Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1115 and GFF1116 are separated by 17 nucleotides GFF1116 and GFF1118 overlap by 4 nucleotides GFF1118 and GFF1117 overlap by 2 nucleotides
GFF1115 - Putative transmembrane protein, at 431,796 to 432,980
GFF1115
GFF1116 - Membrane-bound lytic murein transglycosylase D, at 432,998 to 434,590
GFF1116
GFF1118 - Hydroxyacylglutathione hydrolase (EC 3.1.2.6), at 434,587 to 435,366
GFF1118
GFF1117 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 435,365 to 436,135
GFF1117
Position (kb) 432
433
434
435 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 432.324 kb on + strand, within GFF1115 at 432.325 kb on - strand, within GFF1115 at 432.325 kb on - strand, within GFF1115 at 432.558 kb on + strand, within GFF1115 at 432.558 kb on + strand, within GFF1115 at 432.558 kb on + strand, within GFF1115 at 432.559 kb on - strand, within GFF1115 at 432.559 kb on - strand, within GFF1115 at 432.559 kb on - strand, within GFF1115 at 432.639 kb on + strand, within GFF1115 at 432.639 kb on + strand, within GFF1115 at 432.639 kb on + strand, within GFF1115 at 432.639 kb on + strand, within GFF1115 at 432.640 kb on - strand, within GFF1115 at 432.732 kb on + strand, within GFF1115 at 432.732 kb on + strand, within GFF1115 at 432.765 kb on + strand, within GFF1115 at 432.765 kb on + strand, within GFF1115 at 432.765 kb on + strand, within GFF1115 at 432.765 kb on + strand, within GFF1115 at 432.765 kb on + strand, within GFF1115 at 432.765 kb on + strand, within GFF1115 at 432.766 kb on - strand, within GFF1115 at 432.766 kb on - strand, within GFF1115 at 432.829 kb on - strand, within GFF1115 at 432.997 kb on - strand at 433.200 kb on + strand, within GFF1116 at 433.200 kb on + strand, within GFF1116 at 433.200 kb on + strand, within GFF1116 at 433.200 kb on + strand, within GFF1116 at 433.201 kb on - strand, within GFF1116 at 433.201 kb on - strand, within GFF1116 at 433.201 kb on - strand, within GFF1116 at 433.201 kb on - strand, within GFF1116 at 433.215 kb on + strand, within GFF1116 at 433.284 kb on + strand, within GFF1116 at 433.284 kb on + strand, within GFF1116 at 433.285 kb on - strand, within GFF1116 at 433.285 kb on - strand, within GFF1116 at 433.285 kb on - strand, within GFF1116 at 433.572 kb on + strand, within GFF1116 at 433.573 kb on - strand, within GFF1116 at 433.573 kb on - strand, within GFF1116 at 433.573 kb on - strand, within GFF1116 at 433.587 kb on + strand, within GFF1116 at 433.588 kb on - strand, within GFF1116 at 433.588 kb on - strand, within GFF1116 at 433.776 kb on + strand, within GFF1116 at 433.776 kb on + strand, within GFF1116 at 433.776 kb on + strand, within GFF1116 at 433.776 kb on + strand, within GFF1116 at 433.777 kb on - strand, within GFF1116 at 433.777 kb on - strand, within GFF1116 at 433.777 kb on - strand, within GFF1116 at 433.777 kb on - strand, within GFF1116 at 433.777 kb on - strand, within GFF1116 at 433.863 kb on + strand, within GFF1116 at 433.863 kb on + strand, within GFF1116 at 433.863 kb on + strand, within GFF1116 at 433.863 kb on + strand, within GFF1116 at 433.863 kb on + strand, within GFF1116 at 433.863 kb on + strand, within GFF1116 at 433.863 kb on + strand, within GFF1116 at 433.863 kb on + strand, within GFF1116 at 433.863 kb on + strand, within GFF1116 at 433.863 kb on + strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.864 kb on - strand, within GFF1116 at 433.899 kb on + strand, within GFF1116 at 433.899 kb on + strand, within GFF1116 at 433.899 kb on + strand, within GFF1116 at 433.899 kb on + strand, within GFF1116 at 433.899 kb on + strand, within GFF1116 at 433.900 kb on - strand, within GFF1116 at 433.900 kb on - strand, within GFF1116 at 433.900 kb on - strand, within GFF1116 at 433.900 kb on - strand, within GFF1116 at 433.968 kb on + strand, within GFF1116 at 433.968 kb on + strand, within GFF1116 at 433.969 kb on - strand, within GFF1116 at 433.969 kb on - strand, within GFF1116 at 433.969 kb on - strand, within GFF1116 at 434.007 kb on + strand, within GFF1116 at 434.007 kb on + strand, within GFF1116 at 434.007 kb on + strand, within GFF1116 at 434.008 kb on - strand, within GFF1116 at 434.022 kb on + strand, within GFF1116 at 434.022 kb on + strand, within GFF1116 at 434.023 kb on - strand, within GFF1116 at 434.182 kb on - strand, within GFF1116 at 434.182 kb on - strand, within GFF1116 at 434.182 kb on - strand, within GFF1116 at 434.182 kb on - strand, within GFF1116 at 434.182 kb on - strand, within GFF1116 at 434.182 kb on - strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.247 kb on + strand, within GFF1116 at 434.248 kb on - strand, within GFF1116 at 434.248 kb on - strand, within GFF1116 at 434.248 kb on - strand, within GFF1116 at 434.248 kb on - strand, within GFF1116 at 434.248 kb on - strand, within GFF1116 at 434.248 kb on - strand, within GFF1116 at 434.281 kb on - strand, within GFF1116 at 434.281 kb on - strand, within GFF1116 at 434.281 kb on - strand, within GFF1116 at 434.281 kb on - strand, within GFF1116 at 434.281 kb on - strand, within GFF1116 at 434.298 kb on + strand, within GFF1116 at 434.298 kb on + strand, within GFF1116 at 434.298 kb on + strand, within GFF1116 at 434.298 kb on + strand, within GFF1116 at 434.298 kb on + strand, within GFF1116 at 434.299 kb on - strand, within GFF1116 at 434.299 kb on - strand, within GFF1116 at 434.299 kb on - strand, within GFF1116 at 434.347 kb on - strand, within GFF1116 at 434.455 kb on - strand at 434.792 kb on + strand, within GFF1118 at 434.792 kb on + strand, within GFF1118 at 434.792 kb on + strand, within GFF1118 at 434.793 kb on - strand, within GFF1118 at 434.793 kb on - strand, within GFF1118 at 434.793 kb on - strand, within GFF1118 at 434.844 kb on + strand, within GFF1118 at 434.845 kb on - strand, within GFF1118 at 434.845 kb on - strand, within GFF1118 at 434.855 kb on + strand, within GFF1118 at 434.855 kb on + strand, within GFF1118 at 434.855 kb on + strand, within GFF1118 at 434.856 kb on - strand, within GFF1118 at 434.856 kb on - strand, within GFF1118 at 435.023 kb on + strand, within GFF1118 at 435.023 kb on + strand, within GFF1118 at 435.023 kb on + strand, within GFF1118 at 435.023 kb on + strand, within GFF1118 at 435.024 kb on - strand, within GFF1118 at 435.024 kb on - strand, within GFF1118 at 435.024 kb on - strand, within GFF1118 at 435.043 kb on - strand, within GFF1118 at 435.053 kb on - strand, within GFF1118 at 435.053 kb on - strand, within GFF1118 at 435.053 kb on - strand, within GFF1118 at 435.116 kb on + strand, within GFF1118 at 435.116 kb on + strand, within GFF1118 at 435.117 kb on - strand, within GFF1118 at 435.220 kb on + strand, within GFF1118 at 435.220 