Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3133 and GFF3134 are separated by 5 nucleotides GFF3134 and GFF3135 are separated by 88 nucleotides GFF3135 and GFF3136 are separated by 6 nucleotides GFF3136 and GFF3137 are separated by 15 nucleotides
GFF3133 - hypothetical protein, at 563,479 to 564,624
GFF3133
GFF3134 - no description, at 564,630 to 564,902
GFF3134
GFF3135 - no description, at 564,991 to 565,530
GFF3135
GFF3136 - no description, at 565,537 to 566,523
GFF3136
GFF3137 - hypothetical protein, at 566,539 to 568,008
GFF3137
Position (kb)
565
566
567 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 565.054 kb on + strand, within GFF3135 at 565.055 kb on - strand, within GFF3135 at 565.055 kb on - strand, within GFF3135 at 565.078 kb on + strand, within GFF3135 at 565.079 kb on - strand, within GFF3135 at 565.103 kb on - strand, within GFF3135 at 565.286 kb on + strand, within GFF3135 at 565.287 kb on - strand, within GFF3135 at 565.287 kb on - strand, within GFF3135 at 565.318 kb on + strand, within GFF3135 at 565.377 kb on - strand, within GFF3135 at 565.639 kb on + strand, within GFF3136 at 565.650 kb on + strand, within GFF3136 at 565.651 kb on - strand, within GFF3136 at 565.651 kb on - strand, within GFF3136 at 565.718 kb on + strand, within GFF3136 at 565.719 kb on - strand, within GFF3136 at 565.719 kb on - strand, within GFF3136 at 565.775 kb on + strand, within GFF3136 at 565.775 kb on + strand, within GFF3136 at 565.776 kb on - strand, within GFF3136 at 565.776 kb on - strand, within GFF3136 at 565.991 kb on + strand, within GFF3136 at 565.992 kb on - strand, within GFF3136 at 565.992 kb on - strand, within GFF3136 at 566.171 kb on + strand, within GFF3136 at 566.385 kb on - strand, within GFF3136 at 566.526 kb on - strand at 566.526 kb on - strand at 566.526 kb on - strand at 566.540 kb on + strand at 566.887 kb on + strand, within GFF3137 at 566.887 kb on + strand, within GFF3137 at 567.170 kb on + strand, within GFF3137 at 567.171 kb on - strand, within GFF3137 at 567.200 kb on + strand, within GFF3137 at 567.200 kb on + strand, within GFF3137 at 567.200 kb on + strand, within GFF3137 at 567.200 kb on + strand, within GFF3137
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 565,054 + GFF3135 0.12 -0.5 565,055 - GFF3135 0.12 -1.3 565,055 - GFF3135 0.12 -0.8 565,078 + GFF3135 0.16 -1.0 565,079 - GFF3135 0.16 -0.3 565,103 - GFF3135 0.21 -0.7 565,286 + GFF3135 0.55 +0.5 565,287 - GFF3135 0.55 -1.1 565,287 - GFF3135 0.55 -0.9 565,318 + GFF3135 0.61 -1.0 565,377 - GFF3135 0.71 -0.1 565,639 + GFF3136 0.10 -0.9 565,650 + GFF3136 0.11 -1.3 565,651 - GFF3136 0.12 -1.3 565,651 - GFF3136 0.12 -1.7 565,718 + GFF3136 0.18 +0.0 565,719 - GFF3136 0.18 -0.5 565,719 - GFF3136 0.18 -0.2 565,775 + GFF3136 0.24 -0.1 565,775 + GFF3136 0.24 +1.0 565,776 - GFF3136 0.24 -2.3 565,776 - GFF3136 0.24 -0.0 565,991 + GFF3136 0.46 -0.5 565,992 - GFF3136 0.46 +2.0 565,992 - GFF3136 0.46 -1.3 566,171 + GFF3136 0.64 -0.3 566,385 - GFF3136 0.86 -0.8 566,526 - +0.3 566,526 - +0.5 566,526 - -0.1 566,540 + -0.6 566,887 + GFF3137 0.24 -0.5 566,887 + GFF3137 0.24 -0.3 567,170 + GFF3137 0.43 -0.9 567,171 - GFF3137 0.43 -0.8 567,200 + GFF3137 0.45 -0.5 567,200 + GFF3137 0.45 -1.1 567,200 + GFF3137 0.45 -1.9 567,200 + GFF3137 0.45 -0.3
Or see this region's nucleotide sequence