Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1641 and GFF1642 are separated by 63 nucleotides GFF1642 and GFF1643 are separated by 4 nucleotides GFF1643 and GFF1644 are separated by 205 nucleotides GFF1644 and GFF1645 are separated by 25 nucleotides
GFF1641 - Polyketide synthase modules and related proteins, at 278,275 to 283,587
GFF1641
GFF1642 - FIG057993:Thioesterase involved in non-ribosomal peptide biosynthesis, at 283,651 to 284,391
GFF1642
GFF1643 - MbtH-like NRPS chaperone, at 284,396 to 284,644
GFF1643
GFF1644 - hypothetical protein, at 284,850 to 285,110
GFF1644
GFF1645 - Sigma factor PvdS, controling pyoverdin biosynthesis, at 285,136 to 285,666
GFF1645
Position (kb)
284
285 Strain fitness (log2 ratio)
-2
-1
0
1 at 284.333 kb on + strand at 284.333 kb on + strand at 284.333 kb on + strand at 284.333 kb on + strand at 284.333 kb on + strand at 284.333 kb on + strand at 284.333 kb on + strand at 284.334 kb on - strand at 284.334 kb on - strand at 284.334 kb on - strand at 284.334 kb on - strand at 284.334 kb on - strand at 284.334 kb on - strand at 284.334 kb on - strand at 284.486 kb on + strand, within GFF1643 at 284.486 kb on + strand, within GFF1643 at 284.486 kb on + strand, within GFF1643 at 284.486 kb on + strand, within GFF1643 at 284.486 kb on + strand, within GFF1643 at 284.487 kb on - strand, within GFF1643 at 284.487 kb on - strand, within GFF1643 at 284.490 kb on + strand, within GFF1643 at 284.490 kb on + strand, within GFF1643 at 284.491 kb on - strand, within GFF1643 at 284.491 kb on - strand, within GFF1643 at 284.577 kb on + strand, within GFF1643 at 284.577 kb on + strand, within GFF1643 at 284.577 kb on + strand, within GFF1643 at 284.638 kb on + strand at 284.638 kb on + strand at 284.638 kb on + strand at 284.714 kb on + strand at 284.715 kb on - strand at 284.742 kb on + strand at 284.742 kb on + strand at 284.742 kb on + strand at 284.951 kb on - strand, within GFF1644 at 285.404 kb on + strand, within GFF1645 at 285.404 kb on + strand, within GFF1645 at 285.404 kb on + strand, within GFF1645 at 285.404 kb on + strand, within GFF1645 at 285.405 kb on - strand, within GFF1645 at 285.405 kb on - strand, within GFF1645 at 285.443 kb on + strand, within GFF1645 at 285.479 kb on + strand, within GFF1645 at 285.479 kb on + strand, within GFF1645 at 285.480 kb on - strand, within GFF1645 at 285.533 kb on + strand, within GFF1645
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 284,333 + -1.1 284,333 + +1.5 284,333 + -0.1 284,333 + -0.1 284,333 + -0.2 284,333 + -0.7 284,333 + +0.3 284,334 - -0.1 284,334 - +0.5 284,334 - -0.7 284,334 - +0.1 284,334 - +0.2 284,334 - -0.4 284,334 - +0.3 284,486 + GFF1643 0.36 -1.4 284,486 + GFF1643 0.36 -2.4 284,486 + GFF1643 0.36 -0.7 284,486 + GFF1643 0.36 +0.6 284,486 + GFF1643 0.36 -0.8 284,487 - GFF1643 0.37 -1.5 284,487 - GFF1643 0.37 -0.6 284,490 + GFF1643 0.38 -0.0 284,490 + GFF1643 0.38 -0.0 284,491 - GFF1643 0.38 -0.1 284,491 - GFF1643 0.38 -0.7 284,577 + GFF1643 0.73 -0.5 284,577 + GFF1643 0.73 +0.4 284,577 + GFF1643 0.73 -0.5 284,638 + +0.1 284,638 + -0.1 284,638 + +0.2 284,714 + -0.3 284,715 - -2.1 284,742 + -0.6 284,742 + -0.5 284,742 + -0.6 284,951 - GFF1644 0.39 +0.0 285,404 + GFF1645 0.50 -0.0 285,404 + GFF1645 0.50 -0.0 285,404 + GFF1645 0.50 -0.3 285,404 + GFF1645 0.50 -0.1 285,405 - GFF1645 0.51 -1.7 285,405 - GFF1645 0.51 +0.1 285,443 + GFF1645 0.58 +0.1 285,479 + GFF1645 0.65 +0.6 285,479 + GFF1645 0.65 +0.2 285,480 - GFF1645 0.65 +0.0 285,533 + GFF1645 0.75 +1.2
Or see this region's nucleotide sequence