kb on + strand, within GFF1118 at 435.220 kb on + strand, within GFF1118 at 435.329 kb on + strand at 435.329 kb on + strand at 435.329 kb on + strand at 435.329 kb on + strand at 435.329 kb on + strand at 435.329 kb on + strand at 435.329 kb on + strand at 435.329 kb on + strand at 435.329 kb on + strand at 435.329 kb on + strand at 435.329 kb on + strand at 435.329 kb on + strand at 435.329 kb on + strand at 435.329 kb on + strand at 435.329 kb on + strand at 435.329 kb on + strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.330 kb on - strand at 435.379 kb on + strand at 435.380 kb on - strand at 435.381 kb on + strand at 435.381 kb on + strand at 435.419 kb on + strand at 435.419 kb on + strand at 435.419 kb on + strand at 435.420 kb on - strand at 435.420 kb on - strand at 435.420 kb on - strand at 435.420 kb on - strand at 435.476 kb on + strand, within GFF1117 at 435.476 kb on + strand, within GFF1117 at 435.477 kb on - strand, within GFF1117
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 432,324 + GFF1115 0.45 -0.9 432,325 - GFF1115 0.45 -0.9 432,325 - GFF1115 0.45 +0.9 432,558 + GFF1115 0.64 +0.8 432,558 + GFF1115 0.64 -1.5 432,558 + GFF1115 0.64 +1.6 432,559 - GFF1115 0.64 +0.2 432,559 - GFF1115 0.64 +0.6 432,559 - GFF1115 0.64 -1.9 432,639 + GFF1115 0.71 -0.1 432,639 + GFF1115 0.71 -1.6 432,639 + GFF1115 0.71 -0.6 432,639 + GFF1115 0.71 +0.1 432,640 - GFF1115 0.71 +0.3 432,732 + GFF1115 0.79 -0.1 432,732 + GFF1115 0.79 +0.7 432,765 + GFF1115 0.82 -0.6 432,765 + GFF1115 0.82 -0.3 432,765 + GFF1115 0.82 +1.4 432,765 + GFF1115 0.82 +1.3 432,765 + GFF1115 0.82 -2.0 432,765 + GFF1115 0.82 -0.6 432,766 - GFF1115 0.82 +1.3 432,766 - GFF1115 0.82 -1.8 432,829 - GFF1115 0.87 -0.0 432,997 - +0.6 433,200 + GFF1116 0.13 +0.1 433,200 + GFF1116 0.13 +0.1 433,200 + GFF1116 0.13 -1.4 433,200 + GFF1116 0.13 +0.0 433,201 - GFF1116 0.13 -0.4 433,201 - GFF1116 0.13 +0.3 433,201 - GFF1116 0.13 +0.4 433,201 - GFF1116 0.13 -0.6 433,215 + GFF1116 0.14 -0.9 433,284 + GFF1116 0.18 +1.2 433,284 + GFF1116 0.18 -0.4 433,285 - GFF1116 0.18 -2.0 433,285 - GFF1116 0.18 +1.3 433,285 - GFF1116 0.18 -0.3 433,572 + GFF1116 0.36 -0.2 433,573 - GFF1116 0.36 -1.5 433,573 - GFF1116 0.36 -0.7 433,573 - GFF1116 0.36 +0.3 433,587 + GFF1116 0.37 -1.1 433,588 - GFF1116 0.37 -1.0 433,588 - GFF1116 0.37 +0.3 433,776 + GFF1116 0.49 -1.8 433,776 + GFF1116 0.49 -0.3 433,776 + GFF1116 0.49 +0.1 433,776 + GFF1116 0.49 +0.9 433,777 - GFF1116 0.49 -0.7 433,777 - GFF1116 0.49 -0.7 433,777 - GFF1116 0.49 +1.1 433,777 - GFF1116 0.49 +0.7 433,777 - GFF1116 0.49 -0.6 433,863 + GFF1116 0.54 -1.4 433,863 + GFF1116 0.54 -0.9 433,863 + GFF1116 0.54 -0.5 433,863 + GFF1116 0.54 -2.2 433,863 + GFF1116 0.54 -0.5 433,863 + GFF1116 0.54 -0.2 433,863 + GFF1116 0.54 -0.3 433,863 + GFF1116 0.54 -0.2 433,863 + GFF1116 0.54 -0.6 433,863 + GFF1116 0.54 -1.3 433,864 - GFF1116 0.54 -0.9 433,864 - GFF1116 0.54 +0.1 433,864 - GFF1116 0.54 -0.4 433,864 - GFF1116 0.54 +0.1 433,864 - GFF1116 0.54 +0.1 433,864 - GFF1116 0.54 +0.2 433,864 - GFF1116 0.54 +1.3 433,864 - GFF1116 0.54 -0.6 433,864 - GFF1116 0.54 +1.4 433,864 - GFF1116 0.54 +0.1 433,864 - GFF1116 0.54 -0.1 433,864 - GFF1116 0.54 -0.8 433,864 - GFF1116 0.54 +1.0 433,864 - GFF1116 0.54 +0.3 433,864 - GFF1116 0.54 -1.6 433,899 + GFF1116 0.57 +1.2 433,899 + GFF1116 0.57 -1.7 433,899 + GFF1116 0.57 +0.5 