Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3441 and GFF3442 are separated by 86 nucleotides GFF3442 and GFF3443 are separated by 6 nucleotides GFF3443 and GFF3444 overlap by 1 nucleotides
GFF3441 - Hypothetical protein VC0266 (sugar utilization related?), at 264,509 to 265,087
GFF3441
GFF3442 - RidA/YER057c/UK114 superfamily protein, at 265,174 to 265,578
GFF3442
GFF3443 - hypothetical protein, at 265,585 to 267,348
GFF3443
GFF3444 - BUG/TctC family periplasmic protein, at 267,348 to 268,337
GFF3444
Position (kb)
265
266
267
268 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 264.722 kb on + strand, within GFF3441 at 264.723 kb on - strand, within GFF3441 at 264.723 kb on - strand, within GFF3441 at 264.723 kb on - strand, within GFF3441 at 264.723 kb on - strand, within GFF3441 at 264.723 kb on - strand, within GFF3441 at 264.758 kb on + strand, within GFF3441 at 264.759 kb on - strand, within GFF3441 at 264.759 kb on - strand, within GFF3441 at 264.873 kb on + strand, within GFF3441 at 264.873 kb on + strand, within GFF3441 at 264.874 kb on - strand, within GFF3441 at 264.874 kb on - strand, within GFF3441 at 264.882 kb on - strand, within GFF3441 at 264.954 kb on - strand, within GFF3441 at 265.097 kb on + strand at 265.098 kb on - strand at 265.098 kb on - strand at 265.098 kb on - strand at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.423 kb on + strand, within GFF3442 at 265.423 kb on + strand, within GFF3442 at 265.424 kb on - strand, within GFF3442 at 265.424 kb on - strand, within GFF3442 at 265.424 kb on - strand, within GFF3442 at 265.424 kb on - strand, within GFF3442 at 265.424 kb on - strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.466 kb on - strand, within GFF3442 at 265.466 kb on - strand, within GFF3442 at 265.466 kb on - strand, within GFF3442 at 265.466 kb on - strand, within GFF3442 at 265.466 kb on - strand, within GFF3442 at 265.637 kb on + strand at 265.637 kb on + strand at 265.638 kb on - strand at 265.638 kb on - strand at 265.638 kb on - strand at 265.638 kb on - strand at 265.638 kb on - strand at 265.806 kb on - strand, within GFF3443 at 265.913 kb on + strand, within GFF3443 at 265.913 kb on + strand, within GFF3443 at 265.914 kb on - strand, within GFF3443 at 265.914 kb on - strand, within GFF3443 at 265.914 kb on - strand, within GFF3443 at 265.914 kb on - strand, within GFF3443 at 265.914 kb on - strand, within GFF3443 at 265.914 kb on - strand, within GFF3443 at 265.914 kb on - strand, within GFF3443 at 266.039 kb on + strand, within GFF3443 at 266.039 kb on + strand, within GFF3443 at 266.039 kb on + strand, within GFF3443 at 266.040 kb on - strand, within GFF3443 at 266.040 kb on - strand, within GFF3443 at 266.040 kb on - strand, within GFF3443 at 266.261 kb on + strand, within GFF3443 at 266.261 kb on + strand, within GFF3443 at 266.261 kb on + strand, within GFF3443 at 266.262 kb on - strand, within GFF3443 at 266.303 kb on + strand, within GFF3443 at 266.303 kb on + strand, within GFF3443 at 266.303 kb on + strand, within GFF3443 at 266.304 kb on - strand, within GFF3443 at 266.304 kb on - strand, within GFF3443 at 266.304 kb on - strand, within GFF3443 at 266.436 kb on - strand, within GFF3443 at 266.436 kb on - strand, within GFF3443 at 266.594 kb on + strand, within GFF3443 at 266.594 kb on + strand, within GFF3443 at 266.594 kb on + strand, within GFF3443 at 266.595 kb on - strand, within GFF3443 at 266.595 kb on - strand, within GFF3443 at 266.595 kb on - strand, within GFF3443 at 266.743 kb on + strand, within GFF3443 at 266.743 kb on + strand, within GFF3443 at 266.756 kb on + strand, within GFF3443 at 266.756 kb on + strand, within GFF3443 at 266.771 kb on + strand, within GFF3443 at 266.771 kb on + strand, within GFF3443 at 266.771 kb on + strand, within GFF3443 at 266.771 kb on + strand, within GFF3443 at 266.772 kb on - strand, within GFF3443 at 266.772 kb on - strand, within GFF3443 at 266.772 kb on - strand, within GFF3443 at 266.772 kb on - strand, within GFF3443 at 266.963 kb on + strand, within GFF3443 at 267.577 kb on + strand, within GFF3444 at 267.577 kb on + strand, within GFF3444 at 267.578 kb on - strand, within GFF3444 at 267.578 kb on - strand, within GFF3444 at 267.856 kb on + strand, within GFF3444 at 267.856 kb on + strand, within GFF3444 at 267.856 kb on + strand, within GFF3444 at 267.857 kb on - strand, within GFF3444 at 267.857 kb on - strand, within GFF3444 at 267.992 kb on - strand, within GFF3444 at 268.007 kb on - strand, within GFF3444 at 268.054 kb on + strand, within GFF3444
