Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF40 and GFF41 are separated by 180 nucleotides GFF41 and GFF42 are separated by 69 nucleotides GFF42 and GFF43 are separated by 247 nucleotides
GFF40 - no description, at 42,447 to 43,592
GFF40
GFF41 - no description, at 43,773 to 44,096
GFF41
GFF42 - hypothetical protein, at 44,166 to 44,612
GFF42
GFF43 - hypothetical protein, at 44,860 to 46,923
GFF43
Position (kb) 43
44
45 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 42.784 kb on + strand, within GFF40 at 42.784 kb on + strand, within GFF40 at 42.970 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.040 kb on - strand, within GFF40 at 43.040 kb on - strand, within GFF40 at 43.165 kb on + strand, within GFF40 at 43.166 kb on - strand, within GFF40 at 43.343 kb on - strand, within GFF40 at 43.343 kb on - strand, within GFF40 at 43.534 kb on + strand at 43.534 kb on + strand at 43.534 kb on + strand at 43.534 kb on + strand at 43.534 kb on + strand at 43.534 kb on + strand at 43.534 kb on + strand at 43.535 kb on - strand at 43.535 kb on - strand at 43.535 kb on - strand at 43.537 kb on + strand at 43.583 kb on + strand at 43.584 kb on - strand at 43.615 kb on + strand at 43.616 kb on - strand at 43.628 kb on + strand at 43.628 kb on + strand at 43.628 kb on + strand at 43.628 kb on + strand at 43.628 kb on + strand at 43.628 kb on + strand at 43.628 kb on + strand at 43.628 kb on + strand at 43.628 kb on + strand at 43.628 kb on + strand at 43.628 kb on + strand at 43.628 kb on + strand at 43.628 kb on + strand at 43.629 kb on - strand at 43.629 kb on - strand at 43.629 kb on - strand at 43.629 kb on - strand at 43.629 kb on - strand at 43.629 kb on - strand at 43.629 kb on - strand at 43.857 kb on + strand, within GFF41 at 43.857 kb on + strand, within GFF41 at 43.857 kb on + strand, within GFF41 at 43.857 kb on + strand, within GFF41 at 43.858 kb on - strand, within GFF41 at 43.885 kb on + strand, within GFF41 at 43.897 kb on + strand, within GFF41 at 44.085 kb on + strand at 44.085 kb on + strand at 44.085 kb on + strand at 44.086 kb on - strand at 44.141 kb on + strand at 44.142 kb on - strand at 44.142 kb on - strand at 44.197 kb on + strand at 44.197 kb on + strand at 44.197 kb on + strand at 44.197 kb on + strand at 44.197 kb on + strand at 44.197 kb on + strand at 44.198 kb on - strand at 44.198 kb on - strand at 44.198 kb on - strand at 44.198 kb on - strand at 44.198 kb on - strand at 44.198 kb on - strand at 44.198 kb on - strand at 44.269 kb on - strand, within GFF42 at 44.508 kb on - strand, within GFF42 at 44.554 kb on + strand, within GFF42 at 44.554 kb on + strand, within GFF42 at 44.554 kb on + strand, within GFF42 at 44.554 kb on + strand, within GFF42 at 44.554 kb on + strand, within GFF42 at 44.555 kb on - strand, within GFF42 at 44.555 kb on - strand, within GFF42 at 44.555 kb on - strand, within GFF42 at 44.555 kb on - strand, within