Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3149 and GFF3150 are separated by 101 nucleotides GFF3150 and GFF3151 are separated by 82 nucleotides GFF3151 and GFF3152 are separated by 27 nucleotides
GFF3149 - RND efflux system, inner membrane transporter, at 583,099 to 586,152
GFF3149
GFF3150 - Transcriptional regulator, AsnC family, at 586,254 to 586,784
GFF3150
GFF3151 - no description, at 586,867 to 587,691
GFF3151
GFF3152 - hypothetical protein, at 587,719 to 589,104
GFF3152
Position (kb) 586
587
588 Strain fitness (log2 ratio) -2
-1
0
1
2 at 585.887 kb on - strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.126 kb on + strand at 586.127 kb on - strand at 586.205 kb on + strand at 586.205 kb on + strand at 586.205 kb on + strand at 586.205 kb on + strand at 586.205 kb on + strand at 586.205 kb on + strand at 586.205 kb on + strand at 586.205 kb on + strand at 586.205 kb on + strand at 586.205 kb on + strand at 586.205 kb on + strand at 586.206 kb on - strand at 586.206 kb on - strand at 586.206 kb on - strand at 586.206 kb on - strand at 586.206 kb on - strand at 586.206 kb on - strand at 586.206 kb on - strand at 586.206 kb on - strand at 586.210 kb on + strand at 586.211 kb on - strand at 586.211 kb on - strand at 586.422 kb on + strand, within GFF3150 at 586.422 kb on + strand, within GFF3150 at 586.584 kb on + strand, within GFF3150 at 586.584 kb on + strand, within GFF3150 at 586.585 kb on - strand, within GFF3150 at 586.585 kb on - strand, within GFF3150 at 586.585 kb on - strand, within GFF3150 at 586.585 kb on - strand, within GFF3150 at 586.621 kb on - strand, within GFF3150 at 586.782 kb on + strand at 586.783 kb on - strand at 586.783 kb on - strand at 587.230 kb on + strand, within GFF3151 at 587.230 kb on + strand, within GFF3151 at 587.230 kb on + strand, within GFF3151 at 587.231 kb on - strand, within GFF3151 at 587.231 kb on - strand, within GFF3151 at 587.231 kb on - strand, within GFF3151 at 587.231 kb on - strand, within GFF3151 at 587.231 kb on - strand, within GFF3151 at 587.231 kb on - strand, within GFF3151 at 587.374 kb on + strand, within GFF3151 at 587.399 kb on - strand, within GFF3151 at 587.399 kb on - strand, within GFF3151 at 587.537 kb on - strand, within GFF3151 at 587.537 kb on - strand, within GFF3151 at 587.689 kb on + strand at 587.689 kb on + strand at 587.689 kb on + strand at 587.690 kb on - strand at 588.014 kb on + strand, within GFF3152 at 588.015 kb on - strand, within GFF3152 at 588.358 kb on - strand, within GFF3152 at 588.358 kb on - strand, within GFF3152
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 585,887 - -0.4 586,126 + +0.0 586,126 + +0.4 586,126 + -0.6 586,126 + -2.0 586,127 - -0.1 586,205 + -0.1 586,205 + -0.8 586,205 + -0.2 586,205 + -1.9 586,205 + -0.8 586,205 + -1.1 586,205 + -1.3 586,205 + +0.1 586,205 + +0.2 586,205 + -0.9 586,205 + -1.6 586,206 - +0.2 586,206 - +0.7 586,206 - +2.0 586,206 - -0.3 586,206 - -0.6 586,206 - +0.5 586,206 - -0.6 586,206 - -1.1 586,210 + +0.0 586,211 - +1.2 586,211 - -0.5 586,422 + GFF3150 0.32 -0.3 586,422 + GFF3150 0.32 -0.1 586,584 + GFF3150 0.62 -0.1 586,584 + GFF3150 0.62 +0.0 586,585 - GFF3150 0.62 -0.2 586,585 - GFF3150 0.62 +2.5 586,585 - GFF3150 0.62 -1.8 586,585 - GFF3150 0.62 +0.1 586,621 - GFF3150 0.69 -1.3 586,782 + +2.3 586,783 - -1.5 586,783 - -1.4 587,230 + GFF3151 0.44 -0.9 587,230 + GFF3151 0.44 -1.4 587,230 + GFF3151 0.44 -0.5 587,231 - GFF3151 0.44 -0.7 587,231 - GFF3151 0.44 +0.2 587,231 - GFF3151 0.44 -2.1 587,231 - GFF3151 0.44 -0.1 587,231 - GFF3151 0.44 -0.5 587,231 - GFF3151 0.44 -1.0 587,374 + GFF3151 0.61 -0.8 587,399 - GFF3151 0.64 -0.5 587,399 - GFF3151 0.64 +0.2 587,537 - GFF3151 0.81 -0.4 587,537 - GFF3151 0.81 -1.4 587,689 + -0.9 587,689 + -1.1 587,689 + -0.3 587,690 - -0.6 588,014 + GFF3152 0.21 -0.7 588,015 - GFF3152 0.21 -1.1 588,358 - GFF3152 0.46 -0.8 588,358 - GFF3152 0.46 +1.8
Or see this region's nucleotide sequence