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 264,722 + GFF3441 0.37 -0.4 264,723 - GFF3441 0.37 +1.7 264,723 - GFF3441 0.37 -1.2 264,723 - GFF3441 0.37 +0.2 264,723 - GFF3441 0.37 -0.6 264,723 - GFF3441 0.37 +0.1 264,758 + GFF3441 0.43 -0.3 264,759 - GFF3441 0.43 -0.8 264,759 - GFF3441 0.43 -0.6 264,873 + GFF3441 0.63 -1.0 264,873 + GFF3441 0.63 -2.2 264,874 - GFF3441 0.63 -0.9 264,874 - GFF3441 0.63 -1.3 264,882 - GFF3441 0.64 +0.8 264,954 - GFF3441 0.77 +1.3 265,097 + +2.4 265,098 - +0.8 265,098 - +0.5 265,098 - -1.0 265,270 + GFF3442 0.24 +0.3 265,270 + GFF3442 0.24 +0.2 265,270 + GFF3442 0.24 -0.4 265,270 + GFF3442 0.24 -0.3 265,270 + GFF3442 0.24 -0.2 265,270 + GFF3442 0.24 -1.2 265,270 + GFF3442 0.24 +1.9 265,270 + GFF3442 0.24 -0.5 265,270 + GFF3442 0.24 -0.3 265,270 + GFF3442 0.24 +0.5 265,271 - GFF3442 0.24 +1.5 265,271 - GFF3442 0.24 -0.1 265,271 - GFF3442 0.24 +0.5 265,271 - GFF3442 0.24 +1.0 265,271 - GFF3442 0.24 -0.9 265,271 - GFF3442 0.24 -0.5 265,271 - GFF3442 0.24 -1.3 265,271 - GFF3442 0.24 +0.4 265,271 - GFF3442 0.24 +0.0 265,423 + GFF3442 0.61 +2.1 265,423 + GFF3442 0.61 -0.1 265,424 - GFF3442 0.62 -0.5 265,424 - GFF3442 0.62 -0.2 265,424 - GFF3442 0.62 -0.1 265,424 - GFF3442 0.62 -0.5 265,424 - GFF3442 0.62 +0.4 265,465 + GFF3442 0.72 +0.8 265,465 + GFF3442 0.72 -0.8 265,465 + GFF3442 0.72 -0.6 265,465 + GFF3442 0.72 -1.6 265,465 + GFF3442 0.72 +0.9 265,465 + GFF3442 0.72 -0.2 265,465 + GFF3442 0.72 -1.1 265,465 + GFF3442 0.72 -0.7 265,465 + GFF3442 0.72 +0.6 265,465 + GFF3442 0.72 -0.2 265,466 - GFF3442 0.72 +0.8 265,466 - GFF3442 0.72 -0.2 265,466 - GFF3442 0.72 +1.0 265,466 - GFF3442 0.72 +0.4 265,466 - GFF3442 0.72 -0.4 265,637 + -0.1 265,637 + -0.2 265,638 - -2.5 265,638 - -1.3 265,638 - -0.1 265,638 - -0.9 265,638 - -0.2 265,806 - GFF3443 0.13 +0.3 265,913 + GFF3443 0.19 -0.3 265,913 + GFF3443 0.19 -0.6 265,914 - GFF3443 0.19 +0.4 265,914 - GFF3443 0.19 +2.9 265,914 - GFF3443 0.19 -0.4 265,914 - GFF3443 0.19 -0.3 265,914 - GFF3443 0.19 -0.6 265,914 - GFF3443 0.19 -0.0 265,914 - GFF3443 0.19 -1.4 266,039 + GFF3443 0.26 +0.8 266,039 + GFF3443 0.26 +0.0 266,039 + GFF3443 0.26 -0.5 266,040 - GFF3443 0.26 -0.9 266,040 - GFF3443 0.26 -0.8 266,040 - GFF3443 0.26 -1.0 266,261 + GFF3443 0.38 +0.4 266,261 + GFF3443 0.38 +0.2 266,261 + GFF3443 0.38 -1.5 266,262 - GFF3443 0.38 +1.2 266,303 + GFF3443 0.41 +1.5 266,303 + GFF3443 0.41 +3.4 266,303 + GFF3443 0.41 -0.4 266,304 - GFF3443 0.41 -0.8 266,304 - GFF3443 0.41 -0.2 266,304 - GFF3443 0.41 -1.6 266,436 - GFF3443 0.48 +0.4 266,436 - GFF3443 0.48 -0.3 266,594 + GFF3443 0.57 -0.9 266,594 + GFF3443 0.57 +0.1 266,594 + GFF3443 0.57 -0.3 266,595 - GFF3443 0.57 +0.1 266,595 - GFF3443 0.57 +1.4 266,595 - GFF3443 0.57 +0.8 266,743 + GFF3443 0.66 -0.5 266,743 + GFF3443 0.66 -1.6 266,756 + GFF3443 0.66 +0.2 266,756 + GFF3443 0.66 -1.8 266,771 + GFF3443 0.67 +0.8 266,771 + GFF3443 0.67 -0.9 266,771 + GFF3443 0.67 -1.6 266,771 + GFF3443 0.67 -0.4 266,772 - GFF3443 0.67 -1.5 266,772 - GFF3443 0.67 -1.7 266,772 - GFF3443 0.67 -0.2 266,772 - GFF3443 0.67 -1.1 266,963 + GFF3443 0.78 +0.0 267,577 + GFF3444 0.23 -0.5 267,577 + GFF3444 0.23 -0.7 267,578 - GFF3444 0.23 +1.4 267,578 - GFF3444 0.23 +0.8 267,856 + GFF3444 0.51 +0.4 267,856 + GFF3444 0.51 +0.1 267,856 + GFF3444 0.51 +0.5 267,857 - GFF3444 0.51 -0.7 267,857 - GFF3444 0.51 -0.8 267,992 - GFF3444 0.65 -0.4 268,007 - GFF3444 0.67 +0.3 268,054 + GFF3444 0.71 -0.4
Or see this region's nucleotide sequence