Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2594 and GFF2595 are separated by 133 nucleotides GFF2595 and GFF2596 overlap by 4 nucleotides
GFF2594 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 8 to 1,036
GFF2594
GFF2595 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 1,170 to 1,934
GFF2595
GFF2596 - Multidrug resistance transporter, Bcr/CflA family, at 1,931 to 3,151
GFF2596
Position (kb) 0
1
2 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 0.060 kb on + strand at 0.076 kb on + strand at 0.250 kb on - strand, within GFF2594 at 0.343 kb on - strand, within GFF2594 at 0.603 kb on + strand, within GFF2594 at 0.603 kb on + strand, within GFF2594 at 0.603 kb on + strand, within GFF2594 at 0.603 kb on + strand, within GFF2594 at 0.604 kb on - strand, within GFF2594 at 0.613 kb on + strand, within GFF2594 at 0.613 kb on + strand, within GFF2594 at 0.613 kb on + strand, within GFF2594 at 0.613 kb on + strand, within GFF2594 at 0.613 kb on + strand, within GFF2594 at 0.613 kb on + strand, within GFF2594 at 0.613 kb on + strand, within GFF2594 at 0.613 kb on + strand, within GFF2594 at 0.613 kb on + strand, within GFF2594 at 0.613 kb on + strand, within GFF2594 at 0.614 kb on - strand, within GFF2594 at 0.614 kb on - strand, within GFF2594 at 0.614 kb on - strand, within GFF2594 at 0.614 kb on - strand, within GFF2594 at 0.614 kb on - strand, within GFF2594 at 0.614 kb on - strand, within GFF2594 at 0.614 kb on - strand, within GFF2594 at 0.614 kb on - strand, within GFF2594 at 0.614 kb on - strand, within GFF2594 at 0.614 kb on - strand, within GFF2594 at 0.614 kb on - strand, within GFF2594 at 0.614 kb on - strand, within GFF2594 at 0.858 kb on + strand, within GFF2594 at 0.858 kb on + strand, within GFF2594 at 0.859 kb on - strand, within GFF2594 at 1.048 kb on + strand at 1.048 kb on + strand at 1.048 kb on + strand at 1.075 kb on + strand at 1.075 kb on + strand at 1.088 kb on - strand at 1.088 kb on - strand at 1.139 kb on - strand at 1.139 kb on - strand at 1.144 kb on + strand at 1.145 kb on - strand at 1.411 kb on + strand, within GFF2595 at 1.411 kb on + strand, within GFF2595 at 1.411 kb on + strand, within GFF2595 at 1.411 kb on + strand, within GFF2595 at 1.411 kb on + strand, within GFF2595 at 1.411 kb on + strand, within GFF2595 at 1.411 kb on + strand, within GFF2595 at 1.412 kb on - strand, within GFF2595 at 1.412 kb on - strand, within GFF2595 at 1.412 kb on - strand, within GFF2595 at 1.412 kb on - strand, within GFF2595 at 1.588 kb on + strand, within GFF2595 at 1.588 kb on + strand, within GFF2595 at 1.805 kb on - strand, within GFF2595
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 60 + +0.4 76 + +0.1 250 - GFF2594 0.24 -0.8 343 - GFF2594 0.33 +0.8 603 + GFF2594 0.58 -0.7 603 + GFF2594 0.58 -1.6 603 + GFF2594 0.58 -0.3 603 + GFF2594 0.58 -1.3 604 - GFF2594 0.58 -0.3 613 + GFF2594 0.59 +0.0 613 + GFF2594 0.59 +0.5 613 + GFF2594 0.59 -0.7 613 + GFF2594 0.59 +0.2 613 + GFF2594 0.59 +1.0 613 + GFF2594 0.59 -0.7 613 + GFF2594 0.59 -0.7 613 + GFF2594 0.59 -0.8 613 + GFF2594 0.59 +0.5 613 + GFF2594 0.59 +1.0 614 - GFF2594 0.59 -0.5 614 - GFF2594 0.59 -0.8 614 - GFF2594 0.59 +3.0 614 - GFF2594 0.59 -1.7 614 - GFF2594 0.59 -0.6 614 - GFF2594 0.59 -0.0 614 - GFF2594 0.59 -0.8 614 - GFF2594 0.59 +1.4 614 - GFF2594 0.59 +0.3 614 - GFF2594 0.59 +0.4 614 - GFF2594 0.59 -0.1 614 - GFF2594 0.59 -1.3 858 + GFF2594 0.83 -1.0 858 + GFF2594 0.83 -0.7 859 - GFF2594 0.83 +0.0 1,048 + +0.5 1,048 + -0.5 1,048 + +1.6 1,075 + -0.5 1,075 + +0.0 1,088 - -2.0 1,088 - +1.6 1,139 - +0.3 1,139 - +0.4 1,144 + +0.4 1,145 - +0.4 1,411 + GFF2595 0.32 -0.3 1,411 + GFF2595 0.32 -0.3 1,411 + GFF2595 0.32 -0.5 1,411 + GFF2595 0.32 -0.3 1,411 + GFF2595 0.32 -0.4 1,411 + GFF2595 0.32 -1.7 1,411 + GFF2595 0.32 -0.4 1,412 - GFF2595 0.32 +0.4 1,412 - GFF2595 0.32 -1.1 1,412 - GFF2595 0.32 +0.4 1,412 - GFF2595 0.32 -0.1 1,588 + GFF2595 0.55 +0.5 1,588 + GFF2595 0.55 -0.4 1,805 - GFF2595 0.83 +0.8
Or see this region's nucleotide sequence