Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1672 and GFF1673 are separated by 30 nucleotides GFF1673 and GFF1674 overlap by 4 nucleotides GFF1674 and GFF1675 overlap by 8 nucleotides GFF1675 and GFF1676 overlap by 4 nucleotides
GFF1672 - BUG/TctC family periplasmic protein, at 317,860 to 318,825
GFF1672
GFF1673 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 318,856 to 319,566
GFF1673
GFF1674 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 319,563 to 320,324
GFF1674
GFF1675 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 320,317 to 321,297
GFF1675
GFF1676 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 321,294 to 322,166
GFF1676
Position (kb) 319
320
321 Strain fitness (log2 ratio) -2
-1
0
1 at 318.947 kb on + strand, within GFF1673 at 318.947 kb on + strand, within GFF1673 at 318.947 kb on + strand, within GFF1673 at 319.203 kb on + strand, within GFF1673 at 319.621 kb on + strand at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.597 kb on - strand, within GFF1675 at 320.601 kb on - strand, within GFF1675 at 320.601 kb on - strand, within GFF1675 at 320.618 kb on + strand, within GFF1675 at 320.618 kb on + strand, within GFF1675 at 320.618 kb on + strand, within GFF1675 at 320.619 kb on - strand, within GFF1675 at 320.619 kb on - strand, within GFF1675 at 321.089 kb on + strand, within GFF1675 at 321.090 kb on - strand, within GFF1675 at 321.090 kb on - strand, within GFF1675 at 321.090 kb on - strand, within GFF1675 at 321.182 kb on + strand, within GFF1675 at 321.183 kb on - strand, within GFF1675 at 321.183 kb on - strand, within GFF1675
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 318,947 + GFF1673 0.13 -1.3 318,947 + GFF1673 0.13 +0.4 318,947 + GFF1673 0.13 -0.4 319,203 + GFF1673 0.49 -1.5 319,621 + -0.4 320,423 + GFF1675 0.11 -0.2 320,423 + GFF1675 0.11 -0.5 320,423 + GFF1675 0.11 -0.3 320,423 + GFF1675 0.11 -0.2 320,423 + GFF1675 0.11 -1.9 320,597 - GFF1675 0.29 +0.1 320,601 - GFF1675 0.29 -0.9 320,601 - GFF1675 0.29 -0.4 320,618 + GFF1675 0.31 -0.7 320,618 + GFF1675 0.31 -0.7 320,618 + GFF1675 0.31 +1.6 320,619 - GFF1675 0.31 -0.4 320,619 - GFF1675 0.31 +0.2 321,089 + GFF1675 0.79 -0.3 321,090 - GFF1675 0.79 +0.2 321,090 - GFF1675 0.79 +0.2 321,090 - GFF1675 0.79 -1.4 321,182 + GFF1675 0.88 -0.4 321,183 - GFF1675 0.88 +0.6 321,183 - GFF1675 0.88 -1.5
Or see this region's nucleotide sequence