Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1108 and GFF1109 are separated by 56 nucleotides GFF1109 and GFF1110 are separated by 7 nucleotides GFF1110 and GFF1111 are separated by 27 nucleotides GFF1111 and GFF1112 are separated by 137 nucleotides
GFF1108 - DNA polymerase III subunits gamma and tau (EC 2.7.7.7), at 425,554 to 427,425
GFF1108
GFF1109 - Nucleoid-associated protein YaaK, at 427,482 to 427,814
GFF1109
GFF1110 - Recombination protein RecR, at 427,822 to 428,412
GFF1110
GFF1111 - probable membrane protein NMA1176, at 428,440 to 428,832
GFF1111
GFF1112 - Hydroxymethylpyrimidine ABC transporter, substrate-binding component, at 428,970 to 430,007
GFF1112
Position (kb) 427
428
429 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 427.440 kb on + strand at 427.440 kb on + strand at 427.440 kb on + strand at 427.441 kb on - strand at 427.812 kb on + strand at 428.131 kb on + strand, within GFF1110 at 428.131 kb on + strand, within GFF1110 at 428.132 kb on - strand, within GFF1110 at 428.132 kb on - strand, within GFF1110 at 428.132 kb on - strand, within GFF1110 at 428.132 kb on - strand, within GFF1110 at 428.132 kb on - strand, within GFF1110 at 428.132 kb on - strand, within GFF1110 at 428.132 kb on - strand, within GFF1110 at 428.365 kb on + strand at 428.365 kb on + strand at 428.365 kb on + strand at 428.366 kb on - strand at 428.366 kb on - strand at 428.467 kb on + strand at 428.467 kb on + strand at 428.467 kb on + strand at 428.467 kb on + strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.501 kb on - strand, within GFF1111 at 428.713 kb on + strand, within GFF1111 at 428.713 kb on + strand, within GFF1111 at 428.713 kb on + strand, within GFF1111 at 428.713 kb on + strand, within GFF1111 at 428.713 kb on + strand, within GFF1111 at 428.713 kb on + strand, within GFF1111 at 428.714 kb on - strand, within GFF1111 at 428.714 kb on - strand, within GFF1111 at 428.714 kb on - strand, within GFF1111 at 428.714 kb on - strand, within GFF1111 at 428.714 kb on - strand, within GFF1111 at 428.734 kb on + strand, within GFF1111 at 428.734 kb on + strand, within GFF1111 at 428.734 kb on + strand, within GFF1111 at 428.734 kb on + strand, within GFF1111 at 428.734 kb on + strand, within GFF1111 at 428.734 kb on + strand, within GFF1111 at 428.735 kb on - strand, within GFF1111 at 428.735 kb on - strand, within GFF1111 at 428.735 kb on - strand, within GFF1111 at 428.735 kb on - strand, within GFF1111 at 428.735 kb on - strand, within GFF1111 at 428.746 kb on + strand, within GFF1111 at 428.746 kb on + strand, within GFF1111 at 428.747 kb on - strand, within GFF1111 at 428.747 kb on - strand, within GFF1111 at 428.747 kb on - strand, within GFF1111 at 428.747 kb on - strand, within GFF1111 at 428.747 kb on - strand, within GFF1111 at 428.830 kb on + strand at 428.830 kb on + strand at 428.869 kb on + strand at 428.869 kb on + strand at 428.869 kb on + strand at 428.869 kb on + strand at 428.869 kb on + strand at 428.869 kb on + strand at 428.870 kb on - strand at 428.870 kb on - strand at 428.921 kb on + strand at 428.921 kb on + strand at 428.922 kb on - strand at 429.105 kb on + strand, within GFF1112 at 429.105 kb on + strand, within GFF1112 at 429.105 kb on + strand, within GFF1112 at 429.105 kb on + strand, within GFF1112 at 429.105 kb on + strand, within GFF1112 at 429.106 kb on - strand, within GFF1112 at 429.106 kb on - strand, within GFF1112 at 429.108 kb