Strain Fitness in Variovorax sp. SCN45 around GFF1343

Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1342 and GFF1343 are separated by 31 nucleotidesGFF1343 and GFF1344 are separated by 46 nucleotides GFF1342 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 683,850 to 685,955 GFF1342 GFF1343 - macromolecule metabolism; macromolecule degradation; degradation of proteins, peptides, glycopeptides, at 685,987 to 687,462 GFF1343 GFF1344 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 687,509 to 688,684 GFF1344 Position (kb) 685 686 687 688Strain fitness (log2 ratio) -2 -1 0 1 2at 685.323 kb on + strand, within GFF1342at 685.323 kb on + strand, within GFF1342at 685.323 kb on + strand, within GFF1342at 685.323 kb on + strand, within GFF1342at 685.323 kb on + strand, within GFF1342at 685.324 kb on - strand, within GFF1342at 685.324 kb on - strand, within GFF1342at 685.324 kb on - strand, within GFF1342at 685.497 kb on + strand, within GFF1342at 685.498 kb on - strand, within GFF1342at 685.498 kb on - strand, within GFF1342at 685.498 kb on - strand, within GFF1342at 685.563 kb on + strand, within GFF1342at 685.569 kb on + strand, within GFF1342at 685.570 kb on - strand, within GFF1342at 685.695 kb on + strand, within GFF1342at 685.695 kb on + strand, within GFF1342at 685.695 kb on + strand, within GFF1342at 685.696 kb on - strand, within GFF1342at 685.696 kb on - strand, within GFF1342at 685.696 kb on - strand, within GFF1342at 685.770 kb on + strandat 685.770 kb on + strandat 685.770 kb on + strandat 685.771 kb on - strandat 685.771 kb on - strandat 685.771 kb on - strandat 685.818 kb on + strandat 685.818 kb on + strandat 685.819 kb on - strandat 685.839 kb on + strandat 685.839 kb on + strandat 685.839 kb on + strandat 685.839 kb on + strandat 685.840 kb on - strandat 685.840 kb on - strandat 686.221 kb on + strand, within GFF1343at 686.222 kb on - strand, within GFF1343at 686.293 kb on + strand, within GFF1343at 686.293 kb on + strand, within GFF1343at 686.294 kb on - strand, within GFF1343at 686.294 kb on - strand, within GFF1343at 686.425 kb on + strand, within GFF1343at 686.425 kb on + strand, within GFF1343at 686.530 kb on + strand, within GFF1343at 686.530 kb on + strand, within GFF1343at 686.530 kb on + strand, within GFF1343at 686.531 kb on - strand, within GFF1343at 686.661 kb on - strand, within GFF1343at 686.714 kb on - strand, within GFF1343at 687.022 kb on - strand, within GFF1343at 687.022 kb on - strand, within GFF1343at 687.152 kb on - strand, within GFF1343at 687.152 kb on - strand, within GFF1343at 687.397 kb on + strandat 687.398 kb on - strandat 687.418 kb on + strandat 687.418 kb on + strandat 687.418 kb on + strandat 687.418 kb on + strandat 687.418 kb on + strandat 687.418 kb on + strandat 687.419 kb on - strandat 687.842 kb on + strand, within GFF1344at 687.842 kb on + strand, within GFF1344at 687.842 kb on + strand, within GFF1344at 687.842 kb on + strand, within GFF1344at 687.842 kb on + strand, within GFF1344at 687.843 kb on - strand, within GFF1344at 687.843 kb on - strand, within GFF1344at 687.843 kb on - strand, within GFF1344at 687.843 kb on - strand, within GFF1344at 687.843 kb on - strand, within GFF1344at 687.843 kb on - strand, within GFF1344at 687.843 kb on - strand, within GFF1344at 687.843 kb on - strand, within GFF1344at 687.941 kb on + strand, within GFF1344at 687.941 kb on + strand, within GFF1344at 687.942 kb on - strand, within GFF1344at 687.942 kb on - strand, within GFF1344at 687.942 kb on - strand, within GFF1344at 687.942 kb on - strand, within GFF1344at 687.942 kb on - strand, within GFF1344at 687.984 kb on - strand, within GFF1344at 687.984 kb on - strand, within GFF1344at 688.013 kb on + strand, within GFF1344at 688.292 kb on + strand, within GFF1344at 688.292 kb on + strand, within GFF1344at 688.293 kb on - strand, within GFF1344at 688.293 kb on - strand, within GFF1344

