Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3302 and GFF3303 overlap by 4 nucleotides GFF3303 and GFF3304 overlap by 4 nucleotides GFF3304 and GFF3305 are separated by 11 nucleotides GFF3305 and GFF3306 overlap by 20 nucleotides
GFF3302 - no description, at 126,376 to 127,017
GFF3302
GFF3303 - Acetyltransferase, GNAT family, at 127,014 to 127,778
GFF3303
GFF3304 - Monofunctional biosynthetic peptidoglycan transglycosylase, at 127,775 to 129,043
GFF3304
GFF3305 - hypothetical protein, at 129,055 to 129,624
GFF3305
GFF3306 - hypothetical protein, at 129,605 to 130,534
GFF3306
Position (kb)
127
128
129
130 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 126.880 kb on + strand, within GFF3302 at 126.880 kb on + strand, within GFF3302 at 126.880 kb on + strand, within GFF3302 at 126.880 kb on + strand, within GFF3302 at 126.880 kb on + strand, within GFF3302 at 126.880 kb on + strand, within GFF3302 at 126.880 kb on + strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 127.015 kb on + strand at 127.016 kb on - strand at 127.037 kb on - strand at 127.069 kb on + strand at 127.069 kb on + strand at 127.069 kb on + strand at 127.069 kb on + strand at 127.069 kb on + strand at 127.069 kb on + strand at 127.070 kb on - strand at 127.070 kb on - strand at 127.070 kb on - strand at 127.070 kb on - strand at 127.070 kb on - strand at 127.070 kb on - strand at 127.263 kb on - strand, within GFF3303 at 127.316 kb on + strand, within GFF3303 at 127.317 kb on - strand, within GFF3303 at 127.317 kb on - strand, within GFF3303 at 127.317 kb on - strand, within GFF3303 at 127.317 kb on - strand, within GFF3303 at 127.487 kb on - strand, within GFF3303 at 127.487 kb on - strand, within GFF3303 at 127.487 kb on - strand, within GFF3303 at 127.744 kb on - strand at 128.007 kb on + strand, within GFF3304 at 128.008 kb on - strand, within GFF3304 at 128.067 kb on + strand, within GFF3304 at 128.067 kb on + strand, within GFF3304 at 128.067 kb on + strand, within GFF3304 at 128.068 kb on - strand, within GFF3304 at 128.068 kb on - strand, within GFF3304 at 128.068 kb on - strand, within GFF3304 at 128.068 kb on - strand, within GFF3304 at 128.119 kb on - strand, within GFF3304 at 128.119 kb on - strand, within GFF3304 at 128.119 kb on - strand, within GFF3304 at 128.269 kb on - strand, within GFF3304 at 128.532 kb on + strand, within GFF3304 at 128.841 kb on + strand, within GFF3304 at 128.841 kb on + strand, within GFF3304 at 128.841 kb on + strand, within GFF3304 at 128.841 kb on + strand, within GFF3304 at 128.841 kb on + strand, within GFF3304 at 128.841 kb on + strand, within GFF3304 at 128.842 kb on - strand, within GFF3304 at 128.842 kb on - strand, within GFF3304 at 128.842 kb on - strand, within GFF3304 at 128.978 kb on - strand at 128.983 kb on + strand at 128.983 kb on + strand at 128.984 kb on - strand