Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2992 and GFF2993 are separated by 8 nucleotides GFF2993 and GFF2994 are separated by 115 nucleotides
GFF2992 - BUG/TctC family periplasmic protein, at 423,116 to 424,105
GFF2992
GFF2993 - Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases, at 424,114 to 425,544
GFF2993
GFF2994 - Transcriptional regulator, LysR family, at 425,660 to 426,610
GFF2994
Position (kb)
424
425
426 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 423.117 kb on + strand at 423.117 kb on + strand at 423.117 kb on + strand at 423.128 kb on - strand at 423.333 kb on + strand, within GFF2992 at 423.334 kb on - strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.537 kb on + strand, within GFF2992 at 423.538 kb on - strand, within GFF2992 at 423.538 kb on - strand, within GFF2992 at 423.538 kb on - strand, within GFF2992 at 423.538 kb on - strand, within GFF2992 at 423.544 kb on + strand, within GFF2992 at 423.544 kb on + strand, within GFF2992 at 423.544 kb on + strand, within GFF2992 at 423.544 kb on + strand, within GFF2992 at 423.544 kb on + strand, within GFF2992 at 423.625 kb on - strand, within GFF2992 at 423.625 kb on - strand, within GFF2992 at 423.798 kb on + strand, within GFF2992 at 423.798 kb on + strand, within GFF2992 at 423.799 kb on - strand, within GFF2992 at 423.799 kb on - strand, within GFF2992 at 423.799 kb on - strand, within GFF2992 at 423.799 kb on - strand, within GFF2992 at 424.277 kb on + strand, within GFF2993 at 424.278 kb on - strand, within GFF2993 at 424.465 kb on + strand, within GFF2993 at 424.465 kb on + strand, within GFF2993 at 424.465 kb on + strand, within GFF2993 at 424.465 kb on + strand, within GFF2993 at 424.465 kb on + strand, within GFF2993 at 424.465 kb on + strand, within GFF2993 at 424.466 kb on - strand, within GFF2993 at 424.466 kb on - strand, within GFF2993 at 425.090 kb on + strand, within GFF2993 at 425.090 kb on + strand, within GFF2993 at 425.091 kb on - strand, within GFF2993 at 425.091 kb on - strand, within GFF2993 at 425.183 kb on + strand, within GFF2993 at 425.184 kb on - strand, within GFF2993 at 425.273 kb on + strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.411 kb on + strand at 425.411 kb on + strand at 425.412 kb on - strand at 425.412 kb on - strand at 425.508 kb on - strand at 425.581 kb on + strand at 425.581 kb on + strand at 425.581 kb on + strand at 425.581 kb on + strand at 425.581 kb on + strand at 425.582 kb on - strand at 425.582 kb on - strand at 425.582 kb on - strand at 425.625 kb on + strand at 425.626 kb on - strand at 425.626 kb on - strand at 425.626 kb on - strand at 425.626 kb on - strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.748 kb on - strand at 426.110 kb on + strand, within GFF2994 at 426.110 kb on + strand, within GFF2994 at 426.110 kb on + strand, within GFF2994 at 426.110 kb on + strand, within GFF2994 at 426.110 kb on + strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.141 kb on + strand, within GFF2994 at 426.142 kb on - strand, within GFF2994 at 426.167 kb on + strand, within GFF2994 at 426.167 kb on + strand, within GFF2994 at 426.167 kb on + strand, within GFF2994 at 426.168 kb on - strand, within GFF2994 at 426.168 kb on - strand, within GFF2994 at 426.168 kb on - strand, within GFF2994 at 426.315 kb on - strand, within GFF2994
