Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1497 and GFF1498 overlap by 4 nucleotides GFF1498 and GFF1499 are separated by 6 nucleotides
GFF1497 - BUG/TctC family periplasmic protein, at 89,847 to 90,833
GFF1497
GFF1498 - putative 2-pyrone-4,6-dicarboxylic acid hydrolase, at 90,830 to 91,750
GFF1498
GFF1499 - BUG/TctC family periplasmic protein, at 91,757 to 92,743
GFF1499
Position (kb)
90
91
92 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 90.055 kb on + strand, within GFF1497 at 90.115 kb on - strand, within GFF1497 at 90.268 kb on + strand, within GFF1497 at 90.269 kb on - strand, within GFF1497 at 90.493 kb on + strand, within GFF1497 at 90.493 kb on + strand, within GFF1497 at 90.494 kb on - strand, within GFF1497 at 90.494 kb on - strand, within GFF1497 at 90.560 kb on - strand, within GFF1497 at 90.870 kb on + strand at 90.870 kb on + strand at 90.871 kb on - strand at 91.104 kb on + strand, within GFF1498 at 91.104 kb on + strand, within GFF1498 at 91.104 kb on + strand, within GFF1498 at 91.104 kb on + strand, within GFF1498 at 91.105 kb on - strand, within GFF1498 at 91.105 kb on - strand, within GFF1498 at 91.105 kb on - strand, within GFF1498 at 91.214 kb on + strand, within GFF1498 at 91.509 kb on + strand, within GFF1498 at 91.509 kb on + strand, within GFF1498 at 92.001 kb on + strand, within GFF1499 at 92.001 kb on + strand, within GFF1499 at 92.001 kb on + strand, within GFF1499 at 92.002 kb on - strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.178 kb on + strand, within GFF1499 at 92.178 kb on + strand, within GFF1499 at 92.179 kb on - strand, within GFF1499 at 92.179 kb on - strand, within GFF1499 at 92.179 kb on - strand, within GFF1499 at 92.265 kb on + strand, within GFF1499 at 92.404 kb on - strand, within GFF1499 at 92.419 kb on - strand, within GFF1499 at 92.469 kb on + strand, within GFF1499 at 92.470 kb on - strand, within GFF1499 at 92.653 kb on - strand at 92.653 kb on - strand at 92.737 kb on + strand at 92.737 kb on + strand at 92.737 kb on + strand at 92.738 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 90,055 + GFF1497 0.21 -0.6 90,115 - GFF1497 0.27 +0.0 90,268 + GFF1497 0.43 -0.4 90,269 - GFF1497 0.43 -1.5 90,493 + GFF1497 0.65 +1.9 90,493 + GFF1497 0.65 -0.1 90,494 - GFF1497 0.66 -2.5 90,494 - GFF1497 0.66 -0.4 90,560 - GFF1497 0.72 +0.8 90,870 + -0.5 90,870 + +0.6 90,871 - -0.1 91,104 + GFF1498 0.30 -0.1 91,104 + GFF1498 0.30 -1.2 91,104 + GFF1498 0.30 -0.8 91,104 + GFF1498 0.30 -0.9 91,105 - GFF1498 0.30 -0.3 91,105 - GFF1498 0.30 -0.2 91,105 - GFF1498 0.30 -0.6 91,214 + GFF1498 0.42 +0.2 91,509 + GFF1498 0.74 +0.6 91,509 + GFF1498 0.74 -0.2 92,001 + GFF1499 0.25 -0.5 92,001 + GFF1499 0.25 -0.0 92,001 + GFF1499 0.25 -0.8 92,002 - GFF1499 0.25 -0.9 92,124 + GFF1499 0.37 +1.2 92,124 + GFF1499 0.37 -1.8 92,124 + GFF1499 0.37 -0.9 92,124 + GFF1499 0.37 -2.5 92,124 + GFF1499 0.37 +0.6 92,124 + GFF1499 0.37 -1.1 92,124 + GFF1499 0.37 -1.5 92,124 + GFF1499 0.37 +1.2 92,124 + GFF1499 0.37 -0.3 92,124 + GFF1499 0.37 -0.6 92,124 + GFF1499 0.37 +0.0 92,124 + GFF1499 0.37 -1.0 92,124 + GFF1499 0.37 -0.1 92,124 + GFF1499 0.37 +0.1 92,124 + GFF1499 0.37 -0.3 92,125 - GFF1499 0.37 -0.6 92,125 - GFF1499 0.37 -0.4 92,125 - GFF1499 0.37 -0.4 92,125 - GFF1499 0.37 -1.1 92,125 - GFF1499 0.37 -0.2 92,125 - GFF1499 0.37 -0.1 92,125 - GFF1499 0.37 +0.2 92,125 - GFF1499 0.37 +0.2 92,178 + GFF1499 0.43 -0.6 92,178 + GFF1499 0.43 -0.1 92,179 - GFF1499 0.43 -1.0 92,179 - GFF1499 0.43 -1.1 92,179 - GFF1499 0.43 -0.1 92,265 + GFF1499 0.51 +1.4 92,404 - GFF1499 0.66 +2.2 92,419 - GFF1499 0.67 +1.5 92,469 + GFF1499 0.72 -2.0 92,470 - GFF1499 0.72 -1.3 92,653 - +0.9 92,653 - -0.1 92,737 + +0.4 92,737 + +0.2 92,737 + -0.7 92,738 - +1.5
Or see this region's nucleotide sequence