Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3709 and GFF3710 are separated by 58 nucleotides GFF3710 and GFF3711 are separated by 14 nucleotides
GFF3709 - ABC transporter, substrate-binding protein (cluster 11, riboflavin/purine nucleoside/unknown), at 209,362 to 210,471
GFF3709
GFF3710 - Lactoylglutathione lyase and related lyases, at 210,530 to 210,916
GFF3710
GFF3711 - oxidoreductase, 2OG-Fe(II) oxygenase family, at 210,931 to 211,932
GFF3711
Position (kb) 210
211 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 209.741 kb on + strand, within GFF3709 at 209.741 kb on + strand, within GFF3709 at 209.742 kb on - strand, within GFF3709 at 209.873 kb on + strand, within GFF3709 at 209.873 kb on + strand, within GFF3709 at 209.874 kb on - strand, within GFF3709 at 209.874 kb on - strand, within GFF3709 at 210.023 kb on + strand, within GFF3709 at 210.023 kb on + strand, within GFF3709 at 210.024 kb on - strand, within GFF3709 at 210.047 kb on + strand, within GFF3709 at 210.110 kb on + strand, within GFF3709 at 210.332 kb on + strand, within GFF3709 at 210.500 kb on + strand at 210.501 kb on - strand at 210.531 kb on + strand at 210.532 kb on - strand at 210.597 kb on + strand, within GFF3710 at 210.597 kb on + strand, within GFF3710 at 210.597 kb on + strand, within GFF3710 at 210.597 kb on + strand, within GFF3710 at 210.598 kb on - strand, within GFF3710 at 210.598 kb on - strand, within GFF3710 at 210.598 kb on - strand, within GFF3710 at 210.598 kb on - strand, within GFF3710 at 210.601 kb on - strand, within GFF3710 at 210.610 kb on - strand, within GFF3710 at 210.750 kb on + strand, within GFF3710 at 210.750 kb on + strand, within GFF3710 at 210.751 kb on - strand, within GFF3710 at 210.900 kb on + strand at 210.901 kb on - strand at 210.901 kb on - strand at 210.901 kb on - strand at 211.014 kb on - strand at 211.070 kb on + strand, within GFF3711 at 211.070 kb on + strand, within GFF3711 at 211.494 kb on - strand, within GFF3711 at 211.502 kb on + strand, within GFF3711 at 211.502 kb on + strand, within GFF3711 at 211.502 kb on + strand, within GFF3711 at 211.781 kb on - strand, within GFF3711 at 211.892 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 209,741 + GFF3709 0.34 -0.6 209,741 + GFF3709 0.34 +0.4 209,742 - GFF3709 0.34 +0.7 209,873 + GFF3709 0.46 -0.2 209,873 + GFF3709 0.46 -1.1 209,874 - GFF3709 0.46 +0.9 209,874 - GFF3709 0.46 -0.2 210,023 + GFF3709 0.60 +0.3 210,023 + GFF3709 0.60 -0.1 210,024 - GFF3709 0.60 +0.0 210,047 + GFF3709 0.62 -0.0 210,110 + GFF3709 0.67 -0.1 210,332 + GFF3709 0.87 -0.4 210,500 + +0.7 210,501 - -0.0 210,531 + +0.4 210,532 - -0.3 210,597 + GFF3710 0.17 -0.4 210,597 + GFF3710 0.17 +0.2 210,597 + GFF3710 0.17 -0.8 210,597 + GFF3710 0.17 +2.2 210,598 - GFF3710 0.18 -3.8 210,598 - GFF3710 0.18 -0.4 210,598 - GFF3710 0.18 +0.8 210,598 - GFF3710 0.18 -0.7 210,601 - GFF3710 0.18 -0.7 210,610 - GFF3710 0.21 +0.9 210,750 + GFF3710 0.57 +0.3 210,750 + GFF3710 0.57 -1.2 210,751 - GFF3710 0.57 +0.2 210,900 + +1.1 210,901 - +0.7 210,901 - -0.2 210,901 - -1.6 211,014 - -2.0 211,070 + GFF3711 0.14 -1.6 211,070 + GFF3711 0.14 +0.4 211,494 - GFF3711 0.56 -0.5 211,502 + GFF3711 0.57 -0.8 211,502 + GFF3711 0.57 +0.1 211,502 + GFF3711 0.57 -1.1 211,781 - GFF3711 0.85 -0.7 211,892 + -1.5
Or see this region's nucleotide sequence