at 128.984 kb on - strand at 128.984 kb on - strand at 128.984 kb on - strand at 129.025 kb on + strand at 129.025 kb on + strand at 129.025 kb on + strand at 129.025 kb on + strand at 129.026 kb on - strand at 129.026 kb on - strand at 129.026 kb on - strand at 129.026 kb on - strand at 129.122 kb on + strand, within GFF3305 at 129.122 kb on + strand, within GFF3305 at 129.122 kb on + strand, within GFF3305 at 129.123 kb on - strand, within GFF3305 at 129.123 kb on - strand, within GFF3305 at 129.123 kb on - strand, within GFF3305 at 129.273 kb on - strand, within GFF3305 at 129.287 kb on + strand, within GFF3305
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 126,880 + GFF3302 0.79 -1.2 126,880 + GFF3302 0.79 -0.2 126,880 + GFF3302 0.79 -0.9 126,880 + GFF3302 0.79 -2.5 126,880 + GFF3302 0.79 -0.9 126,880 + GFF3302 0.79 -2.0 126,880 + GFF3302 0.79 -1.6 126,881 - GFF3302 0.79 -0.6 126,881 - GFF3302 0.79 -1.1 126,881 - GFF3302 0.79 -0.8 126,881 - GFF3302 0.79 -0.4 126,881 - GFF3302 0.79 +0.8 126,881 - GFF3302 0.79 +0.9 126,881 - GFF3302 0.79 -2.7 126,881 - GFF3302 0.79 -0.4 126,881 - GFF3302 0.79 +2.0 126,881 - GFF3302 0.79 +0.0 126,881 - GFF3302 0.79 -1.0 126,881 - GFF3302 0.79 -0.1 126,881 - GFF3302 0.79 -1.0 127,015 + -0.3 127,016 - -1.2 127,037 - -0.1 127,069 + -0.6 127,069 + -1.3 127,069 + -0.9 127,069 + +0.1 127,069 + +1.4 127,069 + -1.0 127,070 - -1.3 127,070 - -0.4 127,070 - -0.5 127,070 - -2.5 127,070 - -0.4 127,070 - -0.5 127,263 - GFF3303 0.33 +1.2 127,316 + GFF3303 0.39 -2.0 127,317 - GFF3303 0.40 +0.5 127,317 - GFF3303 0.40 -3.5 127,317 - GFF3303 0.40 +0.0 127,317 - GFF3303 0.40 +0.6 127,487 - GFF3303 0.62 +0.5 127,487 - GFF3303 0.62 -0.1 127,487 - GFF3303 0.62 +0.9 127,744 - -3.6 128,007 + GFF3304 0.18 +1.2 128,008 - GFF3304 0.18 -0.1 128,067 + GFF3304 0.23 -0.8 128,067 + GFF3304 0.23 -0.4 128,067 + GFF3304 0.23 -0.4 128,068 - GFF3304 0.23 -0.5 128,068 - GFF3304 0.23 -0.3 128,068 - GFF3304 0.23 -0.0 128,068 - GFF3304 0.23 -1.2 128,119 - GFF3304 0.27 +0.2 128,119 - GFF3304 0.27 -0.5 128,119 - GFF3304 0.27 -1.3 128,269 - GFF3304 0.39 +0.2 128,532 + GFF3304 0.60 +1.4 128,841 + GFF3304 0.84 -1.0 128,841 + GFF3304 0.84 +0.2 128,841 + GFF3304 0.84 +1.4 128,841 + GFF3304 0.84 -0.3 128,841 + GFF3304 0.84 -0.7 128,841 + GFF3304 0.84 -0.3 128,842 - GFF3304 0.84 -1.3 128,842 - GFF3304 0.84 -1.7 128,842 - GFF3304 0.84 +0.5 128,978 - -0.2 128,983 + +0.3 128,983 + +0.5 128,984 - +0.5 128,984 - -1.5 128,984 - +1.0 128,984 - -0.1 129,025 + +0.4 129,025 + -0.4 129,025 + +1.0 129,025 + +0.0 129,026 - -0.6 129,026 - -0.7 129,026 - -1.0 129,026 - -0.5 129,122 + GFF3305 0.12 -0.2 129,122 + GFF3305 0.12 -1.4 129,122 + GFF3305 0.12 -0.2 129,123 - GFF3305 0.12 -0.2 129,123 - GFF3305 0.12 -0.4 129,123 - GFF3305 0.12 +0.9 129,273 - GFF3305 0.38 -0.6 129,287 + GFF3305 0.41 -0.2
Or see this region's nucleotide sequence