GFF42 at 44.555 kb on - strand, within GFF42 at 44.555 kb on - strand, within GFF42 at 44.640 kb on + strand at 44.640 kb on + strand at 44.641 kb on - strand at 44.641 kb on - strand at 44.641 kb on - strand at 44.682 kb on - strand at 44.682 kb on - strand at 44.702 kb on - strand at 44.702 kb on - strand at 44.702 kb on - strand at 44.709 kb on + strand at 44.710 kb on - strand at 44.723 kb on + strand at 44.798 kb on - strand at 44.798 kb on - strand at 44.802 kb on - strand at 44.802 kb on - strand at 44.802 kb on - strand at 44.811 kb on + strand at 44.812 kb on - strand at 44.881 kb on + strand at 44.881 kb on + strand at 44.881 kb on + strand at 44.881 kb on + strand at 44.882 kb on - strand at 44.882 kb on - strand at 44.882 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 42,784 + GFF40 0.29 -0.0 42,784 + GFF40 0.29 -0.0 42,970 + GFF40 0.46 -1.5 43,039 + GFF40 0.52 -1.0 43,039 + GFF40 0.52 -0.5 43,039 + GFF40 0.52 -1.5 43,039 + GFF40 0.52 +1.8 43,039 + GFF40 0.52 +0.6 43,039 + GFF40 0.52 -2.6 43,039 + GFF40 0.52 -0.4 43,039 + GFF40 0.52 +0.3 43,040 - GFF40 0.52 +0.3 43,040 - GFF40 0.52 -0.2 43,165 + GFF40 0.63 -1.5 43,166 - GFF40 0.63 -0.1 43,343 - GFF40 0.78 -1.2 43,343 - GFF40 0.78 -0.4 43,534 + +0.0 43,534 + +1.3 43,534 + -0.0 43,534 + +0.0 43,534 + -0.4 43,534 + -0.2 43,534 + -2.4 43,535 - -0.5 43,535 - +0.6 43,535 - -0.9 43,537 + -0.2 43,583 + -0.7 43,584 - +0.2 43,615 + -0.4 43,616 - -0.8 43,628 + +0.5 43,628 + -1.0 43,628 + +0.1 43,628 + -0.5 43,628 + -0.3 43,628 + +0.2 43,628 + -1.0 43,628 + +0.1 43,628 + +0.0 43,628 + -1.0 43,628 + +2.4 43,628 + +0.2 43,628 + -0.2 43,629 - +1.8 43,629 - +0.8 43,629 - -1.4 43,629 - -0.5 43,629 - +0.0 43,629 - +0.6 43,629 - -1.1 43,857 + GFF41 0.26 +0.3 43,857 + GFF41 0.26 -0.9 43,857 + GFF41 0.26 +0.9 43,857 + GFF41 0.26 -0.6 43,858 - GFF41 0.26 -1.2 43,885 + GFF41 0.35 +0.5 43,897 + GFF41 0.38 +0.8 44,085 + -0.3 44,085 + -1.2 44,085 + +0.3 44,086 - -0.7 44,141 + -0.9 44,142 - -1.0 44,142 - -0.3 44,197 + -0.4 44,197 + +0.4 44,197 + -0.1 44,197 + -0.2 44,197 + -0.7 44,197 + -1.2 44,198 - +0.1 44,198 - +0.0 44,198 - -0.2 44,198 - -0.1 44,198 - -0.1 44,198 - -0.5 44,198 - +0.0 44,269 - GFF42 0.23 +1.8 44,508 - GFF42 0.77 +0.5 44,554 + GFF42 0.87 -0.9 44,554 + GFF42 0.87 -0.1 44,554 + GFF42 0.87 +0.6 44,554 + GFF42 0.87 +0.9 44,554 + GFF42 0.87 -1.2 44,555 - GFF42 0.87 +0.5 44,555 - GFF42 0.87 +0.4 44,555 - GFF42 0.87 -2.2 44,555 - GFF42 0.87 -0.1 44,555 - GFF42 0.87 -0.8 44,555 - GFF42 0.87 -0.2 44,640 + -0.1 44,640 + -0.2 44,641 - -0.9 44,641 - -0.4 44,641 - -1.2 44,682 - +0.3 44,682 - +2.2 44,702 - +0.5 44,702 - -0.2 44,702 - +0.4 44,709 + -1.8 44,710 - -3.2 44,723 + -0.2 44,798 - -0.5 44,798 - -0.2 44,802 - -1.0 44,802 - -0.7 44,802 - +0.0 44,811 + -1.1 44,812 - -0.7 44,881 + -0.6 44,881 + +0.9 44,881 + +0.3 44,881 + -0.7 44,882 - -0.0 44,882 - +0.1 44,882 - -1.7
Or see this region's nucleotide sequence