on + strand, within GFF1112 at 429.108 kb on + strand, within GFF1112 at 429.108 kb on + strand, within GFF1112 at 429.108 kb on + strand, within GFF1112 at 429.108 kb on + strand, within GFF1112 at 429.109 kb on - strand, within GFF1112 at 429.109 kb on - strand, within GFF1112 at 429.141 kb on + strand, within GFF1112 at 429.141 kb on + strand, within GFF1112 at 429.142 kb on - strand, within GFF1112 at 429.142 kb on - strand, within GFF1112 at 429.145 kb on + strand, within GFF1112 at 429.247 kb on - strand, within GFF1112 at 429.247 kb on - strand, within GFF1112 at 429.247 kb on - strand, within GFF1112 at 429.357 kb on + strand, within GFF1112 at 429.357 kb on + strand, within GFF1112 at 429.358 kb on - strand, within GFF1112 at 429.358 kb on - strand, within GFF1112 at 429.359 kb on + strand, within GFF1112 at 429.359 kb on + strand, within GFF1112 at 429.360 kb on - strand, within GFF1112
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 427,440 + -0.7 427,440 + -0.1 427,440 + -0.0 427,441 - -0.4 427,812 + +0.6 428,131 + GFF1110 0.52 +0.1 428,131 + GFF1110 0.52 +0.7 428,132 - GFF1110 0.52 -0.7 428,132 - GFF1110 0.52 +3.2 428,132 - GFF1110 0.52 +0.1 428,132 - GFF1110 0.52 -0.0 428,132 - GFF1110 0.52 -0.8 428,132 - GFF1110 0.52 +0.7 428,132 - GFF1110 0.52 -2.4 428,365 + +0.9 428,365 + +0.0 428,365 + +0.2 428,366 - -0.5 428,366 - -2.2 428,467 + +3.5 428,467 + -0.9 428,467 + +0.4 428,467 + -0.5 428,468 - -0.3 428,468 - -0.1 428,468 - -0.7 428,468 - -0.7 428,468 - +0.3 428,468 - -0.3 428,468 - -0.5 428,468 - +0.9 428,468 - +0.8 428,501 - GFF1111 0.16 +0.9 428,713 + GFF1111 0.69 -0.3 428,713 + GFF1111 0.69 -0.8 428,713 + GFF1111 0.69 -0.0 428,713 + GFF1111 0.69 +0.5 428,713 + GFF1111 0.69 -1.4 428,713 + GFF1111 0.69 -0.6 428,714 - GFF1111 0.70 -0.1 428,714 - GFF1111 0.70 -0.1 428,714 - GFF1111 0.70 -0.6 428,714 - GFF1111 0.70 +0.6 428,714 - GFF1111 0.70 -0.4 428,734 + GFF1111 0.75 +0.6 428,734 + GFF1111 0.75 -1.6 428,734 + GFF1111 0.75 -0.3 428,734 + GFF1111 0.75 +0.2 428,734 + GFF1111 0.75 +0.4 428,734 + GFF1111 0.75 +1.5 428,735 - GFF1111 0.75 +0.2 428,735 - GFF1111 0.75 +0.6 428,735 - GFF1111 0.75 +0.1 428,735 - GFF1111 0.75 -0.5 428,735 - GFF1111 0.75 +0.4 428,746 + GFF1111 0.78 +0.6 428,746 + GFF1111 0.78 +1.9 428,747 - GFF1111 0.78 -0.3 428,747 - GFF1111 0.78 +0.0 428,747 - GFF1111 0.78 -0.4 428,747 - GFF1111 0.78 -0.9 428,747 - GFF1111 0.78 +0.1 428,830 + -1.3 428,830 + -0.8 428,869 + +1.9 428,869 + -1.0 428,869 + -1.1 428,869 + +1.5 428,869 + +0.2 428,869 + -2.4 428,870 - -0.3 428,870 - +0.3 428,921 + +1.3 428,921 + -0.1 428,922 - -0.5 429,105 + GFF1112 0.13 -0.7 429,105 + GFF1112 0.13 -0.7 429,105 + GFF1112 0.13 +0.2 429,105 + GFF1112 0.13 +0.4 429,105 + GFF1112 0.13 +0.3 429,106 - GFF1112 0.13 -0.2 429,106 - GFF1112 0.13 -0.2 429,108 + GFF1112 0.13 +0.4 429,108 + GFF1112 0.13 -0.3 429,108 + GFF1112 0.13 -0.0 429,108 + GFF1112 0.13 +1.1 429,108 + GFF1112 0.13 +1.7 429,109 - GFF1112 0.13 -2.1 429,109 - GFF1112 0.13 -0.9 429,141 + GFF1112 0.16 +0.4 429,141 + GFF1112 0.16 +0.3 429,142 - GFF1112 0.17 +1.9 429,142 - GFF1112 0.17 -0.7 429,145 + GFF1112 0.17 -0.3 429,247 - GFF1112 0.27 +0.7 429,247 - GFF1112 0.27 -0.3 429,247 - GFF1112 0.27 +1.9 429,357 + GFF1112 0.37 -0.3 429,357 + GFF1112 0.37 -0.6 429,358 - GFF1112 0.37 +0.9 429,358 - GFF1112 0.37 +2.2 429,359 + GFF1112 0.37 +1.0 429,359 + GFF1112 0.37 +1.1 429,360 - GFF1112 0.38 +0.5
Or see this region's nucleotide sequence