Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2055 and GFF2056 overlap by 4 nucleotides GFF2056 and GFF2057 are separated by 30 nucleotides GFF2057 and GFF2058 are separated by 84 nucleotides GFF2058 and GFF2059 overlap by 4 nucleotides
GFF2055 - 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20), at 104,051 to 104,875
GFF2055
GFF2056 - Septum-associated rare lipoprotein A, at 104,872 to 105,582
GFF2056
GFF2057 - 23S rRNA (adenine(2030)-N(6))-methyltransferase (EC 2.1.1.266), at 105,613 to 106,578
GFF2057
GFF2058 - hypothetical protein, at 106,663 to 107,379
GFF2058
GFF2059 - Putative serine protease, at 107,376 to 108,656
GFF2059
Position (kb)
105
106
107 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 104.822 kb on + strand at 104.822 kb on + strand at 104.823 kb on - strand at 104.823 kb on - strand at 104.823 kb on - strand at 104.823 kb on - strand at 104.823 kb on - strand at 104.823 kb on - strand at 104.823 kb on - strand at 104.823 kb on - strand at 105.080 kb on - strand, within GFF2056 at 105.178 kb on + strand, within GFF2056 at 105.179 kb on - strand, within GFF2056 at 105.179 kb on - strand, within GFF2056 at 105.341 kb on - strand, within GFF2056 at 105.341 kb on - strand, within GFF2056 at 105.580 kb on + strand at 105.580 kb on + strand at 105.580 kb on + strand at 105.580 kb on + strand at 105.581 kb on - strand at 105.581 kb on - strand at 105.581 kb on - strand at 105.710 kb on + strand, within GFF2057 at 105.710 kb on + strand, within GFF2057 at 105.710 kb on + strand, within GFF2057 at 105.711 kb on - strand, within GFF2057 at 105.711 kb on - strand, within GFF2057 at 105.914 kb on + strand, within GFF2057 at 105.914 kb on + strand, within GFF2057 at 105.914 kb on + strand, within GFF2057 at 105.914 kb on + strand, within GFF2057 at 105.914 kb on + strand, within GFF2057 at 105.915 kb on - strand, within GFF2057 at 105.915 kb on - strand, within GFF2057 at 105.915 kb on - strand, within GFF2057 at 105.915 kb on - strand, within GFF2057 at 105.915 kb on - strand, within GFF2057 at 105.915 kb on - strand, within GFF2057 at 105.915 kb on - strand, within GFF2057 at 105.971 kb on + strand, within GFF2057 at 105.972 kb on - strand, within GFF2057 at 105.989 kb on + strand, within GFF2057 at 105.989 kb on + strand, within GFF2057 at 105.989 kb on + strand, within GFF2057 at 105.989 kb on + strand, within GFF2057 at 105.990 kb on - strand, within GFF2057 at 105.990 kb on - strand, within GFF2057 at 105.990 kb on - strand, within GFF2057 at 105.990 kb on - strand, within GFF2057 at 105.990 kb on - strand, within GFF2057 at 106.208 kb on + strand, within GFF2057 at 106.208 kb on + strand, within GFF2057 at 106.256 kb on + strand, within GFF2057 at 106.418 kb on + strand, within GFF2057 at 106.418 kb on + strand, within GFF2057 at 106.418 kb on + strand, within GFF2057 at 106.418 kb on + strand, within GFF2057 at 106.418 kb on + strand, within GFF2057 at 106.419 kb on - strand, within GFF2057 at 106.419 kb on - strand, within GFF2057 at 106.419 kb on - strand, within GFF2057 at 106.419 kb on - strand, within GFF2057 at 106.419 kb on - strand, within GFF2057 at 106.419 kb on - strand, within GFF2057 at 106.437 kb on - strand, within GFF2057 at 106.437 kb on - strand, within GFF2057 at 106.568 kb on + strand at 106.569 kb on - strand at 106.569 kb on - strand at 106.614 kb on + strand at 106.614 kb on + strand at 106.614 kb on + strand at 106.614 kb on + strand at 106.614 kb on + strand at 106.614 kb on + strand at 106.614 kb on + strand at 106.614 kb on + strand at 106.614 kb on + strand at 106.614 kb on + strand at 106.614 kb on + strand at 106.615 kb on - strand at 106.615 kb on - strand at 106.615 kb on - strand at 106.615 kb on - strand at 106.615 kb on - strand at 106.615 kb on - strand at 106.615 kb on - strand at 106.615 kb on - strand at 106.615 kb on - strand at 106.615 kb on - strand at 106.615 kb on - strand at 106.615 kb on - strand at 106.615 kb on - strand at 106.641 kb on - strand at 106.719 kb on + strand at 106.719 kb on + strand at 106.719 kb on + strand at 106.720 kb on - strand at 106.720 kb on - strand at 