Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1475 and GFF1476 are separated by 89 nucleotides GFF1476 and GFF1477 are separated by 6 nucleotides GFF1477 and GFF1478 are separated by 45 nucleotides GFF1478 and GFF1479 are separated by 33 nucleotides
GFF1475 - no description, at 67,109 to 67,747
GFF1475
GFF1476 - hypothetical protein, at 67,837 to 68,583
GFF1476
GFF1477 - BUG/TctC family periplasmic protein, at 68,590 to 69,558
GFF1477
GFF1478 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31), at 69,604 to 70,482
GFF1478
GFF1479 - Aldehyde dehydrogenase (EC 1.2.1.3), at 70,516 to 72,012
GFF1479
Position (kb) 68
69
70 Strain fitness (log2 ratio) -1
0
1
2
3 at 67.807 kb on + strand at 67.807 kb on + strand at 67.808 kb on - strand at 67.808 kb on - strand at 67.849 kb on + strand at 67.849 kb on + strand at 67.850 kb on - strand at 67.953 kb on - strand, within GFF1476 at 67.953 kb on - strand, within GFF1476 at 68.068 kb on + strand, within GFF1476 at 68.069 kb on - strand, within GFF1476 at 68.069 kb on - strand, within GFF1476 at 68.069 kb on - strand, within GFF1476 at 68.069 kb on - strand, within GFF1476 at 68.072 kb on + strand, within GFF1476 at 68.072 kb on + strand, within GFF1476 at 68.073 kb on - strand, within GFF1476 at 68.252 kb on + strand, within GFF1476 at 68.252 kb on + strand, within GFF1476 at 68.253 kb on - strand, within GFF1476 at 68.273 kb on + strand, within GFF1476 at 68.274 kb on - strand, within GFF1476 at 68.310 kb on - strand, within GFF1476 at 68.318 kb on + strand, within GFF1476 at 68.318 kb on + strand, within GFF1476 at 68.330 kb on + strand, within GFF1476 at 68.571 kb on + strand at 68.571 kb on + strand at 68.572 kb on - strand at 69.128 kb on + strand, within GFF1477 at 69.129 kb on - strand, within GFF1477 at 69.129 kb on - strand, within GFF1477 at 69.129 kb on - strand, within GFF1477 at 69.197 kb on + strand, within GFF1477 at 69.237 kb on - strand, within GFF1477 at 69.677 kb on + strand at 69.678 kb on - strand at 69.836 kb on + strand, within GFF1478 at 70.238 kb on + strand, within GFF1478
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 67,807 + -0.8 67,807 + +0.0 67,808 - -0.7 67,808 - +0.9 67,849 + +1.4 67,849 + +0.2 67,850 - +0.2 67,953 - GFF1476 0.16 +1.8 67,953 - GFF1476 0.16 -0.1 68,068 + GFF1476 0.31 -0.9 68,069 - GFF1476 0.31 +0.4 68,069 - GFF1476 0.31 -0.3 68,069 - GFF1476 0.31 +0.2 68,069 - GFF1476 0.31 +2.8 68,072 + GFF1476 0.31 +1.1 68,072 + GFF1476 0.31 +0.2 68,073 - GFF1476 0.32 -1.0 68,252 + GFF1476 0.56 +0.5 68,252 + GFF1476 0.56 +0.3 68,253 - GFF1476 0.56 -0.3 68,273 + GFF1476 0.58 -0.1 68,274 - GFF1476 0.59 +0.8 68,310 - GFF1476 0.63 -0.5 68,318 + GFF1476 0.64 +1.0 68,318 + GFF1476 0.64 -0.1 68,330 + GFF1476 0.66 -0.1 68,571 + +0.0 68,571 + +1.8 68,572 - -1.3 69,128 + GFF1477 0.56 +1.2 69,129 - GFF1477 0.56 -0.8 69,129 - GFF1477 0.56 +0.4 69,129 - GFF1477 0.56 -0.1 69,197 + GFF1477 0.63 -0.2 69,237 - GFF1477 0.67 +1.8 69,677 + -1.1 69,678 - -0.5 69,836 + GFF1478 0.26 +0.2 70,238 + GFF1478 0.72 -1.1
Or see this region's nucleotide sequence