Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1562 and GFF1563 are separated by 165 nucleotides GFF1563 and GFF1564 are separated by 11 nucleotides GFF1564 and GFF1565 are separated by 276 nucleotides GFF1565 and GFF1566 are separated by 551 nucleotides GFF1566 and GFF1567 are separated by 63 nucleotides
GFF1562 - Transcriptional regulator, LysR family, at 163,997 to 164,920
GFF1562
GFF1563 - no description, at 165,086 to 165,319
GFF1563
GFF1564 - Prevent host death protein, Phd antitoxin, at 165,331 to 165,465
GFF1564
GFF1565 - hypothetical protein, at 165,742 to 166,905
GFF1565
GFF1566 - no description, at 167,457 to 167,657
GFF1566
GFF1567 - Demethylmenaquinone methyltransferase-like protein, at 167,721 to 168,446
GFF1567
Position (kb) 165
166
167 Strain fitness (log2 ratio) -2
-1
0
1
2 at 164.816 kb on + strand, within GFF1562 at 164.816 kb on + strand, within GFF1562 at 164.817 kb on - strand, within GFF1562 at 164.817 kb on - strand, within GFF1562 at 164.817 kb on - strand, within GFF1562 at 164.817 kb on - strand, within GFF1562 at 164.919 kb on - strand at 165.024 kb on + strand at 165.024 kb on + strand at 165.024 kb on + strand at 165.024 kb on + strand at 165.025 kb on - strand at 165.025 kb on - strand at 165.025 kb on - strand at 165.025 kb on - strand at 165.025 kb on - strand at 165.028 kb on - strand at 165.056 kb on + strand at 165.057 kb on - strand at 165.057 kb on - strand at 165.059 kb on + strand at 165.059 kb on + strand at 165.059 kb on + strand at 165.060 kb on - strand at 165.088 kb on - strand at 165.093 kb on + strand at 165.093 kb on + strand at 165.093 kb on + strand at 165.093 kb on + strand at 165.094 kb on - strand at 165.094 kb on - strand at 165.106 kb on - strand at 165.124 kb on - strand, within GFF1563 at 165.173 kb on + strand, within GFF1563 at 165.173 kb on + strand, within GFF1563 at 165.212 kb on + strand, within GFF1563 at 165.213 kb on - strand, within GFF1563 at 165.213 kb on - strand, within GFF1563 at 165.213 kb on - strand, within GFF1563 at 165.213 kb on - strand, within GFF1563 at 165.213 kb on - strand, within GFF1563 at 165.278 kb on + strand, within GFF1563 at 165.278 kb on + strand, within GFF1563 at 165.278 kb on + strand, within GFF1563 at 165.278 kb on + strand, within GFF1563 at 165.279 kb on - strand, within GFF1563 at 165.279 kb on - strand, within GFF1563 at 165.279 kb on - strand, within GFF1563 at 165.431 kb on + strand, within GFF1564 at 165.431 kb on + strand, within GFF1564 at 165.431 kb on + strand, within GFF1564 at 165.431 kb on + strand, within GFF1564 at 165.431 kb on + strand, within GFF1564 at 165.431 kb on + strand, within GFF1564 at 165.432 kb on - strand, within GFF1564 at 165.432 kb on - strand, within GFF1564 at 165.432 kb on - strand, within GFF1564 at 165.432 kb on - strand, within GFF1564 at 165.432 kb on - strand, within GFF1564 at 165.432 kb on - strand, within GFF1564 at 165.432 kb on - strand, within GFF1564 at 165.495 kb on - strand at 165.495 kb on - strand at 165.540 kb on + strand at 165.540 kb on + strand at 165.540 kb on + strand at 165.541 kb on - strand at 165.541 kb on - strand at 165.603 kb on - strand at 165.639 kb on - strand at 165.644 kb on + strand at 165.644 kb on + strand at 165.645 kb on - strand at 165.645 kb on - strand at 165.645 kb on - strand at 165.645 kb on - strand at 165.648 kb on + strand at 165.648 kb on + strand at 165.648 kb on + strand at 165.648 kb on + strand at 165.648 kb on + strand at 165.649 kb on - strand at 165.649 kb on - strand at 165.651 kb on + strand at 165.651 kb on + strand at 165.651 kb on + strand at 165.651 kb on + strand at 165.651 kb on + strand at 165.651 kb on + strand at 165.652 kb on - strand at 165.652 kb on - strand at 165.652 kb on - strand at 165.652 kb on - strand at 165.652 kb on - strand at 165.652 kb on - strand at 165.652 kb on - strand at 165.731 kb on - strand at 165.731 kb on - strand at 165.731 kb on - strand at 165.732 kb on + strand at 165.732 kb on + strand at 165.732 kb on + strand at 165.732 kb on + strand at 165.732 kb on + strand at 165.732 kb on + strand at 165.733 kb on - strand at 165.733 kb on - strand at 165.733 kb on - strand at 165.733 kb on - strand at 165.733 kb on - strand at 165.756 kb on + strand at 165.757 kb on - strand at 165.776 kb on - strand at 165.866 kb on - strand, within GFF1565 at 165.866 kb on - strand, within GFF1565 at 166.024 kb on + strand, within GFF1565 at 166.025 kb on - strand, within GFF1565 at 166.025 kb on - strand, within GFF1565 at 166.025 kb on - strand, within GFF1565 at 166.222 kb on + strand, within GFF1565 at 166.222 kb on + strand, within GFF1565 at 166.291 kb on + strand, within GFF1565 at 166.291 kb on + strand, within GFF1565 at 166.292 kb on - strand, within GFF1565 at 166.292 kb on - strand, within GFF1565 at 166.387 kb on + strand, within GFF1565 at 166.387 kb on + strand, within GFF1565 at 166.387 kb on + strand, within GFF1565 at 166.388 kb on - strand, within GFF1565 at 166.388 kb on - strand, within GFF1565 at 166.388 kb on - strand, within GFF1565 at 166.388 kb on - strand, within GFF1565 at 166.388 kb on - strand, within GFF1565 at 166.388 kb on - strand, within GFF1565 at 166.388 kb on - strand, within GFF1565 at 166.388 kb on - strand, within GFF1565 at 166.461 kb on - strand, within GFF1565 at 166.540 kb on + strand, within GFF1565 at 166.562 kb on - strand, within GFF1565 at 166.562 kb on - strand, within GFF1565 at 166.646 kb on - strand, within GFF1565 at 166.783 kb on + strand, within GFF1565 at 166.784 kb on - strand, within GFF1565 at 166.903 kb on + strand at 166.904 kb on - strand at 166.904 kb on - strand at 166.904 kb on - strand at 166.904 kb on - strand at 167.029 kb on + strand at 167.029 kb on + strand at 167.046 kb on + strand at 167.115 kb on + strand at 167.115 kb on + strand at 167.115 kb on + strand at 167.116 