Strain Fitness in Variovorax sp. SCN45 around GFF124

Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF122 and GFF123 overlap by 8 nucleotidesGFF123 and GFF124 are separated by 16 nucleotidesGFF124 and GFF125 overlap by 4 nucleotides GFF122 - Dihydroorotate dehydrogenase electron transfer subunit (EC 1.3.3.1), at 150,815 to 151,690 GFF122 GFF123 - Dihydroorotate dehydrogenase (NAD(+)), catalytic subunit (EC 1.3.1.14), at 151,683 to 152,633 GFF123 GFF124 - Beta-ureidopropionase (EC 3.5.1.6), at 152,650 to 153,930 GFF124 GFF125 - Dihydroorotase (EC 3.5.2.3), at 153,927 to 154,979 GFF125 Position (kb) 152 153 154Strain fitness (log2 ratio) -2 -1 0 1at 151.764 kb on + strandat 151.915 kb on - strand, within GFF123at 151.917 kb on + strand, within GFF123at 151.941 kb on + strand, within GFF123at 152.115 kb on + strand, within GFF123at 152.115 kb on + strand, within GFF123at 152.116 kb on - strand, within GFF123at 152.368 kb on - strand, within GFF123at 152.481 kb on - strand, within GFF123at 152.527 kb on - strand, within GFF123at 152.759 kb on - strandat 152.789 kb on - strand, within GFF124at 152.907 kb on + strand, within GFF124at 153.067 kb on + strand, within GFF124at 153.067 kb on + strand, within GFF124at 153.067 kb on + strand, within GFF124at 153.068 kb on - strand, within GFF124at 153.068 kb on - strand, within GFF124at 153.068 kb on - strand, within GFF124at 153.068 kb on - strand, within GFF124at 153.233 kb on - strand, within GFF124at 153.233 kb on - strand, within GFF124at 153.590 kb on - strand, within GFF124at 154.108 kb on - strand, within GFF125at 154.596 kb on + strand, within GFF125at 154.596 kb on + strand, within GFF125at 154.596 kb on + strand, within GFF125at 154.770 kb on + strand, within GFF125at 154.771 kb on - strand, within GFF125

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
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151,764 + +0.1
151,915 - GFF123 0.24 -0.1
151,917 + GFF123 0.25 -0.3
151,941 + GFF123 0.27 -0.1
152,115 + GFF123 0.45 +0.6
152,115 + GFF123 0.45 -1.0
152,116 - GFF123 0.46 -0.8
152,368 - GFF123 0.72 +0.4
152,481 - GFF123 0.84 -0.6
152,527 - GFF123 0.89 +0.2
152,759 - -0.0
152,789 - GFF124 0.11 -0.2
152,907 + GFF124 0.20 +0.4
153,067 + GFF124 0.33 +0.1
153,067 + GFF124 0.33 +1.0
153,067 + GFF124 0.33 +1.0
153,068 - GFF124 0.33 +1.1
153,068 - GFF124 0.33 +0.2
153,068 - GFF124 0.33 +0.1
153,068 - GFF124 0.33 -1.0
153,233 - GFF124 0.46 -1.3
153,233 - GFF124 0.46 -0.3
153,590 - GFF124 0.73 -1.9
154,108 - GFF125 0.17 -0.2
154,596 + GFF125 0.64 +0.1
154,596 + GFF125 0.64 -0.1
154,596 + GFF125 0.64 -0.2
154,770 + GFF125 0.80 -1.2
154,771 - GFF125 0.80 +0.1

Or see this region's nucleotide sequence