Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3236 and GFF3237 overlap by 4 nucleotides GFF3237 and GFF3238 overlap by 4 nucleotides GFF3238 and GFF3239 overlap by 4 nucleotides GFF3239 and GFF3240 are separated by 11 nucleotides GFF3240 and GFF3241 overlap by 4 nucleotides
GFF3236 - no description, at 53,175 to 53,573
GFF3236
GFF3237 - no description, at 53,570 to 53,809
GFF3237
GFF3238 - no description, at 53,806 to 54,216
GFF3238
GFF3239 - no description, at 54,213 to 54,605
GFF3239
GFF3240 - hypothetical protein, at 54,617 to 55,237
GFF3240
GFF3241 - hypothetical protein, at 55,234 to 55,698
GFF3241
Position (kb) 54
55 Strain fitness (log2 ratio) -1
0
1
2
3 at 53.514 kb on + strand, within GFF3236 at 53.591 kb on + strand at 53.591 kb on + strand at 53.591 kb on + strand at 53.591 kb on + strand at 53.591 kb on + strand at 53.592 kb on - strand at 53.592 kb on - strand at 53.592 kb on - strand at 53.592 kb on - strand at 53.592 kb on - strand at 53.717 kb on + strand, within GFF3237 at 53.958 kb on + strand, within GFF3238 at 53.959 kb on - strand, within GFF3238 at 54.109 kb on + strand, within GFF3238 at 54.109 kb on + strand, within GFF3238 at 54.109 kb on + strand, within GFF3238 at 54.109 kb on + strand, within GFF3238 at 54.110 kb on - strand, within GFF3238 at 54.110 kb on - strand, within GFF3238 at 54.196 kb on + strand at 54.197 kb on - strand at 54.426 kb on + strand, within GFF3239 at 54.426 kb on + strand, within GFF3239 at 54.427 kb on - strand, within GFF3239 at 54.429 kb on + strand, within GFF3239 at 54.472 kb on - strand, within GFF3239 at 54.472 kb on - strand, within GFF3239 at 54.830 kb on + strand, within GFF3240 at 54.830 kb on + strand, within GFF3240 at 55.143 kb on - strand, within GFF3240 at 55.163 kb on + strand, within GFF3240 at 55.163 kb on + strand, within GFF3240 at 55.163 kb on + strand, within GFF3240 at 55.163 kb on + strand, within GFF3240 at 55.164 kb on - strand, within GFF3240 at 55.164 kb on - strand, within GFF3240 at 55.164 kb on - strand, within GFF3240 at 55.176 kb on - strand at 55.202 kb on + strand at 55.234 kb on - strand at 55.436 kb on - strand, within GFF3241 at 55.436 kb on - strand, within GFF3241 at 55.436 kb on - strand, within GFF3241 at 55.579 kb on - strand, within GFF3241
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 53,514 + GFF3236 0.85 +1.4 53,591 + -0.1 53,591 + +1.9 53,591 + +1.9 53,591 + -0.7 53,591 + +1.4 53,592 - -1.3 53,592 - -0.4 53,592 - -1.6 53,592 - -0.6 53,592 - -1.4 53,717 + GFF3237 0.61 +0.7 53,958 + GFF3238 0.37 +0.8 53,959 - GFF3238 0.37 +0.9 54,109 + GFF3238 0.74 -0.2 54,109 + GFF3238 0.74 -0.9 54,109 + GFF3238 0.74 -1.0 54,109 + GFF3238 0.74 -0.4 54,110 - GFF3238 0.74 -1.5 54,110 - GFF3238 0.74 +1.8 54,196 + -1.0 54,197 - +2.0 54,426 + GFF3239 0.54 -1.2 54,426 + GFF3239 0.54 -0.4 54,427 - GFF3239 0.54 -1.5 54,429 + GFF3239 0.55 -1.1 54,472 - GFF3239 0.66 -0.2 54,472 - GFF3239 0.66 -0.6 54,830 + GFF3240 0.34 -0.5 54,830 + GFF3240 0.34 +0.5 55,143 - GFF3240 0.85 +0.4 55,163 + GFF3240 0.88 -0.2 55,163 + GFF3240 0.88 -0.7 55,163 + GFF3240 0.88 +1.1 55,163 + GFF3240 0.88 -1.6 55,164 - GFF3240 0.88 +1.8 55,164 - GFF3240 0.88 -0.2 55,164 - GFF3240 0.88 +3.4 55,176 - -0.2 55,202 + -0.5 55,234 - -1.7 55,436 - GFF3241 0.43 +0.6 55,436 - GFF3241 0.43 -0.9 55,436 - GFF3241 0.43 +0.3 55,579 - GFF3241 0.74 +0.8
Or see this region's nucleotide sequence