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 423,117 + +0.2 423,117 + -1.5 423,117 + +1.6 423,128 - +0.3 423,333 + GFF2992 0.22 +0.2 423,334 - GFF2992 0.22 -0.6 423,537 + GFF2992 0.43 -0.7 423,537 + GFF2992 0.43 +0.9 423,537 + GFF2992 0.43 +0.3 423,537 + GFF2992 0.43 -0.6 423,537 + GFF2992 0.43 -0.2 423,537 + GFF2992 0.43 -0.1 423,537 + GFF2992 0.43 -0.0 423,537 + GFF2992 0.43 +1.0 423,538 - GFF2992 0.43 +0.5 423,538 - GFF2992 0.43 -0.5 423,538 - GFF2992 0.43 -0.7 423,538 - GFF2992 0.43 -1.4 423,544 + GFF2992 0.43 +0.7 423,544 + GFF2992 0.43 +0.1 423,544 + GFF2992 0.43 -0.3 423,544 + GFF2992 0.43 +2.0 423,544 + GFF2992 0.43 +0.3 423,625 - GFF2992 0.51 -1.0 423,625 - GFF2992 0.51 -0.9 423,798 + GFF2992 0.69 +0.5 423,798 + GFF2992 0.69 -1.1 423,799 - GFF2992 0.69 -0.5 423,799 - GFF2992 0.69 +1.7 423,799 - GFF2992 0.69 +0.5 423,799 - GFF2992 0.69 -1.0 424,277 + GFF2993 0.11 +0.5 424,278 - GFF2993 0.11 +1.0 424,465 + GFF2993 0.25 -0.2 424,465 + GFF2993 0.25 +0.0 424,465 + GFF2993 0.25 +0.3 424,465 + GFF2993 0.25 -0.5 424,465 + GFF2993 0.25 +0.4 424,465 + GFF2993 0.25 -0.7 424,466 - GFF2993 0.25 +0.1 424,466 - GFF2993 0.25 +0.5 425,090 + GFF2993 0.68 +0.0 425,090 + GFF2993 0.68 -1.8 425,091 - GFF2993 0.68 -0.8 425,091 - GFF2993 0.68 +0.5 425,183 + GFF2993 0.75 -0.4 425,184 - GFF2993 0.75 +1.0 425,273 + GFF2993 0.81 -0.5 425,274 - GFF2993 0.81 +0.5 425,274 - GFF2993 0.81 +3.3 425,274 - GFF2993 0.81 -1.3 425,274 - GFF2993 0.81 -1.4 425,274 - GFF2993 0.81 +1.2 425,274 - GFF2993 0.81 -0.1 425,274 - GFF2993 0.81 -0.5 425,274 - GFF2993 0.81 +0.4 425,274 - GFF2993 0.81 -0.5 425,274 - GFF2993 0.81 -1.3 425,411 + +0.9 425,411 + -0.5 425,412 - -0.8 425,412 - +0.2 425,508 - +1.5 425,581 + +0.0 425,581 + -0.4 425,581 + +1.5 425,581 + -1.3 425,581 + +1.3 425,582 - +1.0 425,582 - +0.9 425,582 - -0.3 425,625 + -1.1 425,626 - -0.2 425,626 - -0.7 425,626 - -1.0 425,626 - -0.5 425,747 + -1.2 425,747 + +1.0 425,747 + +0.7 425,747 + +0.2 425,747 + -1.0 425,747 + -1.1 425,747 + +0.4 425,747 + -0.7 425,747 + -2.5 425,747 + -0.2 425,748 - -0.3 426,110 + GFF2994 0.47 +0.6 426,110 + GFF2994 0.47 +0.5 426,110 + GFF2994 0.47 -0.6 426,110 + GFF2994 0.47 +0.2 426,110 + GFF2994 0.47 -1.1 426,111 - GFF2994 0.47 +0.8 426,111 - GFF2994 0.47 -1.1 426,111 - GFF2994 0.47 -1.3 426,111 - GFF2994 0.47 -0.2 426,111 - GFF2994 0.47 +1.0 426,111 - GFF2994 0.47 -1.8 426,141 + GFF2994 0.51 +0.3 426,142 - GFF2994 0.51 -0.3 426,167 + GFF2994 0.53 +1.6 426,167 + GFF2994 0.53 +0.1 426,167 + GFF2994 0.53 -0.0 426,168 - GFF2994 0.53 +0.5 426,168 - GFF2994 0.53 -1.2 426,168 - GFF2994 0.53 -0.0 426,315 - GFF2994 0.69 -1.3
Or see this region's nucleotide sequence