 0.22 -1.0 457,045 - GFF421 0.22 -0.8 457,045 - GFF421 0.22 -0.2 457,218 + GFF421 0.28 -1.8 457,218 + GFF421 0.28 -0.6 457,218 + GFF421 0.28 -1.4 457,218 + GFF421 0.28 +0.2 457,219 - GFF421 0.28 +0.4 457,219 - GFF421 0.28 +0.8 457,219 - GFF421 0.28 -1.0 457,470 + GFF421 0.37 -1.7 457,470 + GFF421 0.37 -0.3 457,470 + GFF421 0.37 -0.4 457,470 + GFF421 0.37 +0.4 457,470 + GFF421 0.37 -0.6 457,470 + GFF421 0.37 -0.6 457,470 + GFF421 0.37 +0.2 457,470 + GFF421 0.37 -1.6 457,470 + GFF421 0.37 -0.1 457,470 + GFF421 0.37 -1.8 457,470 + GFF421 0.37 +0.0 457,470 + GFF421 0.37 +0.2 457,470 + GFF421 0.37 -2.2 457,470 + GFF421 0.37 +0.2 457,470 + GFF421 0.37 -1.5 457,470 + GFF421 0.37 -1.6 457,470 + GFF421 0.37 -0.5 457,471 - GFF421 0.37 -0.1 457,471 - GFF421 0.37 +0.1 457,471 - GFF421 0.37 +0.4 457,471 - GFF421 0.37 -0.7 457,471 - GFF421 0.37 +0.3 457,471 - GFF421 0.37 +0.3 457,471 - GFF421 0.37 +2.2 457,783 - GFF421 0.48 -0.2 457,783 - GFF421 0.48 +0.5 458,004 + GFF421 0.56 -0.4 458,004 + GFF421 0.56 -1.7 458,004 + GFF421 0.56 -0.7 458,005 - GFF421 0.56 +0.2 458,005 - GFF421 0.56 +0.4 458,005 - GFF421 0.56 -1.0 458,005 - GFF421 0.56 -1.3 458,005 - GFF421 0.56 +0.9 458,005 - GFF421 0.56 -0.8 458,136 + GFF421 0.61 -0.5 458,136 + GFF421 0.61 -0.1 458,136 + GFF421 0.61 -0.7 458,136 + GFF421 0.61 -1.6 458,136 + GFF421 0.61 +0.8 458,136 + GFF421 0.61 -0.3 458,137 - GFF421 0.61 -0.7 458,137 - GFF421 0.61 +0.0 458,137 - GFF421 0.61 -0.9 458,137 - GFF421 0.61 -0.5 458,137 - GFF421 0.61 -1.0 458,137 - GFF421 0.61 -1.8 458,137 - GFF421 0.61 +0.4 458,175 + GFF421 0.62 -0.4 458,175 + GFF421 0.62 +1.0 458,176 - GFF421 0.62 -0.7 458,176 - GFF421 0.62 -0.2 458,176 - GFF421 0.62 -0.7 458,176 - GFF421 0.62 -1.0 458,176 - GFF421 0.62 +1.8 458,176 - GFF421 0.62 +0.4 458,265 + GFF421 0.65 -0.3 458,265 + GFF421 0.65 -0.0 458,265 + GFF421 0.65 -0.2 458,265 + GFF421 0.65 +0.2 458,265 + GFF421 0.65 -0.2 458,265 + GFF421 0.65 -1.2 458,266 - GFF421 0.65 -0.3 458,266 - GFF421 0.65 -0.9 459,144 + -2.1 459,144 + +0.1 459,144 + -1.0 459,144 + -0.8 459,144 + -0.5 459,144 + -0.5 459,144 + -0.5 459,144 + -2.6 459,144 + -0.6 459,144 + +0.6 459,144 + -0.2 459,144 + -0.1 459,144 + -0.8 459,144 + +0.1 459,144 + -0.4 459,144 + -0.2 459,144 + -0.8 459,144 + +0.7 459,144 + +0.0 459,144 + +0.4 459,144 + +1.8 459,145 - +1.8 459,145 - -0.3 459,145 - -0.3 459,145 - +0.0 459,145 - +0.2 459,145 - -0.8 459,145 - -0.2 459,145 - -1.3 459,145 - -0.1 459,145 - +0.1 459,145 - -0.6 459,145 - +1.4 459,145 - +0.3 459,145 - -0.0 459,145 - -0.1 459,145 - -0.0 459,145 - -0.6 459,145 - -0.4 459,225 + +0.5 459,225 + +0.2 459,225 + -1.0 459,225 + -0.1 459,225 + -1.8 459,225 + -0.5 459,225 + +0.4 459,225 + -1.4 459,226 - +0.8 459,226 - -0.0 459,226 - -0.7 459,226 - +0.2 459,226 - -1.8 459,226 - +0.2 459,226 - +0.6 459,576 - GFF423 0.56 -0.8 459,764 + GFF423 0.90 +0.1 459,765 - GFF423 0.90 -0.2 459,765 - GFF423 0.90 -0.7 459,844 + -1.1 459,844 + -0.6 459,844 + -0.8 459,845 - -1.1 459,845 - +0.9 459,845 - -0.7 460,006 - GFF424 0.24 +0.1 460,006 - GFF424 0.24 -1.7 460,006 - GFF424 0.24 -0.0 460,102 + GFF424 0.36 +0.0 460,102 + GFF424 0.36 +0.5 460,102 + GFF424 0.36 +0.5 460,103 - GFF424 0.36 -0.4 460,103 - GFF424 0.36 +0.6 460,103 - GFF424 0.36 -1.7 460,103 - GFF424 0.36 -0.6 460,103 - GFF424 0.36 +1.2 460,103 - GFF424 0.36 +0.2 460,103 - GFF424 0.36 -0.6
Or see this region's nucleotide sequence