Strain Fitness in Variovorax sp. SCN45 around GFF1

Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1 and GFF2 are separated by 80 nucleotides GFF1 - hypothetical protein, at 91 to 1,083 GFF1 GFF2 - Transcription accessory protein (S1 RNA-binding domain), at 1,164 to 3,539 GFF2 Position (kb) 0 1 2Strain fitness (log2 ratio) -2 -1 0 1 2at 0.063 kb on + strandat 0.063 kb on + strandat 0.063 kb on + strandat 0.063 kb on + strandat 0.063 kb on + strandat 0.116 kb on + strandat 0.117 kb on - strandat 0.117 kb on - strandat 0.139 kb on + strandat 0.139 kb on + strandat 0.139 kb on + strandat 0.139 kb on + strandat 0.139 kb on + strandat 0.139 kb on + strandat 0.139 kb on + strandat 0.139 kb on + strandat 0.139 kb on + strandat 0.139 kb on + strandat 0.140 kb on - strandat 0.140 kb on - strandat 0.140 kb on - strandat 0.140 kb on - strandat 0.140 kb on - strandat 0.140 kb on - strandat 0.140 kb on - strandat 0.140 kb on - strandat 0.140 kb on - strandat 0.379 kb on + strand, within GFF1at 0.379 kb on + strand, within GFF1at 0.379 kb on + strand, within GFF1at 0.380 kb on - strand, within GFF1at 0.380 kb on - strand, within GFF1at 0.380 kb on - strand, within GFF1at 0.380 kb on - strand, within GFF1at 0.380 kb on - strand, within GFF1at 0.380 kb on - strand, within GFF1at 0.380 kb on - strand, within GFF1at 0.380 kb on - strand, within GFF1at 1.081 kb on + strandat 1.081 kb on + strandat 1.081 kb on + strandat 1.081 kb on + strandat 1.081 kb on + strandat 1.081 kb on + strandat 1.082 kb on - strandat 1.082 kb on - strandat 1.082 kb on - strandat 1.082 kb on - strandat 1.082 kb on - strandat 1.123 kb on + strandat 1.124 kb on - strandat 1.124 kb on - strandat 1.341 kb on + strandat 1.341 kb on + strandat 1.342 kb on - strandat 1.342 kb on - strandat 1.467 kb on + strand, within GFF2at 1.467 kb on + strand, within GFF2at 1.467 kb on + strand, within GFF2at 1.467 kb on + strand, within GFF2at 1.467 kb on + strand, within GFF2at 1.467 kb on + strand, within GFF2at 1.467 kb on + strand, within GFF2at 1.467 kb on + strand, within GFF2at 1.467 kb on + strand, within GFF2at 1.467 kb on + strand, within GFF2at 1.467 kb on + strand, within GFF2at 1.467 kb on + strand, within GFF2at 1.467 kb on + strand, within GFF2at 1.467 kb on + strand, within GFF2at 1.468 kb on - strand, within GFF2at 1.468 kb on - strand, within GFF2at 1.468 kb on - strand, within GFF2at 1.468 kb on - strand, within GFF2at 1.468 kb on - strand, within GFF2at 1.468 kb on - strand, within GFF2at 1.468 kb on - strand, within GFF2at 1.468 kb on - strand, within GFF2at 1.468 kb on - strand, within GFF2at 1.468 kb on - strand, within GFF2at 1.468 kb on - strand, within GFF2at 1.468 kb on - strand, within GFF2at 1.468 kb on - strand, within GFF2at 1.468 kb on - strand, within GFF2at 1.470 kb on - strand, within GFF2at 1.816 kb on - strand, within GFF2at 1.816 kb on - strand, within GFF2at 1.816 kb on - strand, within GFF2at 1.824 kb on + strand, within GFF2at 1.825 kb on - strand, within GFF2at 1.825 kb on - strand, within GFF2at 1.825 kb on - strand, within GFF2

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
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63 + -0.2
63 + -1.0
63 + +0.0
63 + +0.2
63 + -0.5
116 + +0.3
117 - -1.9
117 - +1.0
139 + +1.2
139 + -0.1
139 + +0.4
139 + +0.1
139 + -0.4
139 + -1.0
139 + -0.2
139 + -0.4
139 + -0.2
139 + +0.5
140 - -1.7
140 - -0.7
140 - +0.0
140 - +0.2
140 - +0.2
140 - +0.3
140 - -0.3
140 - -1.4
140 - -0.2
379 + GFF1 0.29 -0.0
379 + GFF1 0.29 +1.0
379 + GFF1 0.29 -0.8
380 - GFF1 0.29 +0.2
380 - GFF1 0.29 -0.1
380 - GFF1 0.29 -0.2
380 - GFF1 0.29 -1.0
380 - GFF1 0.29 -0.4
380 - GFF1 0.29 +0.4
380 - GFF1 0.29 -0.1
380 - GFF1 0.29 +1.4
1,081 + +0.3
1,081 + -1.2
1,081 + -0.8
1,081 + -0.5
1,081 + +0.7
1,081 + +0.0
1,082 - +1.4
1,082 - -1.7
1,082 - +0.1
1,082 - -0.5
1,082 - -0.1
1,123 + -1.4
1,124 - +1.8
1,124 - +0.5
1,341 + -0.0
1,341 + -0.8
1,342 - -0.4
1,342 - -2.5
1,467 + GFF2 0.13 -0.2
1,467 + GFF2 0.13 +0.8
1,467 + GFF2 0.13 -0.1
1,467 + GFF2 0.13 +1.1
1,467 + GFF2 0.13 -0.3
1,467 + GFF2 0.13 -0.9
1,467 + GFF2 0.13 -2.1
1,467 + GFF2 0.13 -0.5
1,467 + GFF2 0.13 -0.2
1,467 + GFF2 0.13 -1.8
1,467 + GFF2 0.13 -0.1
1,467 + GFF2 0.13 -0.2
1,467 + GFF2 0.13 -2.3
1,467 + GFF2 0.13 -0.1
1,468 - GFF2 0.13 +1.5
1,468 - GFF2 0.13 +0.5
1,468 - GFF2 0.13 -0.6
1,468 - GFF2 0.13 -0.4
1,468 - GFF2 0.13 -0.3
1,468 - GFF2 0.13 -0.5
1,468 - GFF2 0.13 -0.9
1,468 - GFF2 0.13 -0.5
1,468 - GFF2 0.13 -0.1
1,468 - GFF2 0.13 +0.2
1,468 - GFF2 0.13 -0.6
1,468 - GFF2 0.13 +0.5
1,468 - GFF2 0.13 -0.5
1,468 - GFF2 0.13 -0.1
1,470 - GFF2 0.13 +0.2
1,816 - GFF2 0.27 -0.7
1,816 - GFF2 0.27 -1.1
1,816 - GFF2 0.27 -1.7
1,824 + GFF2 0.28 +0.7
1,825 - GFF2 0.28 -1.8
1,825 - GFF2 0.28 -0.4
1,825 - GFF2 0.28 +1.1

Or see this region's nucleotide sequence