Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3143 and GFF3144 are separated by 49 nucleotides GFF3144 and GFF3145 are separated by 2 nucleotides GFF3145 and GFF3146 are separated by 43 nucleotides
GFF3143 - hypothetical protein, at 576,947 to 577,816
GFF3143
GFF3144 - hypothetical protein, at 577,866 to 579,218
GFF3144
GFF3145 - Two-component system response regulator QseB, at 579,221 to 579,847
GFF3145
GFF3146 - no description, at 579,891 to 580,481
GFF3146
Position (kb) 577
578
579
580 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 576.913 kb on + strand at 576.914 kb on - strand at 577.021 kb on - strand at 577.038 kb on + strand, within GFF3143 at 577.101 kb on + strand, within GFF3143 at 577.102 kb on - strand, within GFF3143 at 577.102 kb on - strand, within GFF3143 at 577.147 kb on - strand, within GFF3143 at 577.218 kb on + strand, within GFF3143 at 577.219 kb on - strand, within GFF3143 at 577.263 kb on + strand, within GFF3143 at 577.263 kb on + strand, within GFF3143 at 577.264 kb on - strand, within GFF3143 at 577.324 kb on - strand, within GFF3143 at 577.324 kb on - strand, within GFF3143 at 577.443 kb on + strand, within GFF3143 at 577.519 kb on + strand, within GFF3143 at 577.519 kb on + strand, within GFF3143 at 577.519 kb on + strand, within GFF3143 at 577.602 kb on + strand, within GFF3143 at 577.602 kb on + strand, within GFF3143 at 577.602 kb on + strand, within GFF3143 at 577.602 kb on + strand, within GFF3143 at 577.603 kb on - strand, within GFF3143 at 577.603 kb on - strand, within GFF3143 at 577.603 kb on - strand, within GFF3143 at 577.603 kb on - strand, within GFF3143 at 577.603 kb on - strand, within GFF3143 at 577.603 kb on - strand, within GFF3143 at 577.603 kb on - strand, within GFF3143 at 577.691 kb on + strand, within GFF3143 at 577.691 kb on + strand, within GFF3143 at 577.844 kb on + strand at 577.844 kb on + strand at 577.845 kb on - strand at 577.845 kb on - strand at 577.845 kb on - strand at 578.158 kb on + strand, within GFF3144 at 578.159 kb on - strand, within GFF3144 at 578.159 kb on - strand, within GFF3144 at 578.929 kb on + strand, within GFF3144 at 578.930 kb on - strand, within GFF3144 at 579.136 kb on + strand at 579.136 kb on + strand at 579.137 kb on - strand at 579.137 kb on - strand at 579.137 kb on - strand at 579.292 kb on - strand, within GFF3145 at 579.343 kb on - strand, within GFF3145 at 579.349 kb on - strand, within GFF3145 at 579.585 kb on + strand, within GFF3145 at 579.585 kb on + strand, within GFF3145 at 579.585 kb on + strand, within GFF3145 at 579.866 kb on + strand at 579.866 kb on + strand at 579.938 kb on - strand at 580.210 kb on + strand, within GFF3146 at 580.210 kb on + strand, within GFF3146 at 580.210 kb on + strand, within GFF3146 at 580.211 kb on - strand, within GFF3146
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 576,913 + -0.1 576,914 - +0.3 577,021 - -0.5 577,038 + GFF3143 0.10 +0.6 577,101 + GFF3143 0.18 -1.4 577,102 - GFF3143 0.18 -0.3 577,102 - GFF3143 0.18 -0.2 577,147 - GFF3143 0.23 +0.7 577,218 + GFF3143 0.31 -0.2 577,219 - GFF3143 0.31 -0.8 577,263 + GFF3143 0.36 -0.1 577,263 + GFF3143 0.36 -0.9 577,264 - GFF3143 0.36 +2.0 577,324 - GFF3143 0.43 -0.1 577,324 - GFF3143 0.43 -1.2 577,443 + GFF3143 0.57 +1.3 577,519 + GFF3143 0.66 -0.5 577,519 + GFF3143 0.66 -0.2 577,519 + GFF3143 0.66 +0.0 577,602 + GFF3143 0.75 -0.8 577,602 + GFF3143 0.75 -0.1 577,602 + GFF3143 0.75 -0.2 577,602 + GFF3143 0.75 -0.3 577,603 - GFF3143 0.75 -1.1 577,603 - GFF3143 0.75 -0.2 577,603 - GFF3143 0.75 +1.0 577,603 - GFF3143 0.75 +0.5 577,603 - GFF3143 0.75 +0.0 577,603 - GFF3143 0.75 -2.0 577,603 - GFF3143 0.75 -0.4 577,691 + GFF3143 0.86 -2.5 577,691 + GFF3143 0.86 -2.9 577,844 + -0.5 577,844 + +0.4 577,845 - -1.1 577,845 - -0.4 577,845 - -0.8 578,158 + GFF3144 0.22 -1.3 578,159 - GFF3144 0.22 -1.1 578,159 - GFF3144 0.22 -0.1 578,929 + GFF3144 0.79 -0.4 578,930 - GFF3144 0.79 -0.6 579,136 + -0.9 579,136 + -0.8 579,137 - +1.0 579,137 - -0.0 579,137 - +0.0 579,292 - GFF3145 0.11 -0.4 579,343 - GFF3145 0.19 +0.2 579,349 - GFF3145 0.20 +1.3 579,585 + GFF3145 0.58 -0.1 579,585 + GFF3145 0.58 +1.6 579,585 + GFF3145 0.58 -0.8 579,866 + -0.3 579,866 + +0.6 579,938 - -1.0 580,210 + GFF3146 0.54 +0.6 580,210 + GFF3146 0.54 -1.0 580,210 + GFF3146 0.54 +0.4 580,211 - GFF3146 0.54 -1.1
Or see this region's nucleotide sequence