433,899 + GFF1116 0.57 +0.5 433,899 + GFF1116 0.57 +1.1 433,900 - GFF1116 0.57 +0.3 433,900 - GFF1116 0.57 +0.1 433,900 - GFF1116 0.57 +0.7 433,900 - GFF1116 0.57 +0.1 433,968 + GFF1116 0.61 -0.5 433,968 + GFF1116 0.61 -0.2 433,969 - GFF1116 0.61 -0.3 433,969 - GFF1116 0.61 -0.4 433,969 - GFF1116 0.61 +0.4 434,007 + GFF1116 0.63 -0.9 434,007 + GFF1116 0.63 +0.2 434,007 + GFF1116 0.63 +0.8 434,008 - GFF1116 0.63 +0.8 434,022 + GFF1116 0.64 -1.6 434,022 + GFF1116 0.64 -1.9 434,023 - GFF1116 0.64 -0.5 434,182 - GFF1116 0.74 +0.4 434,182 - GFF1116 0.74 -0.2 434,182 - GFF1116 0.74 +0.3 434,182 - GFF1116 0.74 +0.5 434,182 - GFF1116 0.74 -0.6 434,182 - GFF1116 0.74 +0.2 434,247 + GFF1116 0.78 +0.9 434,247 + GFF1116 0.78 +0.2 434,247 + GFF1116 0.78 -0.1 434,247 + GFF1116 0.78 +0.7 434,247 + GFF1116 0.78 -0.9 434,247 + GFF1116 0.78 -0.6 434,247 + GFF1116 0.78 -0.4 434,247 + GFF1116 0.78 -0.2 434,247 + GFF1116 0.78 -0.9 434,247 + GFF1116 0.78 -1.3 434,247 + GFF1116 0.78 -0.1 434,247 + GFF1116 0.78 -0.8 434,247 + GFF1116 0.78 -0.9 434,247 + GFF1116 0.78 +0.2 434,247 + GFF1116 0.78 +1.9 434,247 + GFF1116 0.78 -1.6 434,248 - GFF1116 0.78 +0.1 434,248 - GFF1116 0.78 +0.1 434,248 - GFF1116 0.78 -0.3 434,248 - GFF1116 0.78 +0.1 434,248 - GFF1116 0.78 -0.5 434,248 - GFF1116 0.78 -2.7 434,281 - GFF1116 0.81 -0.7 434,281 - GFF1116 0.81 -1.6 434,281 - GFF1116 0.81 +0.0 434,281 - GFF1116 0.81 -0.1 434,281 - GFF1116 0.81 +0.3 434,298 + GFF1116 0.82 +1.1 434,298 + GFF1116 0.82 +0.9 434,298 + GFF1116 0.82 -0.6 434,298 + GFF1116 0.82 +0.3 434,298 + GFF1116 0.82 +1.1 434,299 - GFF1116 0.82 +0.2 434,299 - GFF1116 0.82 -0.5 434,299 - GFF1116 0.82 -0.4 434,347 - GFF1116 0.85 +1.8 434,455 - -0.8 434,792 + GFF1118 0.26 -0.1 434,792 + GFF1118 0.26 -1.4 434,792 + GFF1118 0.26 -0.4 434,793 - GFF1118 0.26 -0.6 434,793 - GFF1118 0.26 +0.3 434,793 - GFF1118 0.26 +0.2 434,844 + GFF1118 0.33 -1.0 434,845 - GFF1118 0.33 -0.6 434,845 - GFF1118 0.33 -0.7 434,855 + GFF1118 0.34 -0.3 434,855 + GFF1118 0.34 +1.3 434,855 + GFF1118 0.34 -0.1 434,856 - GFF1118 0.34 -0.7 434,856 - GFF1118 0.34 +0.2 435,023 + GFF1118 0.56 -0.8 435,023 + GFF1118 0.56 -1.1 435,023 + GFF1118 0.56 +0.2 435,023 + GFF1118 0.56 -0.1 435,024 - GFF1118 0.56 +0.4 435,024 - GFF1118 0.56 +0.3 435,024 - GFF1118 0.56 +0.1 435,043 - GFF1118 0.58 -0.5 435,053 - GFF1118 0.60 +0.2 435,053 - GFF1118 0.60 +0.7 435,053 - GFF1118 0.60 +0.9 435,116 + GFF1118 0.68 -0.8 435,116 + GFF1118 0.68 -1.4 435,117 - GFF1118 0.68 -1.4 435,220 + GFF1118 0.81 -0.8 435,220 + GFF1118 0.81 -0.3 435,220 + GFF1118 0.81 +0.6 435,329 + -0.9 435,329 + +0.3 435,329 + +0.3 435,329 + -0.0 435,329 + -0.0 435,329 + +0.6 435,329 + +0.4 435,329 + -1.1 435,329 + +0.1 435,329 + +0.1 435,329 + -0.4 435,329 + +1.0 435,329 + -0.8 435,329 + +1.3 435,329 + +0.1 435,329 + -0.5 435,330 - +1.6 435,330 - +1.1 435,330 - -0.2 435,330 - -1.4 435,330 - -0.2 435,330 - +2.1 435,330 - -0.9 435,330 - -1.3 435,330 - -0.3 435,330 - +0.0 435,330 - -1.0 435,330 - -2.4 435,330 - -1.5 435,330 - +0.3 435,330 - -0.9 435,330 - -1.8 435,330 - -1.1 435,330 - -3.0 435,379 + +0.3 435,380 - -1.2 435,381 + +0.6 435,381 + -1.0 435,419 + -0.2 435,419 + -0.9 435,419 + -0.4 435,420 - +1.4 435,420 - -0.5 435,420 - -0.6 435,420 - -1.4 435,476 + GFF1117 0.14 -0.8 435,476 + GFF1117 0.14 -0.5 435,477 - GFF1117 0.15 -0.3
Or see this region's nucleotide sequence