Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4165 and GFF4166 overlap by 11 nucleotides GFF4166 and GFF4167 overlap by 4 nucleotides GFF4167 and GFF4168 are separated by 1 nucleotides GFF4168 and GFF4169 overlap by 4 nucleotides
GFF4165 - Deoxyribose-phosphate aldolase (EC 4.1.2.4), at 70,247 to 71,041
GFF4165
GFF4166 - Cytidine deaminase (EC 3.5.4.5), at 71,031 to 71,501
GFF4166
GFF4167 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines) / ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 71,498 to 73,120
GFF4167
GFF4168 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 73,122 to 73,937
GFF4168
GFF4169 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 73,934 to 74,878
GFF4169
Position (kb) 71
72
73
74 Strain fitness (log2 ratio) -2
-1
0
1 at 71.419 kb on + strand, within GFF4166 at 71.566 kb on - strand at 72.314 kb on + strand, within GFF4167 at 72.314 kb on + strand, within GFF4167 at 72.366 kb on + strand, within GFF4167 at 72.366 kb on + strand, within GFF4167 at 72.367 kb on - strand, within GFF4167 at 72.367 kb on - strand, within GFF4167 at 72.367 kb on - strand, within GFF4167 at 73.453 kb on + strand, within GFF4168 at 73.454 kb on - strand, within GFF4168 at 73.454 kb on - strand, within GFF4168 at 73.454 kb on - strand, within GFF4168 at 73.454 kb on - strand, within GFF4168 at 73.968 kb on + strand at 74.001 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 71,419 + GFF4166 0.82 -0.1 71,566 - +0.7 72,314 + GFF4167 0.50 -1.5 72,314 + GFF4167 0.50 -0.1 72,366 + GFF4167 0.53 -2.4 72,366 + GFF4167 0.53 +0.3 72,367 - GFF4167 0.54 -0.4 72,367 - GFF4167 0.54 -0.4 72,367 - GFF4167 0.54 +0.7 73,453 + GFF4168 0.41 +0.6 73,454 - GFF4168 0.41 +0.8 73,454 - GFF4168 0.41 -0.0 73,454 - GFF4168 0.41 -0.4 73,454 - GFF4168 0.41 +0.3 73,968 + -0.3 74,001 + -0.4
Or see this region's nucleotide sequence