Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3732 and GFF3733 are separated by 98 nucleotides GFF3733 and GFF3734 overlap by 4 nucleotides GFF3734 and GFF3735 overlap by 4 nucleotides GFF3735 and GFF3736 are separated by 46 nucleotides
GFF3732 - hypothetical protein, at 230,107 to 230,604
GFF3732
GFF3733 - no description, at 230,703 to 231,599
GFF3733
GFF3734 - RNA:NAD 2'-phosphotransferase, at 231,596 to 232,132
GFF3734
GFF3735 - 16S rRNA (cytidine(1402)-2'-O)-methyltransferase (EC 2.1.1.198), at 232,129 to 233,076
GFF3735
GFF3736 - UPF0102 protein YraN, at 233,123 to 233,491
GFF3736
Position (kb) 231
232
233 Strain fitness (log2 ratio) -2
-1
0
1
2 at 230.641 kb on + strand at 230.641 kb on + strand at 230.667 kb on + strand at 230.667 kb on + strand at 230.667 kb on + strand at 230.667 kb on + strand at 230.700 kb on + strand at 230.859 kb on + strand, within GFF3733 at 230.859 kb on + strand, within GFF3733 at 231.015 kb on + strand, within GFF3733 at 231.015 kb on + strand, within GFF3733 at 231.016 kb on - strand, within GFF3733 at 231.021 kb on + strand, within GFF3733 at 231.021 kb on + strand, within GFF3733 at 231.022 kb on - strand, within GFF3733 at 231.022 kb on - strand, within GFF3733 at 231.022 kb on - strand, within GFF3733 at 231.022 kb on - strand, within GFF3733 at 231.022 kb on - strand, within GFF3733 at 231.022 kb on - strand, within GFF3733 at 231.191 kb on + strand, within GFF3733 at 231.192 kb on - strand, within GFF3733 at 231.192 kb on - strand, within GFF3733 at 231.192 kb on - strand, within GFF3733 at 231.192 kb on - strand, within GFF3733 at 231.224 kb on + strand, within GFF3733 at 231.498 kb on + strand, within GFF3733 at 231.499 kb on - strand, within GFF3733 at 231.499 kb on - strand, within GFF3733 at 231.629 kb on + strand at 231.629 kb on + strand at 231.629 kb on + strand at 231.629 kb on + strand at 231.629 kb on + strand at 231.630 kb on - strand at 231.630 kb on - strand at 231.630 kb on - strand at 231.630 kb on - strand at 231.630 kb on - strand at 231.785 kb on + strand, within GFF3734 at 231.785 kb on + strand, within GFF3734 at 231.785 kb on + strand, within GFF3734 at 231.785 kb on + strand, within GFF3734 at 232.172 kb on + strand at 232.172 kb on + strand at 232.172 kb on + strand at 232.172 kb on + strand at 232.172 kb on + strand at 232.172 kb on + strand at 232.172 kb on + strand at 232.173 kb on - strand at 232.173 kb on - strand at 232.198 kb on + strand at 232.199 kb on - strand at 232.199 kb on - strand at 232.428 kb on + strand, within GFF3735 at 232.754 kb on + strand, within GFF3735 at 232.754 kb on + strand, within GFF3735 at 232.985 kb on + strand at 232.985 kb on + strand at 232.985 kb on + strand at 232.986 kb on - strand at 232.986 kb on - strand at 232.986 kb on - strand at 232.986 kb on - strand at 232.986 kb on - strand at 232.986 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 230,641 + +1.4 230,641 + -0.2 230,667 + -0.9 230,667 + +1.2 230,667 + -0.5 230,667 + -1.6 230,700 + +0.1 230,859 + GFF3733 0.17 -0.6 230,859 + GFF3733 0.17 -0.9 231,015 + GFF3733 0.35 +0.0 231,015 + GFF3733 0.35 +0.1 231,016 - GFF3733 0.35 -1.0 231,021 + GFF3733 0.35 -0.2 231,021 + GFF3733 0.35 -0.1 231,022 - GFF3733 0.36 +0.7 231,022 - GFF3733 0.36 -0.3 231,022 - GFF3733 0.36 +0.0 231,022 - GFF3733 0.36 -1.1 231,022 - GFF3733 0.36 +0.2 231,022 - GFF3733 0.36 -0.4 231,191 + GFF3733 0.54 -1.6 231,192 - GFF3733 0.55 -0.1 231,192 - GFF3733 0.55 -0.8 231,192 - GFF3733 0.55 +0.2 231,192 - GFF3733 0.55 +0.1 231,224 + GFF3733 0.58 +0.0 231,498 + GFF3733 0.89 +0.4 231,499 - GFF3733 0.89 -0.8 231,499 - GFF3733 0.89 -1.2 231,629 + -0.4 231,629 + -0.7 231,629 + -0.7 231,629 + -0.6 231,629 + -0.4 231,630 - -1.1 231,630 - -1.1 231,630 - -1.6 231,630 - +0.0 231,630 - -0.8 231,785 + GFF3734 0.35 -0.8 231,785 + GFF3734 0.35 +0.2 231,785 + GFF3734 0.35 +0.6 231,785 + GFF3734 0.35 -0.7 232,172 + +1.0 232,172 + +1.0 232,172 + -1.2 232,172 + +0.1 232,172 + -0.5 232,172 + +1.7 232,172 + -1.7 232,173 - +0.8 232,173 - -1.1 232,198 + +0.2 232,199 - -0.6 232,199 - -0.7 232,428 + GFF3735 0.32 -0.0 232,754 + GFF3735 0.66 -1.8 232,754 + GFF3735 0.66 +1.2 232,985 + -0.8 232,985 + -0.3 232,985 + -0.9 232,986 - +1.1 232,986 - +2.2 232,986 - +0.2 232,986 - +1.7 232,986 - +0.9 232,986 - -0.0
Or see this region's nucleotide sequence