107.032 kb on + strand, within GFF2058 at 107.032 kb on + strand, within GFF2058 at 107.032 kb on + strand, within GFF2058 at 107.032 kb on + strand, within GFF2058 at 107.032 kb on + strand, within GFF2058 at 107.032 kb on + strand, within GFF2058 at 107.033 kb on - strand, within GFF2058 at 107.038 kb on + strand, within GFF2058 at 107.038 kb on + strand, within GFF2058 at 107.038 kb on + strand, within GFF2058 at 107.038 kb on + strand, within GFF2058 at 107.038 kb on + strand, within GFF2058 at 107.038 kb on + strand, within GFF2058 at 107.038 kb on + strand, within GFF2058 at 107.038 kb on + strand, within GFF2058 at 107.038 kb on + strand, within GFF2058 at 107.038 kb on + strand, within GFF2058 at 107.039 kb on - strand, within GFF2058 at 107.039 kb on - strand, within GFF2058 at 107.039 kb on - strand, within GFF2058 at 107.039 kb on - strand, within GFF2058 at 107.039 kb on - strand, within GFF2058 at 107.039 kb on - strand, within GFF2058 at 107.039 kb on - strand, within GFF2058 at 107.039 kb on - strand, within GFF2058 at 107.039 kb on - strand, within GFF2058 at 107.039 kb on - strand, within GFF2058
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 104,822 + +0.2 104,822 + -0.5 104,823 - +0.4 104,823 - -1.6 104,823 - -0.6 104,823 - -0.7 104,823 - +0.9 104,823 - -0.4 104,823 - -0.6 104,823 - -0.9 105,080 - GFF2056 0.29 -1.0 105,178 + GFF2056 0.43 +0.1 105,179 - GFF2056 0.43 -0.9 105,179 - GFF2056 0.43 +0.3 105,341 - GFF2056 0.66 +0.9 105,341 - GFF2056 0.66 +1.0 105,580 + +0.9 105,580 + -2.3 105,580 + -1.2 105,580 + -0.3 105,581 - -0.1 105,581 - -0.4 105,581 - +0.6 105,710 + GFF2057 0.10 -1.6 105,710 + GFF2057 0.10 +0.2 105,710 + GFF2057 0.10 +1.9 105,711 - GFF2057 0.10 +1.3 105,711 - GFF2057 0.10 -1.6 105,914 + GFF2057 0.31 -1.0 105,914 + GFF2057 0.31 +0.2 105,914 + GFF2057 0.31 +0.0 105,914 + GFF2057 0.31 -0.4 105,914 + GFF2057 0.31 -0.4 105,915 - GFF2057 0.31 -0.0 105,915 - GFF2057 0.31 -1.2 105,915 - GFF2057 0.31 -0.6 105,915 - GFF2057 0.31 -0.7 105,915 - GFF2057 0.31 -0.1 105,915 - GFF2057 0.31 -0.4 105,915 - GFF2057 0.31 -0.7 105,971 + GFF2057 0.37 +0.3 105,972 - GFF2057 0.37 -0.6 105,989 + GFF2057 0.39 +0.2 105,989 + GFF2057 0.39 -2.6 105,989 + GFF2057 0.39 +0.4 105,989 + GFF2057 0.39 -2.1 105,990 - GFF2057 0.39 -1.9 105,990 - GFF2057 0.39 -1.9 105,990 - GFF2057 0.39 -1.6 105,990 - GFF2057 0.39 -0.8 105,990 - GFF2057 0.39 -0.4 106,208 + GFF2057 0.62 -0.5 106,208 + GFF2057 0.62 -0.1 106,256 + GFF2057 0.67 -0.2 106,418 + GFF2057 0.83 +1.5 106,418 + GFF2057 0.83 -0.7 106,418 + GFF2057 0.83 +1.4 106,418 + GFF2057 0.83 +0.4 106,418 + GFF2057 0.83 -1.7 106,419 - GFF2057 0.83 -1.4 106,419 - GFF2057 0.83 +0.6 106,419 - GFF2057 0.83 -0.2 106,419 - GFF2057 0.83 -0.5 106,419 - GFF2057 0.83 -0.7 106,419 - GFF2057 0.83 -0.7 106,437 - GFF2057 0.85 -0.5 106,437 - GFF2057 0.85 -0.1 106,568 + -1.6 106,569 - -0.2 106,569 - -0.6 106,614 + -0.7 106,614 + +0.6 106,614 + -1.7 106,614 + -0.0 106,614 + +0.3 106,614 + -0.3 106,614 + +1.8 106,614 + -0.3 106,614 + -0.7 106,614 + +1.0 106,614 + +0.2 106,615 - -1.4 106,615 - -0.6 106,615 - +1.5 106,615 - -0.1 106,615 - -0.2 106,615 - -0.2 106,615 - +0.4 106,615 - +0.4 106,615 - -1.2 106,615 - -1.0 106,615 - -0.2 106,615 - +0.7 106,615 - -0.1 106,641 - -0.5 106,719 + -0.1 106,719 + +0.1 106,719 + -0.7 106,720 - -0.4 106,720 - -0.5 107,032 + GFF2058 0.51 -0.6 107,032 + GFF2058 0.51 -0.1 107,032 + GFF2058 0.51 +1.2 107,032 + GFF2058 0.51 +1.5 107,032 + GFF2058 0.51 -0.8 107,032 + GFF2058 0.51 -0.6 107,033 - GFF2058 0.52 -0.2 107,038 + GFF2058 0.52 -0.2 107,038 + GFF2058 0.52 +0.1 107,038 + GFF2058 0.52 +0.0 107,038 + GFF2058 0.52 -0.2 107,038 + GFF2058 0.52 -0.7 107,038 + GFF2058 0.52 -1.2 107,038 + GFF2058 0.52 -0.4 107,038 + GFF2058 0.52 +0.6 107,038 + GFF2058 0.52 +1.5 107,038 + GFF2058 0.52 -0.2 107,039 - GFF2058 0.52 -0.8 107,039 - GFF2058 0.52 -0.9 107,039 - GFF2058 0.52 -0.9 107,039 - GFF2058 0.52 -0.4 107,039 - GFF2058 0.52 -0.5 107,039 - GFF2058 0.52 -0.6 107,039 - GFF2058 0.52 +0.5 107,039 - GFF2058 0.52 +0.9 107,039 - GFF2058 0.52 +0.6 107,039 - GFF2058 0.52 -0.2
Or see this region's nucleotide sequence