Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2334 and GFF2335 are separated by 79 nucleotides GFF2335 and GFF2336 overlap by 4 nucleotides GFF2336 and GFF2337 are separated by 141 nucleotides
GFF2334 - Beta-lactamase class C-like and penicillin binding proteins (PBPs) superfamily, at 384,173 to 385,363
GFF2334
GFF2335 - Lactoylglutathione lyase and related lyases, at 385,443 to 385,859
GFF2335
GFF2336 - Transcriptional regulator, AraC family, at 385,856 to 386,329
GFF2336
GFF2337 - hypothetical protein, at 386,471 to 388,066
GFF2337
Position (kb) 385
386 Strain fitness (log2 ratio) -2
-1
0
1 at 384.651 kb on + strand, within GFF2334 at 384.651 kb on + strand, within GFF2334 at 384.652 kb on - strand, within GFF2334 at 384.673 kb on - strand, within GFF2334 at 384.673 kb on - strand, within GFF2334 at 384.724 kb on - strand, within GFF2334 at 384.724 kb on - strand, within GFF2334 at 384.777 kb on + strand, within GFF2334 at 384.777 kb on + strand, within GFF2334 at 384.778 kb on - strand, within GFF2334 at 384.786 kb on + strand, within GFF2334 at 384.786 kb on + strand, within GFF2334 at 384.786 kb on + strand, within GFF2334 at 385.182 kb on + strand, within GFF2334 at 385.183 kb on - strand, within GFF2334 at 385.183 kb on - strand, within GFF2334 at 385.183 kb on - strand, within GFF2334 at 385.183 kb on - strand, within GFF2334 at 385.193 kb on + strand, within GFF2334 at 385.292 kb on + strand at 385.357 kb on + strand at 385.358 kb on - strand at 385.358 kb on - strand at 385.358 kb on - strand at 385.576 kb on + strand, within GFF2335 at 385.576 kb on + strand, within GFF2335 at 385.577 kb on - strand, within GFF2335 at 385.577 kb on - strand, within GFF2335 at 385.745 kb on - strand, within GFF2335 at 385.795 kb on + strand, within GFF2335 at 385.795 kb on + strand, within GFF2335 at 385.795 kb on + strand, within GFF2335 at 385.795 kb on + strand, within GFF2335 at 385.795 kb on + strand, within GFF2335 at 385.795 kb on + strand, within GFF2335 at 385.795 kb on + strand, within GFF2335 at 385.796 kb on - strand, within GFF2335 at 385.796 kb on - strand, within GFF2335 at 385.796 kb on - strand, within GFF2335 at 385.828 kb on + strand at 385.829 kb on - strand at 385.829 kb on - strand at 385.829 kb on - strand at 385.829 kb on - strand at 385.829 kb on - strand at 385.829 kb on - strand at 385.829 kb on - strand at 385.836 kb on + strand at 385.837 kb on - strand at 385.837 kb on - strand at 385.847 kb on + strand at 385.847 kb on + strand at 386.163 kb on + strand, within GFF2336 at 386.231 kb on + strand, within GFF2336 at 386.424 kb on + strand at 386.424 kb on + strand at 386.425 kb on - strand at 386.425 kb on - strand at 386.425 kb on - strand at 386.493 kb on + strand at 386.493 kb on + strand at 386.494 kb on - strand at 386.495 kb on + strand at 386.495 kb on + strand at 386.496 kb on - strand at 386.496 kb on - strand at 386.496 kb on - strand at 386.496 kb on - strand at 386.709 kb on + strand, within GFF2337 at 386.710 kb on - strand, within GFF2337 at 386.778 kb on + strand, within GFF2337 at 386.823 kb on + strand, within GFF2337 at 386.823 kb on + strand, within GFF2337 at 386.824 kb on - strand, within GFF2337 at 386.824 kb on - strand, within GFF2337 at 386.829 kb on + strand, within GFF2337 at 386.830 kb on - strand, within GFF2337
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 384,651 + GFF2334 0.40 +1.3 384,651 + GFF2334 0.40 -1.0 384,652 - GFF2334 0.40 +0.2 384,673 - GFF2334 0.42 -0.0 384,673 - GFF2334 0.42 -0.1 384,724 - GFF2334 0.46 +0.2 384,724 - GFF2334 0.46 -0.2 384,777 + GFF2334 0.51 -1.3 384,777 + GFF2334 0.51 -0.3 384,778 - GFF2334 0.51 +0.1 384,786 + GFF2334 0.51 -0.1 384,786 + GFF2334 0.51 -0.9 384,786 + GFF2334 0.51 -0.1 385,182 + GFF2334 0.85 -0.3 385,183 - GFF2334 0.85 -1.7 385,183 - GFF2334 0.85 -0.9 385,183 - GFF2334 0.85 +0.0 385,183 - GFF2334 0.85 +0.1 385,193 + GFF2334 0.86 -0.5 385,292 + +1.2 385,357 + +0.1 385,358 - +0.5 385,358 - +0.3 385,358 - -1.9 385,576 + GFF2335 0.32 +0.8 385,576 + GFF2335 0.32 -0.3 385,577 - GFF2335 0.32 -1.0 385,577 - GFF2335 0.32 +0.1 385,745 - GFF2335 0.72 -0.4 385,795 + GFF2335 0.84 +0.1 385,795 + GFF2335 0.84 -0.4 385,795 + GFF2335 0.84 -0.3 385,795 + GFF2335 0.84 -0.5 385,795 + GFF2335 0.84 +0.3 385,795 + GFF2335 0.84 -0.1 385,795 + GFF2335 0.84 -1.0 385,796 - GFF2335 0.85 -0.3 385,796 - GFF2335 0.85 -0.9 385,796 - GFF2335 0.85 -0.1 385,828 + -0.3 385,829 - -2.3 385,829 - -0.8 385,829 - +0.3 385,829 - -1.3 385,829 - -1.4 385,829 - +0.1 385,829 - +1.2 385,836 + -1.8 385,837 - -0.3 385,837 - -0.1 385,847 + -0.8 385,847 + +1.2 386,163 + GFF2336 0.65 +0.1 386,231 + GFF2336 0.79 -0.5 386,424 + -0.2 386,424 + +0.8 386,425 - -0.1 386,425 - +1.0 386,425 - -0.6 386,493 + +0.3 386,493 + -1.2 386,494 - -0.7 386,495 + -0.4 386,495 + +0.2 386,496 - +0.2 386,496 - +1.1 386,496 - -0.5 386,496 - -0.9 386,709 + GFF2337 0.15 -1.0 386,710 - GFF2337 0.15 -1.3 386,778 + GFF2337 0.19 -0.4 386,823 + GFF2337 0.22 -0.3 386,823 + GFF2337 0.22 +0.6 386,824 - GFF2337 0.22 +0.5 386,824 - GFF2337 0.22 +0.8 386,829 + GFF2337 0.22 +0.2 386,830 - GFF2337 0.22 -0.7
Or see this region's nucleotide sequence