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
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685,323 + GFF1342 0.70 -0.2
685,323 + GFF1342 0.70 +0.8
685,323 + GFF1342 0.70 -0.0
685,323 + GFF1342 0.70 +0.2
685,323 + GFF1342 0.70 +0.5
685,324 - GFF1342 0.70 -1.7
685,324 - GFF1342 0.70 -0.4
685,324 - GFF1342 0.70 +0.3
685,497 + GFF1342 0.78 -2.6
685,498 - GFF1342 0.78 -0.1
685,498 - GFF1342 0.78 -0.2
685,498 - GFF1342 0.78 -0.5
685,563 + GFF1342 0.81 +1.1
685,569 + GFF1342 0.82 -2.3
685,570 - GFF1342 0.82 +0.0
685,695 + GFF1342 0.88 -0.9
685,695 + GFF1342 0.88 -0.1
685,695 + GFF1342 0.88 +0.6
685,696 - GFF1342 0.88 -1.4
685,696 - GFF1342 0.88 +0.6
685,696 - GFF1342 0.88 -1.2
685,770 + -0.7
685,770 + -1.0
685,770 + +1.0
685,771 - -1.9
685,771 - -0.6
685,771 - +0.1
685,818 + +0.8
685,818 + -0.1
685,819 - -0.3
685,839 + +0.2
685,839 + +1.6
685,839 + -0.8
685,839 + -0.4
685,840 - +0.4
685,840 - +0.3
686,221 + GFF1343 0.16 -0.0
686,222 - GFF1343 0.16 +0.1
686,293 + GFF1343 0.21 +0.2
686,293 + GFF1343 0.21 -0.2
686,294 - GFF1343 0.21 +1.5
686,294 - GFF1343 0.21 -0.7
686,425 + GFF1343 0.30 +0.2
686,425 + GFF1343 0.30 -0.5
686,530 + GFF1343 0.37 +2.0
686,530 + GFF1343 0.37 -0.2
686,530 + GFF1343 0.37 +0.5
686,531 - GFF1343 0.37 -0.7
686,661 - GFF1343 0.46 -0.3
686,714 - GFF1343 0.49 -0.3
687,022 - GFF1343 0.70 -0.4
687,022 - GFF1343 0.70 -0.2
687,152 - GFF1343 0.79 +0.4
687,152 - GFF1343 0.79 -0.1
687,397 + -1.1
687,398 - +0.3
687,418 + +0.5
687,418 + -0.2
687,418 + +0.8
687,418 + +0.2
687,418 + +0.6
687,418 + -0.8
687,419 - -1.6
687,842 + GFF1344 0.28 +0.6
687,842 + GFF1344 0.28 +0.5
687,842 + GFF1344 0.28 -0.5
687,842 + GFF1344 0.28 -0.8
687,842 + GFF1344 0.28 +1.5
687,843 - GFF1344 0.28 +0.2
687,843 - GFF1344 0.28 -0.8
687,843 - GFF1344 0.28 +0.0
687,843 - GFF1344 0.28 -1.0
687,843 - GFF1344 0.28 -0.1
687,843 - GFF1344 0.28 -0.5
687,843 - GFF1344 0.28 -0.2
687,843 - GFF1344 0.28 -1.5
687,941 + GFF1344 0.37 -0.5
687,941 + GFF1344 0.37 -0.9
687,942 - GFF1344 0.37 -1.1
687,942 - GFF1344 0.37 +0.3
687,942 - GFF1344 0.37 -0.4
687,942 - GFF1344 0.37 -1.0
687,942 - GFF1344 0.37 +0.5
687,984 - GFF1344 0.40 +0.1
687,984 - GFF1344 0.40 +0.8
688,013 + GFF1344 0.43 +2.2
688,292 + GFF1344 0.67 +0.5
688,292 + GFF1344 0.67 -1.9
688,293 - GFF1344 0.67 -0.5
688,293 - GFF1344 0.67 +0.2

Or see this region's nucleotide sequence