kb on - strand at 167.247 kb on + strand at 167.252 kb on + strand at 167.252 kb on + strand at 167.252 kb on + strand at 167.253 kb on - strand at 167.253 kb on - strand at 167.256 kb on + strand at 167.257 kb on - strand at 167.314 kb on + strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.436 kb on + strand at 167.436 kb on + strand at 167.437 kb on - strand at 167.559 kb on + strand, within GFF1566 at 167.559 kb on + strand, within GFF1566 at 167.571 kb on + strand, within GFF1566 at 167.571 kb on + strand, within GFF1566 at 167.571 kb on + strand, within GFF1566 at 167.571 kb on + strand, within GFF1566 at 167.572 kb on - strand, within GFF1566 at 167.572 kb on - strand, within GFF1566 at 167.572 kb on - strand, within GFF1566 at 167.572 kb on - strand, within GFF1566 at 167.572 kb on - strand, within GFF1566 at 167.574 kb on + strand, within GFF1566 at 167.651 kb on + strand at 167.651 kb on + strand at 167.651 kb on + strand at 167.655 kb on + strand at 167.655 kb on + strand at 167.655 kb on + strand at 167.655 kb on + strand at 167.655 kb on + strand at 167.655 kb on + strand at 167.655 kb on + strand at 167.655 kb on + strand at 167.655 kb on + strand at 167.656 kb on - strand at 167.656 kb on - strand at 167.656 kb on - strand at 167.656 kb on - strand at 167.656 kb on - strand at 167.656 kb on - strand at 167.871 kb on + strand, within GFF1567 at 167.871 kb on + strand, within GFF1567 at 167.871 kb on + strand, within GFF1567 at 167.871 kb on + strand, within GFF1567 at 167.872 kb on - strand, within GFF1567 at 167.872 kb on - strand, within GFF1567
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 164,816 + GFF1562 0.89 +1.8 164,816 + GFF1562 0.89 -0.2 164,817 - GFF1562 0.89 -0.9 164,817 - GFF1562 0.89 -0.6 164,817 - GFF1562 0.89 -0.7 164,817 - GFF1562 0.89 -0.4 164,919 - -0.1 165,024 + -0.1 165,024 + -0.4 165,024 + -0.9 165,024 + -0.4 165,025 - -0.0 165,025 - -0.4 165,025 - -0.2 165,025 - -0.7 165,025 - -0.4 165,028 - +0.8 165,056 + +0.5 165,057 - -0.6 165,057 - +2.2 165,059 + +0.1 165,059 + -0.2 165,059 + -0.4 165,060 - +1.1 165,088 - -1.5 165,093 + -1.1 165,093 + -0.6 165,093 + -0.1 165,093 + +0.4 165,094 - -1.9 165,094 - -0.9 165,106 - -0.4 165,124 - GFF1563 0.16 -0.3 165,173 + GFF1563 0.37 -0.1 165,173 + GFF1563 0.37 -0.8 165,212 + GFF1563 0.54 +0.0 165,213 - GFF1563 0.54 +0.5 165,213 - GFF1563 0.54 +0.2 165,213 - GFF1563 0.54 -0.9 165,213 - GFF1563 0.54 +0.6 165,213 - GFF1563 0.54 +0.2 165,278 + GFF1563 0.82 -0.7 165,278 + GFF1563 0.82 -0.2 165,278 + GFF1563 0.82 -0.4 165,278 + GFF1563 0.82 -0.3 165,279 - GFF1563 0.82 -0.1 165,279 - GFF1563 0.82 -0.5 165,279 - GFF1563 0.82 -0.8 165,431 + GFF1564 0.74 -1.0 165,431 + GFF1564 0.74 -1.6 165,431 + GFF1564 0.74 -0.1 165,431 + GFF1564 0.74 -0.0 165,431 + GFF1564 0.74 -0.4 165,431 + GFF1564 0.74 +0.1 165,432 - GFF1564 0.75 -0.5 165,432 - GFF1564 0.75 +0.1 165,432 - GFF1564 0.75 -0.0 165,432 - GFF1564 0.75 -0.6 165,432 - GFF1564 0.75 -1.0 165,432 - GFF1564 0.75 -0.1 165,432 - GFF1564 0.75 -0.2 165,495 - +0.0 165,495 - -0.2 165,540 + -0.3 165,540 + -1.7 165,540 + -0.3 165,541 - -0.5 165,541 - -0.6 165,603 - -0.9 165,639 - -0.5 165,644 + -1.2 165,644 + -0.6 165,645 - -0.4 165,645 - +0.2 165,645 - -1.1 165,645 - -0.5 165,648 + +0.2 165,648 + -1.0 165,648 + +1.0 165,648 + -1.1 165,648 + -0.6 165,649 - -0.3 165,649 - +1.2 165,651 + -0.6 165,651 + -0.1 165,651 + -0.6 165,651 + -2.6 165,651 + +2.2 165,651 + +0.0 165,652 - +0.8 165,652 - -0.9 165,652 - -0.4 165,652 - -0.3 165,652 - -1.1 165,652 - +1.2 165,652 - +0.4 165,731 - +0.0 165,731 - +0.7 165,731 - +0.3 165,732 + -1.2 165,732 + -0.6 165,732 + -0.1 165,732 + +0.3 165,732 + -0.3 165,732 + -0.8 165,733 - +0.3 165,733 - -0.2 165,733 - -0.2 165,733 - -0.6 165,733 - -0.6 165,756 + -0.3 165,757 - -0.8 165,776 - +0.0 165,866 - GFF1565 0.11 -2.3 165,866 - GFF1565 0.11 -0.1 166,024 + GFF1565 0.24 -0.3 166,025 - GFF1565 0.24 +0.5 166,025 - GFF1565 0.24 +1.0 166,025 - GFF1565 0.24 +0.3 166,222 + GFF1565 0.41 +0.6 166,222 + GFF1565 0.41 -0.5 166,291 + GFF1565 0.47 -0.8 166,291 + GFF1565 0.47 -0.0 166,292 - GFF1565 0.47 +0.4 166,292 - GFF1565 0.47 -0.6 166,387 + GFF1565 0.55 +1.0 166,387 + GFF1565 0.55 +0.4 166,387 + GFF1565 0.55 +0.3 166,388 - GFF1565 0.55 -0.9 166,388 - GFF1565 0.55 -0.2 166,388 - GFF1565 0.55 -0.3 166,388 - GFF1565 0.55 +0.8 166,388 - GFF1565 0.55 -0.5 166,388 - GFF1565 0.55 +0.0 166,388 - GFF1565 0.55 -0.5 166,388 - GFF1565 0.55 +0.8 166,461 - GFF1565 0.62 -0.6 166,540 + GFF1565 0.69 -1.0 166,562 - GFF1565 0.70 +1.2 166,562 - GFF1565 0.70 -0.6 166,646 - GFF1565 0.78 -1.0 166,783 + GFF1565 0.89 +1.0 166,784 - GFF1565 0.90 -0.5 166,903 + -0.3 166,904 - +2.2 166,904 - -0.1 166,904 - -1.9 166,904 - -0.5 167,029 + -1.3 167,029 + -1.3 167,046 + -0.4 167,115 + +0.0 167,115 + -0.0 167,115 + -0.4 167,116 - -1.5 167,247 + +1.8 167,252 + +0.1 167,252 + -1.1 167,252 + +0.6 167,253 - +0.2 167,253 - -0.6 167,256 + +0.0 167,257 - -0.6 167,314 + -0.1 167,352 + -0.4 167,352 + -0.1 167,352 + -1.1 167,436 + -0.4 167,436 + -0.7 167,437 - +1.0 167,559 + GFF1566 0.51 -0.4 167,559 + GFF1566 0.51 +0.7 167,571 + GFF1566 0.57 -0.7 167,571 + GFF1566 0.57 -0.7 167,571 + GFF1566 0.57 -1.0 167,571 + GFF1566 0.57 -0.5 167,572 - GFF1566 0.57 -0.8 167,572 - GFF1566 0.57 -0.4 167,572 - GFF1566 0.57 -0.4 167,572 - GFF1566 0.57 -0.5 167,572 - GFF1566 0.57 -1.7 167,574 + GFF1566 0.58 +1.6 167,651 + +0.5 167,651 + +1.6 167,651 + +2.2 167,655 + +0.2 167,655 + +0.3 167,655 + -1.3 167,655 + +0.0 167,655 + -1.3 167,655 + +0.1 167,655 + +0.2 167,655 + -0.2 167,655 + -2.3 167,656 - +0.0 167,656 - -1.4 167,656 - +0.3 167,656 - -1.0 167,656 - -0.8 167,656 - -0.6 167,871 + GFF1567 0.21 +0.0 167,871 + GFF1567 0.21 +0.2 167,871 + GFF1567 0.21 -0.1 167,871 + GFF1567 0.21 +0.1 167,872 - GFF1567 0.21 -0.2 167,872 - GFF1567 0.21 -0.7
Or see this region's nucleotide sequence