Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1621 and GFF1622 are separated by 23 nucleotides GFF1622 and GFF1623 are separated by 59 nucleotides
GFF1621 - Phosphonoacetaldehyde dehydrogenase (NAD+), at 220,563 to 222,011
GFF1621
GFF1622 - Phosphonoacetate hydrolase (EC 3.11.1.2), at 222,035 to 223,261
GFF1622
GFF1623 - Glycerol-3-phosphate transporter, at 223,321 to 224,631
GFF1623
Position (kb) 222
223
224 Strain fitness (log2 ratio) -2
-1
0
1
2 at 221.164 kb on + strand, within GFF1621 at 221.164 kb on + strand, within GFF1621 at 221.263 kb on + strand, within GFF1621 at 221.585 kb on - strand, within GFF1621 at 221.914 kb on - strand at 223.003 kb on - strand, within GFF1622 at 223.292 kb on - strand at 223.454 kb on + strand, within GFF1623 at 223.454 kb on + strand, within GFF1623 at 223.455 kb on - strand, within GFF1623 at 223.607 kb on + strand, within GFF1623 at 223.607 kb on + strand, within GFF1623 at 223.607 kb on + strand, within GFF1623 at 223.607 kb on + strand, within GFF1623 at 223.607 kb on + strand, within GFF1623 at 223.607 kb on + strand, within GFF1623 at 223.608 kb on - strand, within GFF1623 at 223.608 kb on - strand, within GFF1623 at 223.608 kb on - strand, within GFF1623 at 223.608 kb on - strand, within GFF1623 at 223.608 kb on - strand, within GFF1623 at 223.643 kb on + strand, within GFF1623 at 223.644 kb on - strand, within GFF1623 at 223.661 kb on + strand, within GFF1623 at 223.902 kb on - strand, within GFF1623 at 224.030 kb on + strand, within GFF1623 at 224.031 kb on - strand, within GFF1623 at 224.165 kb on + strand, within GFF1623 at 224.166 kb on - strand, within GFF1623 at 224.166 kb on - strand, within GFF1623 at 224.253 kb on - strand, within GFF1623
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 221,164 + GFF1621 0.41 +0.5 221,164 + GFF1621 0.41 -0.7 221,263 + GFF1621 0.48 -0.1 221,585 - GFF1621 0.71 -0.4 221,914 - +1.6 223,003 - GFF1622 0.79 +0.9 223,292 - -1.3 223,454 + GFF1623 0.10 -0.4 223,454 + GFF1623 0.10 -1.9 223,455 - GFF1623 0.10 +0.1 223,607 + GFF1623 0.22 -0.9 223,607 + GFF1623 0.22 -0.8 223,607 + GFF1623 0.22 +0.9 223,607 + GFF1623 0.22 -0.1 223,607 + GFF1623 0.22 -0.3 223,607 + GFF1623 0.22 -0.1 223,608 - GFF1623 0.22 +2.0 223,608 - GFF1623 0.22 -0.3 223,608 - GFF1623 0.22 -1.0 223,608 - GFF1623 0.22 -0.0 223,608 - GFF1623 0.22 -0.6 223,643 + GFF1623 0.25 +0.8 223,644 - GFF1623 0.25 -0.8 223,661 + GFF1623 0.26 +0.3 223,902 - GFF1623 0.44 -0.1 224,030 + GFF1623 0.54 +0.0 224,031 - GFF1623 0.54 +0.6 224,165 + GFF1623 0.64 -0.3 224,166 - GFF1623 0.64 -1.5 224,166 - GFF1623 0.64 +0.0 224,253 - GFF1623 0.71 -0.6
Or see this region's nucleotide sequence